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Ucp1 (NM_009463) Mouse Tagged ORF Clone
CAT＃: MG204339
- TrueORF®
Ucp1 (tGFP-tagged) - Mouse uncoupling protein 1 (mitochondrial, proton carrier) (Ucp1)
ORF Plasmid: DDK
"NM_009463" in other vectors (4)
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CNY 4,000.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI385626; Slc25a7; Ucp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204339 representing NM_009463
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAACCCGACAACTTCCGAAGTGCAACCCACCATGGGGGTCAAGATCTTCTCAGCCGGAGTTTCAG CTTGCCTGGCAGATATCATCACCTTCCCGCTGGACACTGCCAAAGTCCGCCTTCAGATCCAAGGTGAAGG CCAGGCTTCCAGTACCATTAGGTATAAAGGTGTCCTAGGGACCATCACCACCCTGGCAAAAACAGAAGGA TTGCCGAAACTGTACAGCGGTCTGCCTGCGGGCATTCAGAGGCAAATCAGCTTTGCCTCACTCAGGATTG GCCTCTACGACTCAGTCCAAGAGTACTTCTCTTCAGGGAGAGAAACACCTGCCTCTCTCGGAAACAAGAT CTCAGCCGGCTTAATGACTGGAGGTGTGGCAGTGTTCATTGGGCAGCCTACAGAGGTCGTGAAGGTCAGA ATGCAAGCCCAGAGCCATCTGCATGGGATCAAACCCCGCTACACGGGGACCTACAATGCTTACAGAGTTA TAGCCACCACAGAAAGCTTGTCAACACTTTGGAAAGGGACGACCCCTAATCTAATGAGAAATGTCATCAT CAATTGTACAGAGCTGGTAACATATGACCTCATGAAGGGGGCCCTTGTAAACAACAAAATACTGGCAGAT GACGTCCCCTGCCATTTACTGTCAGCTCTTGTTGCCGGGTTTTGCACCACACTCCTGGCCTCTCCAGTGG ATGTGGTAAAAACAAGATTCATCAACTCTCTGCCAGGACAGTACCCAAGCGTACCAAGCTGTGCGATGTC CATGTACACCAAGGAAGGACCGACGGCCTTTTTCAAAGGGTTTGTGGCTTCTTTTCTGCGACTCGGGTCC TGGAACGTCATCATGTTTGTGTGCTTTGAACAGCTGAAAAAAGAGCTGATGAAGTCCAGACAGACAGTGG ATTGTACCACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204339 representing NM_009463
Red=Cloning site Green=Tags(s) MVNPTTSEVQPTMGVKIFSAGVSACLADIITFPLDTAKVRLQIQGEGQASSTIRYKGVLGTITTLAKTEG LPKLYSGLPAGIQRQISFASLRIGLYDSVQEYFSSGRETPASLGNKISAGLMTGGVAVFIGQPTEVVKVR MQAQSHLHGIKPRYTGTYNAYRVIATTESLSTLWKGTTPNLMRNVIINCTELVTYDLMKGALVNNKILAD DVPCHLLSALVAGFCTTLLASPVDVVKTRFINSLPGQYPSVPSCAMSMYTKEGPTAFFKGFVASFLRLGS WNVIMFVCFEQLKKELMKSRQTVDCTT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009463 |
| ORF Size | 921 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009463.3 |
| RefSeq Size | 1249 bp |
| RefSeq ORF | 924 bp |
| Locus ID | 22227 |
| UniProt ID | P12242 |
| Gene Summary | Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates to non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (PubMed:9139827, PubMed:19187776, PubMed:23063128, PubMed:27027295). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP (PubMed:23063128). Regulates the production of reactive oxygen species/ROS by mitochondria (PubMed:20416274, PubMed:20466728).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200793 | Ucp1 (untagged) - Mouse uncoupling protein 1 (mitochondrial, proton carrier) (Ucp1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MR204339 | Ucp1 (Myc-DDK-tagged) - Mouse uncoupling protein 1 (mitochondrial, proton carrier) (Ucp1), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |
|
| MR204339L3 | Lenti ORF clone of Ucp1 (Myc-DDK-tagged) - Mouse uncoupling protein 1 (mitochondrial, proton carrier) (Ucp1), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR204339L4 | Lenti ORF clone of Ucp1 (mGFP-tagged) - Mouse uncoupling protein 1 (mitochondrial, proton carrier) (Ucp1), nuclear gene encoding mitochondrial protein |
CNY 4,800.00 |
