Gpr35 (NM_022320) Mouse Tagged ORF Clone
CAT#: MG204323
- TrueORF®
Gpr35 (tGFP-tagged) - Mouse G protein-coupled receptor 35 (Gpr35)
"NM_022320" in other vectors (5)
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CNY 2,850.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | KPG_007 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204323 representing NM_022320
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAGTACAACCTGTAACAGCACCCTCACGTGGCCTGCTTCCGTCAACAACTTCTTCATCATCTACT CAGCCTTGCTGCTGGTGCTGGGCCTGCTGCTCAACAGCGTGGCACTCTGGGTATTCTGCTATCGCATGCA CCAGTGGACAGAGACCCGCATCTATATGACCAACCTGGCTGTGGCCGACCTCTGCCTGCTCTGCTCCTTG CCATTTGTGCTGTACTCCCTGAAATATAGTTCTTCAGACACACCCGTCTGCCAGCTCTCACAGGGCATCT ACCTGGCCAACAGATACATGAGCATAAGCCTGGTCACTGCCATTGCTGTGGACCGCTATGTGGCAGTGCG GCATCCCCTGCGTGCGCGTGAGCTGCGGTCCCCGAGACAGGCTGCAGCAGTGTGTGTGGCCCTTTGGGTG ATAGTGGTCACCTCCCTGGTAGTGCGCTGGCGCCTGGGGATGCAGGAGGGTGGCTTCTGCTTCAGCAGCC AAACCCGGCGCAATTTCAGCACCACTGCCTTCTCACTGCTGGGATTCTACCTGCCGCTGGCCATCGTGGT CTTCTGCTCTTTGCAGGTAGTGACTGTGCTATCGAGAAGGCCAGCCGCTGATGTGGGGCAGGCAGAGGCC ACCCAAAAGGCCACCCACATGGTCTGGGCCAACTTGGCTGTGTTTGTCATCTGCTTCCTGCCCTTGCATG TGGTCCTGACCGTGCAGGTCTCCCTGAACCTCAATACCTGTGCTGCCCGAGACACCTTCAGCCGTGCCCT GTCCATCACAGGTAAACTCTCAGACACCAACTGCTGCCTGGATGCCATCTGTTACTACTACATGGCCAGA GAGTTCCAGGAAGCGTCCAAGCCAGCCACGTCTTCCAACACACCCCACAAGAGCCAAGATTCCCAGATCC TGAGCCTCACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204323 representing NM_022320
Red=Cloning site Green=Tags(s) MNSTTCNSTLTWPASVNNFFIIYSALLLVLGLLLNSVALWVFCYRMHQWTETRIYMTNLAVADLCLLCSL PFVLYSLKYSSSDTPVCQLSQGIYLANRYMSISLVTAIAVDRYVAVRHPLRARELRSPRQAAAVCVALWV IVVTSLVVRWRLGMQEGGFCFSSQTRRNFSTTAFSLLGFYLPLAIVVFCSLQVVTVLSRRPAADVGQAEA TQKATHMVWANLAVFVICFLPLHVVLTVQVSLNLNTCAARDTFSRALSITGKLSDTNCCLDAICYYYMAR EFQEASKPATSSNTPHKSQDSQILSLT TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_022320 |
ORF Size | 4230 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_022320.4, NP_071715.3 |
RefSeq Size | 3158 bp |
RefSeq ORF | 924 bp |
Locus ID | 64095 |
UniProt ID | Q9ES90 |
Gene Summary | Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203180 | Gpr35 (untagged) - Mouse G protein-coupled receptor 35 (Gpr35), transcript variant 1, (10ug) |
CNY 2,400.00 |
|
MG219999 | Gpr35 (tGFP-tagged) - Mouse G protein-coupled receptor 35 (Gpr35) transcript variant 1, (10ug) |
CNY 4,000.00 |
|
MR219999 | Gpr35 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 35 (Gpr35), transcript variant 1 |
CNY 2,400.00 |
|
MR219999L3 | Lenti ORF clone of Gpr35 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 35 (Gpr35), transcript variant 1 |
CNY 4,750.00 |
|
MR219999L4 | Lenti ORF clone of Gpr35 (mGFP-tagged) - Mouse G protein-coupled receptor 35 (Gpr35), transcript variant 1 |
CNY 4,750.00 |