Fbxo22 (NM_028049) Mouse Tagged ORF Clone
CAT#: MG204160
- TrueORF®
Fbxo22 (tGFP-tagged) - Mouse F-box protein 22 (Fbxo22)
ORF Plasmid: DDK
"NM_028049" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 0610033L19Rik; 1600016C16Rik |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204160 representing NM_028049
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGATTCTGAAACTTTCATCAGCCTGGAAGAGTGTCGTGGCCATAAAAGAGCGAGGAAGAGAACTA CTATGGAGACAGCATGTGCCCTGGAGAAGCTTTTCCCCAAGCAGTGCCAAGTCCTTGGGATTGTGACCCC GGGAATTGTGGTGACTCCAATGGGATCAGGTAGCAATCGACCTCAGGAAATAGAAATTGGAGAATCTGGT TTTGCTTTATTATTCCCTCAAATTGAAGGAATAAAAATTCAACCCTTTCATTTTATTAAGGACTCCAAGA ATTTAACACTCGAAAGACACCAGCTTACTGAAGTAGGTCTTCTGGACAACCCTGAACTTCGTGTGGTCCT TGTCTTTGGCTATAACTGCTGTAAGGTGGGAGCCAGTAATTACCTGCATCGGGTAGTCAGCACTTTCAGC GATATGAATATCATCTTGGCTGGAGGCCAGGTGGACAATTTGTCTTCACTTACTTGTGAGAAGAACCCTC TGGATATTGATGCCACAGGTGTGGTTGGACTGTCATTTAGTGGGCACCGAATCCAGAGTGCCACAGTTCT CCTCACTGAGGATGTAAATGATGCCAAGACCGTCGAGGCTGCTATGCAGCGCCTCAAAGCAGCCAACATC CCTGAACAGAACACCATTGGCTTCATGTTTGCATGCGTTGGCCGAGGCTTTCAGTACTACAGAGCCAAGG GGAATGTTGAAGCTGATGCATTTAGAAAGTTCTTTCCCAGTGTCCCCTTATTTGGCTTCTTTGGAAATGG GGAGATTGGCTGCGACCGGATAGTCACTGGGAACTTTATACTGAGGAGATGTAATGAGGTAAAGGAAGAG GACCTGTTCCATAGCTACACAACCATCATGGCTCTCGTTCACCTGGGAACCTCTAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204160 representing NM_028049
Red=Cloning site Green=Tags(s) MADSETFISLEECRGHKRARKRTTMETACALEKLFPKQCQVLGIVTPGIVVTPMGSGSNRPQEIEIGESG FALLFPQIEGIKIQPFHFIKDSKNLTLERHQLTEVGLLDNPELRVVLVFGYNCCKVGASNYLHRVVSTFS DMNIILAGGQVDNLSSLTCEKNPLDIDATGVVGLSFSGHRIQSATVLLTEDVNDAKTVEAAMQRLKAANI PEQNTIGFMFACVGRGFQYYRAKGNVEADAFRKFFPSVPLFGFFGNGEIGCDRIVTGNFILRRCNEVKEE DLFHSYTTIMALVHLGTSK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028049 |
ORF Size | 897 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028049.1, NP_082325.1 |
RefSeq Size | 2057 bp |
RefSeq ORF | 1209 bp |
Locus ID | 71999 |
UniProt ID | Q78JE5 |
Gene Summary | Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Promotes the proteasome-dependent degradation of key sarcomeric proteins, such as alpha-actinin (ACTN2) and filamin-C (FLNC), essential for maintenance of normal contractile function.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202928 | Fbxo22 (untagged) - Mouse F-box protein 22 (Fbxo22), (10ug) |
CNY 2,400.00 |
|
MR204160 | Fbxo22 (Myc-DDK-tagged) - Mouse F-box protein 22 (Fbxo22) |
CNY 2,400.00 |
|
MR204160L3 | Lenti ORF clone of Fbxo22 (Myc-DDK-tagged) - Mouse F-box protein 22 (Fbxo22) |
CNY 4,750.00 |
|
MR204160L4 | Lenti ORF clone of Fbxo22 (mGFP-tagged) - Mouse F-box protein 22 (Fbxo22) |
CNY 4,750.00 |