Atxn3 (BC087880) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	ATX3, MJD1, ataxin-3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204111 representing BC087880 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCCATCTTCCACGAGAAACAAGAAGGCTCACTTTGTGCTCAGCATTGCCTGAATAACCTATTGC AAGGAGAGTATTTTAGCCCTGTGGAGCTATCCTCAATTGCACACCAGCTGGATGAAGAGGAGAGGCTGAG AATGGCAGAAGGGGGAGTCACTAGTGAAGACTACCGCACATTTTTACAGCAGCCTTCTGGAAATATGGAT GACAGCGGCTTTTTCTCTATTCAAGTTATAAGCAATGCTTTGAAAGTTTGGGGTTTAGAACTAATCCTGT TCAACAGTCCAGAGTACCAGAGGCTCAGAATTGATCCTATAAACGAAAGATCCTTTATATGCAATTATAA AGAACACTGGTTTACAGTTAGAAAATTAGGCAAGCAGTGGTTTAACTTGAATTCTCTGTTGACGGGTCCA GAATTAATATCAGATACATACCTCGCACTATTCTTGGCTCAATTACAGCAAGAAGGTTATTCTATATTTG TTGTTAAGGGTGATCTGCCAGATTGTGAAGCTGACCAACTTTTGCAGATGATCAAGGTCCAACAGATGCA TCGACCAAAACTTATTGGAGAGGAACTTGCACATCTGAAAGAGCAGAGTGCCCTCAAAGCAGACCTGGAG CGCGTCTTAGAAGCAGCTGATGGGTCGGGCATATTTGATGAAGATGAGGATGATTTACAGAGGGCTCTAG CCATAAGTCGCCAGGAAATCGACATGGAGGATGAAGAAGCTGATCTCCGCAGGGCCATTCAGCTCAGTAT GCAAGTTCCAGAAGTATGTGTGAAAATAGTCCACAGACATCAAGTCCAGATCTCTCTTCAGAAGAGCTGC GGAGGAGACGAGAAGCCTACTTTGAAAAGTAAAGTAGTTGGTACAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204111 representing BC087880 Red=Cloning site Green=Tags(s) MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERLRMAEGGVTSEDYRTFLQQPSGNMD DSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGP ELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIKVQQMHRPKLIGEELAHLKEQSALKADLE RVLEAADGSGIFDEDEDDLQRALAISRQEIDMEDEEADLRRAIQLSMQVPEVCVKIVHRHQVQISLQKSC GGDEKPTLKSKVVGTD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC087880
ORF Size	890 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC087880, AAH87880
RefSeq Size	1083 bp
RefSeq ORF	890 bp
Locus ID	110616
Gene Summary	Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates (By similarity). Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins (By similarity). Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension (PubMed:21855799). Interacts with key regulators of transcription and represses transcription: acts as a histone-binding protein that regulates transcription (By similarity). Regulates autophagy via the deubiquitination of 'Lys-402' of BECN1 leading to the stabilization of BECN1 (PubMed:28445460).[UniProtKB/Swiss-Prot Function]

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