Aqp3 (NM_016689) Mouse Tagged ORF Clone
CAT#: MG203989
- TrueORF®
Aqp3 (tGFP-tagged) - Mouse aquaporin 3 (Aqp3)
ORF Plasmid: DDK
"NM_016689" in other vectors (4)
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CNY 4,000.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AQP-2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203989 representing NM_016689
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCGACAGAAGGAGTTGATGAATCGTTGTGGGGAGATGCTTCACATCCGCTACCGGCTGCTTCGCC AGGCGCTGGCGGAGTGCCTGGGGACCCTCATCCTTGTGATGTTTGGCTGTGGCTCCGTGGCTCAGGTGGT GCTCAGCCGTGGCACCCATGGTGGCTTCCTCACCATCAACTTGGCTTTTGGCTTCGCTGTCACCCTTGGC ATCTTGGTGGCTGGCCAGGTGTCTGGTGCCCACTTGAACCCCGCTGTGACCTTCGCAATGTGCTTCCTGG CACGAGAGCCCTGGATCAAGCTGCCCATCTATGCACTGGCACAGACACTGGGGGCCTTCTTGGGCGCTGG GATTGTTTTTGGGCTGTACTACGATGCAATCTGGGCCTTTGCCAACAATGAGCTTTTCGTCTCTGGCCCC AACGGCACAGCTGGAATCTTTGCCACCTATCCCTCTGGACACTTGGACATGGTCAATGGCTTCTTTGATC AGTTCATAGGCACAGCCGCCCTTATTGTGTGTGTACTGGCCATCGTTGACCCTTATAACAACCCTGTGCC CCGTGGCCTGGAGGCTTTCACTGTGGGCCTGGTGGTCCTGGTCATTGGAACCTCCATGGGCTTCAATTCT GGCTATGCCGTCAACCCTGCCCGTGACTTTGGACCTCGCCTCTTCACCGCCCTGGCTGGCTGGGGCTCAG AAGTCTTCACGACTGGCCGGCACTGGTGGTGGGTACCCATTGTCTCCCCACTCCTGGGTTCCATCGCTGG TGTCTTCGTGTACCAGCTCATGATTGGTTGCCACCTGGAGCAGCCCCCACCCTCCACCGAGGAAGAGAAT GTGAAGCTGGCCCACATGAAACACAAGGAGCAGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203989 representing NM_016689
Red=Cloning site Green=Tags(s) MGRQKELMNRCGEMLHIRYRLLRQALAECLGTLILVMFGCGSVAQVVLSRGTHGGFLTINLAFGFAVTLG ILVAGQVSGAHLNPAVTFAMCFLAREPWIKLPIYALAQTLGAFLGAGIVFGLYYDAIWAFANNELFVSGP NGTAGIFATYPSGHLDMVNGFFDQFIGTAALIVCVLAIVDPYNNPVPRGLEAFTVGLVVLVIGTSMGFNS GYAVNPARDFGPRLFTALAGWGSEVFTTGRHWWWVPIVSPLLGSIAGVFVYQLMIGCHLEQPPPSTEEEN VKLAHMKHKEQI TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016689 |
ORF Size | 876 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_016689.2, NP_057898.2 |
RefSeq Size | 1763 bp |
RefSeq ORF | 879 bp |
Locus ID | 11828 |
UniProt ID | Q8R2N1 |
Gene Summary | Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204210 | Aqp3 (untagged) - Mouse aquaporin 3 (Aqp3), (10ug) |
CNY 2,400.00 |
|
MR203989 | Aqp3 (Myc-DDK-tagged) - Mouse aquaporin 3 (Aqp3) |
CNY 2,400.00 |
|
MR203989L3 | Lenti ORF clone of Aqp3 (Myc-DDK-tagged) - Mouse aquaporin 3 (Aqp3) |
CNY 4,750.00 |
|
MR203989L4 | Lenti ORF clone of Aqp3 (mGFP-tagged) - Mouse aquaporin 3 (Aqp3) |
CNY 4,750.00 |