Stx1a (NM_016801) Mouse Tagged ORF Clone
CAT#: MG203927
- TrueORF®
Stx1a (tGFP-tagged) - Mouse syntaxin 1A (brain) (Stx1a)
ORF Plasmid: DDK
"NM_016801" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | HPC-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203927 representing NM_016801
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGACCGAACCCAGGAGCTCCGCACGGCCAAGGACAGCGATGACGACGACGATGTCACTGTCACTG TGGACCGAGACCGCTTCATGGATGAATTCTTTGAACAGGTGGAAGAGATCCGGGGCTTTATTGACAAGAT TGCCGAAAACGTGGAGGAGGTGAAGCGGAAACACAGCGCCATCCTGGCCTCCCCGAACCCCGATGAGAAG ACAAAGGAGGAACTGGAGGAGCTCATGTCGGACATTAAGAAGACAGCGAACAAAGTTCGCTCCAAGCTAA AGAGCATTGAGCAGAGCATCGAGCAGGAGGAAGGTCTGAACCGCTCATCAGCCGACCTGAGGATCCGGAA GACGCAGCACTCCACGCTGTCCCGAAAGTTTGTGGAGGTCATGTCCGAGTACAACGCCACTCAGTCAGAC TACCGAGAACGCTGCAAAGGGCGCATCCAGAGGCAGCTGGAGATCACCGGCCGGACCACGACCAGTGAGG AATTGGAAGACATGCTGGAGAGTGGGAACCCTGCCATCTTTGCCTCTGGGATCATCATGGACTCCAGCAT CTCGAAGCAGGCCCTCAGTGAGATCGAGACCAGACACAGTGAGATCATCAAGCTGGAGACCAGCATCCGG GAGCTGCACGACATGTTCATGGACATGGCCATGCTGGTGGAGAGCCAGGGGGAGATGATTGACAGGATCG AGTACAATGTGGAGCACGCCGTGGACTACGTGGAGAGGGCCGTGTCAGACACCAAGAAGGCCGTCAAGTA CCAGAGCAAGGCGCGCAGGAAGAAGATCATGATCATCATTTGCTGTGTGATTCTGGGCATCATCATCGCC TCCACCATCGGGGGCATCTTTGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203927 representing NM_016801
Red=Cloning site Green=Tags(s) MKDRTQELRTAKDSDDDDDVTVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEK TKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNATQSD YRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASGIIMDSSISKQALSEIETRHSEIIKLETSIR ELHDMFMDMAMLVESQGEMIDRIEYNVEHAVDYVERAVSDTKKAVKYQSKARRKKIMIIICCVILGIIIA STIGGIFG TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016801 |
ORF Size | 864 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016801.4, NP_058081.2 |
RefSeq Size | 2071 bp |
RefSeq ORF | 867 bp |
Locus ID | 20907 |
UniProt ID | O35526 |
Gene Summary | Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis (PubMed:17502420, PubMed:28596237, PubMed:28031464). Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm (PubMed:12101244). Plays also an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1) (PubMed:17502420, PubMed:28596237, PubMed:28031464).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209479 | Stx1a (untagged) - Mouse syntaxin 1A (brain) (Stx1a), (10ug) |
CNY 3,990.00 |
|
MR203927 | Stx1a (Myc-DDK-tagged) - Mouse syntaxin 1A (brain) (Stx1a) |
CNY 1,416.00 |
|
MR203927L3 | Lenti ORF clone of Stx1a (Myc-DDK-tagged) - Mouse syntaxin 1A (brain) (Stx1a) |
CNY 4,750.00 |
|
MR203927L4 | Lenti ORF clone of Stx1a (mGFP-tagged) - Mouse syntaxin 1A (brain) (Stx1a) |
CNY 4,750.00 |