Haghl (BC030466) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1500017E18Rik; 2810014I23Rik; C330022E15Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203845 representing BC030466 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTCAAAGTCATCCCGGTACTTGAAGACAACTACATGTACCTGATCATTGAGGAACACACGCGGG AGGCCGTAGCTATAGACGTGGCTGTGGCCGAGAGGTTGCTGGAGATTGCGGGTCGCGAGGGAGTGTCTCT GACCATGGTGCTGTCCACCCATCATCACTGGGACCACACACGTGGAAATGCGGAGCTGGCACACATTCTG CCAGGATTGGCTGTCTTGGGAGCAGATGAGCGCATTTGCGCTCTAACCCGAAGGCTGGAACATGGAGAAG GGCTTCAGTTTGGGGCCATCCACGTGCGATGCCTCTTGACGCCAGGCCACACCTCTGGCCACATGAGCTA CTTCCTGTGGGAGGATGACTGCCCGGACTCACCAGCCCTCTTCTCTGGGGATGCTCTGTCAGTGGCAGGC TGTGGCTGGCACCTGGAGGACACTGCCCAGCAGATGTACCAGAGCTTGGCCAAGACTCTGGGTACCCTTC CACCCGAGACGAAGGTGTTCTGTGGTCATGAGCATACGCTGAGCAATCTTGAGTTTGCACAGAAAGTGGA GCCCTGCAACGAACACGTGCAAGCTAAGCTGTCCTGGGCCCAGGAGAGGGATGACGAGGATATTCCTACT GTGCCTTCGACGCTGGGCGAGGAGCTCATGTACAACCCTTTCCTGAGGGTGACAGAGGATGCAGTCCGTG CGTTCACAGGCCAGGTGGCCCCAGCCCAGGTCCTGGAGGCCCTCTGCAGGGAGCGAGCGCGCTTCCAGCC TGCTGTGGAGCCACCACAGCCGCAGGTTCGGGCACTCCTGGCCTTACAGTGGGGGCTTCTGAGCACACAC CAGAAAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203845 representing BC030466 Red=Cloning site Green=Tags(s) MKVKVIPVLEDNYMYLIIEEHTREAVAIDVAVAERLLEIAGREGVSLTMVLSTHHHWDHTRGNAELAHIL PGLAVLGADERICALTRRLEHGEGLQFGAIHVRCLLTPGHTSGHMSYFLWEDDCPDSPALFSGDALSVAG CGWHLEDTAQQMYQSLAKTLGTLPPETKVFCGHEHTLSNLEFAQKVEPCNEHVQAKLSWAQERDDEDIPT VPSTLGEELMYNPFLRVTEDAVRAFTGQVAPAQVLEALCRERARFQPAVEPPQPQVRALLALQWGLLSTH QKK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC030466
ORF Size	851 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC030466, AAH30466
RefSeq Size	1308 bp
RefSeq ORF	851 bp
Locus ID	68977
Gene Summary	Hydrolase acting on ester bonds.[UniProtKB/Swiss-Prot Function]

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