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Tmem25 (BC060057) Mouse Tagged ORF Clone
CAT＃: MG203767
- TrueORF®
Tmem25 (tGFP-tagged) - Mouse transmembrane protein 25 (cDNA clone MGC:64798 IMAGE:5696102)
ORF Plasmid: DDK
"BC060057" in other vectors (4)
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CNY 5,200.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 0610039J01Rik; AI429491 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203767 representing BC060057
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATTGCCTCTAAGCCAAGCTACCCTCCGGCACACACTGCTGCTCCTTCCTGCCCTCTTAAGTTCAG GTCAAGGGGAGCTGGCGCCGCAAATTGATGGTCAGACCTGGGCTGAGAGAGCGCTTAGGGAGAATGAGCA CCATGCCTTCACCTGTCGGGTTGCAGGAGGTTCTGCCACTCCCCGATTAGCCTGGTACCTGGATGGACAG CTACAGGAGGCCACCACCTCCCGACTGCTGAGTGTGGGTGGGGACGCCTTCTCTGGAGGAACCAGCACTT TCACTGTTACTGCCCAGCGCTCCCAACATGAGCTTAACTGCTCCCTGCAGGACCCAGGGAGTGGCCGGCC AGCCAATGCCTCTGTCATCCTCAATGTGCAATTCAAACCAGAGATTGCCCAGGTTGGAGCCAAATACCAG GAAGCTCAGGGCCCAGGCCTTCTGGTTGTCCTGTTTGCCCTGGTGCGGGCCAACCCACCTGCCAATGTCA CCTGGATTGACCAGGATGGGCCAGTGACTGTCAATGCCTCTGACTTCCTGGTGCTGGATGCTCAGAACTA TCCCTGGCTCACCAACCACACTGTGCAGCTGCAGCTCCGGAGCTTGGCACACAACCTCTCGGTGGTGGCC ACCAATGACGTGGGTGTCACTAGCGCTTCCCTTCCAGCTCCAGGGCTCCTGGCCACACGGATAGAAGTGC CACTGCTGGGCATCGTTGTGGCTGGAGGACTTGCCTTGGGCACTCTAGTGGGGTTCAGTACCTTGGTGGC CTGCCTGGTCTGCAGGAAAGAGAAGAAAACAAAAGGCCCCTCCCGGCGTCCTTCTCTGATATCTAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203767 representing BC060057
Red=Cloning site Green=Tags(s) MELPLSQATLRHTLLLLPALLSSGQGELAPQIDGQTWAERALRENEHHAFTCRVAGGSATPRLAWYLDGQ LQEATTSRLLSVGGDAFSGGTSTFTVTAQRSQHELNCSLQDPGSGRPANASVILNVQFKPEIAQVGAKYQ EAQGPGLLVVLFALVRANPPANVTWIDQDGPVTVNASDFLVLDAQNYPWLTNHTVQLQLRSLAHNLSVVA TNDVGVTSASLPAPGLLATRIEVPLLGIVVAGGLALGTLVGFSTLVACLVCRKEKKTKGPSRRPSLISR TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060057 |
| ORF Size | 837 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060057, AAH60057 |
| RefSeq Size | 2714 bp |
| RefSeq ORF | 839 bp |
| Locus ID | 71687 |
| Gene Summary | In neurons, modulates the degradation of NMDA receptor GRIN2B subunit. Plays a role in the regulation of neuronal excitability.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217876 | Tmem25 (untagged) - Mouse transmembrane protein 25 (cDNA clone MGC:64798 IMAGE:5696102), (10ug) |
CNY 6,940.00 |
|
| MR203767 | Tmem25 (Myc-DDK-tagged) - Mouse transmembrane protein 25 (cDNA clone MGC:64798 IMAGE:5696102) |
CNY 3,600.00 |
|
| MR203767L3 | Lenti ORF clone of Tmem25 (Myc-DDK-tagged) - Mouse transmembrane protein 25 (cDNA clone MGC:64798 IMAGE:5696102) |
CNY 5,890.00 |
|
| MR203767L4 | Lenti ORF clone of Tmem25 (mGFP-tagged) - Mouse transmembrane protein 25 (cDNA clone MGC:64798 IMAGE:5696102) |
CNY 5,890.00 |
