Cops7a (NM_012003) Mouse Tagged ORF Clone
CAT#: MG203690
- TrueORF®
Cops7a (tGFP-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 7a (Arabidopsis thaliana) (Cops7a)
ORF Plasmid: DDK
"NM_012003" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | D6Ertd35e; SGN7a |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203690 representing NM_012003
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCGGAGGTGAAGGTGACAGGGCAGAACCAAGAGCAGTTTCTGCTCCTTGCCAAGTCGGCTAAGG GGGCGGCACTGGCCACACTCATCCACCAGGTGCTGGAGGCCCCTGGTGTCTACGTGTTTGGGGAACTGCT GGATATGCCTAATGTTAGAGAGCTGGCAGAAAGCGACTTTGCCTCCACCTTCCGGCTGCTCACAGTGTTT GCCTATGGGACCTATGCGGACTACTTAGCTGAAGCCAGGAATCTCCCCCCACTGACTGACGCACAGAAGA ATAAGCTTCGACATCTGTCAGTTGTCACTCTGGCTGCCAAAGTCAAGTGTATCCCATATGCAGTGTTGCT GGAGGCCCTTGCCCTTCGAAACGTGCGCCAGCTGGAAGACCTTGTGATCGAGGCTGTGTATGCTGATGTC CTTCGTGGCTCTCTGGACCAGCGCAATCAGCGGCTAGAGGTTGATTACAGCATCGGGCGGGACATCCAGC GCCAGGACCTCAGTGCCATCGCCCAGACCCTGCAAGAGTGGTGCGTGGGCTGTGAGGTTGTGTTGTCGGG CATCGAAGAGCAGGTCAGCCGTGCCAACCAGCACAAGGAGCAGCAGCTGGGCCTGAAGCAGCAGATCGAA AGTGAGGTTGCCAACCTTAAGAAAACCATTAAAGTTACGACAGCAGCTGCTGCTGCAGCCACCTCCCAGG ATCCTGAGCAACACCTGACAGAGCTGAGAGAACCAGCTTCTGGCACCAACCAGCGCCAGCCCAGCAAGAA AGCCTCCAAGGGCAAGGGACTCCGAGGGAGCGCCAAGATTTGGTCCAAGTCGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203690 representing NM_012003
Red=Cloning site Green=Tags(s) MSAEVKVTGQNQEQFLLLAKSAKGAALATLIHQVLEAPGVYVFGELLDMPNVRELAESDFASTFRLLTVF AYGTYADYLAEARNLPPLTDAQKNKLRHLSVVTLAAKVKCIPYAVLLEALALRNVRQLEDLVIEAVYADV LRGSLDQRNQRLEVDYSIGRDIQRQDLSAIAQTLQEWCVGCEVVLSGIEEQVSRANQHKEQQLGLKQQIE SEVANLKKTIKVTTAAAAAATSQDPEQHLTELREPASGTNQRQPSKKASKGKGLRGSAKIWSKSN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012003 |
ORF Size | 825 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012003.4 |
RefSeq Size | 1779 bp |
RefSeq ORF | 828 bp |
Locus ID | 26894 |
UniProt ID | Q9CZ04 |
Gene Summary | Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200375 | Cops7a (untagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 7a (Arabidopsis thaliana) (Cops7a), transcript variant 1, (10ug) |
CNY 2,000.00 |
|
MR203690 | Cops7a (Myc-DDK-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 7a (Arabidopsis thaliana) (Cops7a), transcript variant 1 |
CNY 1,416.00 |
|
MR203690L3 | Lenti ORF clone of Cops7a (Myc-DDK-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 7a (Arabidopsis thaliana) (Cops7a), transcript variant 1 |
CNY 4,750.00 |
|
MR203690L4 | Lenti ORF clone of Cops7a (mGFP-tagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 7a (Arabidopsis thaliana) (Cops7a), transcript variant 1 |
CNY 4,750.00 |