Prkab2 (BC060228) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5730553K21Rik; AW049591; BB124140
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203604 representing BC060228 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAACACTACCAGCGAGCGGGTGTCCGGGGAGCGCCACGGAGCCAAAGCTGCTCGAGCTGAGGGCG GCGGCCATGGCCCGGGAAAGGAGCACAAGATCATGGTGGGGAGCACGGACGACCCCAGCGTCTTCAGCCT GCCCGACTCCAAGCTCCCCGGGGACAAAGAGTTTGTACCCTGGCAGCAGGATTTGGATGATTCTGTGAAG CCCGCCCAGCAGGCCCGGCCCACCGTTATCCGCTGGTCTGAAGGAGGCAAGGAGGTCTTCATCTCTGGGT CCTTCAACAATTGGAGCACCAAGATCCCTCTGATAAAGAGTCATAATGACTTCGTTGCCATCCTGGATCT TCCAGAGGGAGAGCATCAGTACAAGTTCTTTGTGGACGGACAGTGGGTTCATGATCCGTCAGAGCCTGTG GTTACCAGTCAGCTTGGAACAATTAATAACTTGATCCACGTCAAGAAATCTGATTTTGAAGTATTTGACG CTTTAAAGTTAGACTCTATGGAAAGCTCGGAGACGTCATGTCGAGACCTGTCCAGCTCACCCCCTGGGCC TTACGGTCAAGAAATGTATGTGTTTCGATCTGAGGAGAGATTCAAATCCCCACCCATCCTGCCCCCTCAC CTACTCCAAGTTATTCTTAACAAGGACACGAATATTTCATGTGACCCAGCCTTACTTCCTGAGCCCAATC ATGTTATGCTGAACCATCTCTATGCACTGTCCATTAAGGACAGTGTGATGGTCCTTAGCGCAACCCATCG CTACAAGAAGAAGTATGTCACCACGCTGCTGTATAAGCCCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203604 representing BC060228 Red=Cloning site Green=Tags(s) MGNTTSERVSGERHGAKAARAEGGGHGPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVPWQQDLDDSVK PAQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPV VTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYVFRSEERFKSPPILPPH LLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC060228
ORF Size	815 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC060228, AAH60228
RefSeq Size	4278 bp
RefSeq ORF	815 bp
Locus ID	108097
Gene Summary	Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) (By similarity).[UniProtKB/Swiss-Prot Function]

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