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Psen1 (BC014744) Mouse Tagged ORF Clone
CAT＃: MG203433
- TrueORF®
Psen1 (tGFP-tagged) - Mouse presenilin 1 (cDNA clone MGC:25288 IMAGE:4511140)
ORF Plasmid: DDK
"BC014744" in other vectors (3)
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CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PS1, S182, PS-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203433 representing BC014744
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGAGATACCTGCACCTTTGTCCTACTTCCAGAATGCCCAGATGTCTGAGGACAGCCACTCCAGCA GCGCCATCCGGAGCCAGAATGACAGCCAAGAACGGCAGCAGCAGCATGACAGGCAGAGACTTGACAACCC TGAGCCAATATCTAATGGGCGGCCCCAGAGTAACTCAAGACAGGTGGTGGAACAAGATGAGGAGGAAGAC GAAGAGCTGACATTGAAATATGGAGCCAAGCATGTCATCATGCTCTTTGTCCCCGTGACCCTCTGCATGG TCGTCGTCGTGGCCACCATCAAATCAGTCAGCTTCTATACCCGGAAGGACGGTCAGCTAATCTACACCCC ATTCACAGAAGACACTGAGACTGTAGGCCAAAGAGCCCTGCACTCGATCCTGAATGCGGCCATCATGATC AGTGTCATTGTCATTATGACCATCCTCCTGGTGGTCCTGTATAAATACAGGTGCTACAAGGTCATCCACG CCTGGCTTATTATTTCATCTCTGTTGTTGCTGTTCTTTTTTTCGTTCATTTACTTAGGGGAAGTATTTAA GACCTACAATGTCGCCGTGGACTACGTTACAGTAGCACTCCTAATCTGGAATTTTGGTGTGGTCGGGATG ATTGCCATCCACTGGAAAGGCCCCCTTCGACTGCAGCAGGCGTATCTCATTATGATCAGTGCCCTCATGG CCCTGGTATTTATCAAGTACCTCCCCGAATGGACCGCATGGCTCATCTTGGCTGTGATTTCAGTATATGG TAAAGCTCAAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203433 representing BC014744
Red=Cloning site Green=Tags(s) MTEIPAPLSYFQNAQMSEDSHSSSAIRSQNDSQERQQQHDRQRLDNPEPISNGRPQSNSRQVVEQDEEED EELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTEDTETVGQRALHSILNAAIMI SVIVIMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFIYLGEVFKTYNVAVDYVTVALLIWNFGVVGM IAIHWKGPLRLQQAYLIMISALMALVFIKYLPEWTAWLILAVISVYGKAQD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC014744 |
| ORF Size | 785 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC014744, AAH14744 |
| RefSeq Size | 1982 bp |
| RefSeq ORF | 785 bp |
| Locus ID | 19164 |
| Gene Summary | Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the presence of the other members of the gamma-secretase complex for protease activity (By similarity). Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (PubMed:10421573, PubMed:11517342). Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin) (PubMed:11226248). Under conditions of apoptosis or calcium influx, cleaves CDH1 (PubMed:11953314). This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling (PubMed:11226248). Required for normal embryonic brain and skeleton development, and for normal angiogenesis (PubMed:9160754, PubMed:10421573, PubMed:12834865). Mediates the proteolytic cleavage of EphB2/CTF1 into EphB2/CTF2 (PubMed:17428795). The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is involved in calcium homeostasis (PubMed:16959576). Involved in the regulation of neurite outgrowth (By similarity). Is a regulator of presynaptic facilitation, spike transmission and synaptic vesicles replenishment in a process that depends on gamma-secretase activity. It acts through the control of SYT7 presynaptic expression (PubMed:30429473).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR203433 | Psen1 (Myc-DDK-tagged) - Mouse presenilin 1 (cDNA clone MGC:25288 IMAGE:4511140) |
CNY 2,400.00 |
|
| MR203433L3 | Lenti ORF clone of Psen1 (Myc-DDK-tagged) - Mouse presenilin 1 (cDNA clone MGC:25288 IMAGE:4511140) |
CNY 4,750.00 |
|
| MR203433L4 | Lenti ORF clone of Psen1 (mGFP-tagged) - Mouse presenilin 1 (cDNA clone MGC:25288 IMAGE:4511140) |
CNY 4,750.00 |
