Rchy1 (BC057143) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mARNIP, Pirh2, ARNIP, CHIMP, PRO1996
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203406 representing BC057143 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGACGGCGCGGGAAGATGGCGTCCGCAACCTGGCGCAGGGGCCGCGGGGCTGCGAGCACTATG ACAGAGCTTGTCTCCTCAAGGCACCTTGTTGTGACAAGCTTTATACCTGCCGGCTGTGTCACGATACCAA TGAAGATCATCAGCTAGATCGTTTCAAAGTCAAGGAAGTGCAGTGCATCAACTGTGAAAAACTCCAACAT GCCCAGCAGACTTGTGAAGACTGTAGCACATTGTTTGGAGAATATTACTGCAGTATTTGCCACCTGTTTG ACAAAGATAAGAGGCAGTACCACTGTGAGAGCTGTGGGATTTGTAGGATTGGTCCAAAGGAAGATTTTTT CCATTGCTTGAAATGTAATTTATGCCTAACCACGAATCTTCGAGGAAAACACAAGTGTATTGAAAATGTT TCCCGGCAGAATTGTCCAATATGCTTGGAGGACATTCACACCTCCCGTGTTGTTGCTCACGTCTTGCCGT GTGGGCATCTCCTACATAGAACGTGTTATGAAGAAATGTTGAAAGAAGGTTACAGATGCCCATTGTGTAT GCATTCTGCCTTAGACATGACTCGGTACTGGAGACAACTAGATACTGAGGTAGCACAGACTCCCATGCCA TCCGAATACCAGAACGTGACTGTTGACATTCTCTGCAATGACTGTAATGGACGATCCACAGTCCAGTTCC ATATCTTAGGCATGAAATGTAAGCTTTGTGACTCCTACAATACTGCCCAAGCTGGAGGCCGTAGAGTGCC AGTGGATCAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203406 representing BC057143 Red=Cloning site Green=Tags(s) MAATAREDGVRNLAQGPRGCEHYDRACLLKAPCCDKLYTCRLCHDTNEDHQLDRFKVKEVQCINCEKLQH AQQTCEDCSTLFGEYYCSICHLFDKDKRQYHCESCGICRIGPKEDFFHCLKCNLCLTTNLRGKHKCIENV SRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDTEVAQTPMP SEYQNVTVDILCNDCNGRSTVQFHILGMKCKLCDSYNTAQAGGRRVPVDQQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC057143
ORF Size	785 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC057143, AAH57143
RefSeq Size	1989 bp
RefSeq ORF	785 bp
Locus ID	68098
Gene Summary	This gene encodes a protein containing CHY-, CTCHY-, and RING-type zinc-fingers. The encoded protein functions as an E3 ubiquitin ligase, and mediates the degradation of target proteins such as p53. The activity of this protein is important in cell cycle regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

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