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Maf1 (NM_026859) Mouse Tagged ORF Clone
CAT＃: MG203339
- TrueORF®
Maf1 (tGFP-tagged) - Mouse MAF1 homolog (S. cerevisiae) (Maf1)
ORF Plasmid: DDK
"NM_026859" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110068E11Rik; AU042856 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203339 representing NM_026859
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTATTGGAGAACTCCAGCTTTGAGGCCATCAACTCACAGTTGACAGTGGAGACTGGAGATGCCC ATATTATTGGCAGGATTGAAAGCTACTCGTGTAAGATGGCGGGAGATGATAAACATATGTTCAAGCAGTT CTGCCAGGAGGGCCAGCCCCATGTGTTGGAGGCACTGTCCCCACCCCAAACTTCAGGCCTCAGTCCCAGC AGACTGAGCAAGAGCCAGGGTGGTGATGATGAGAGTCCTCTGAGCGACAAGTGCAGCCGCAAGACCCTCT TCTATCTGATTGCCACCCTCAATGAGTCCTTCCGGCCAGACTATGACTTCAGCACAGCCAGAAGTCATGA ATTCAGCCGAGAGCCAAGCCTCCGCTGGGTGGTAAATGCAGTCAACTGCAGCCTGTTTTCAGCTGTTCGT GAAGACTTCAAGGCCCTGAAGCCACAGCTGTGGAATGCAGTGGATGAGGAGATCTGCTTAGCTGAGTGTG ACATCTACAGCTATAACCCAGATCTAGACTCCGACCCCTTTGGGGAAGATGGAAGCCTCTGGTCATTCAA CTATTTCTTTTACAATAAGAGACTGAAGCGAATTGTCTTCTTTAGCTGCCGCTCCATCAGTGGCTCCACG TACACACCCTCAGAGGCAGGCAATGCACTGGACTTGGAACTGGGGGCAGAGGAGGCTGATGAAGAGAGTG GAGGTGGAGGCGGTGAGGGCAGGGCAGAGGAGACCAGCACCATGGAGGAAGACAGGGTTCCAGTGATCTG TATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203339 representing NM_026859
Red=Cloning site Green=Tags(s) MKLLENSSFEAINSQLTVETGDAHIIGRIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPS RLSKSQGGDDESPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLRWVVNAVNCSLFSAVR EDFKALKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCRSISGST YTPSEAGNALDLELGAEEADEESGGGGGEGRAEETSTMEEDRVPVICM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026859 |
| ORF Size | 774 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026859.2, NP_081135.2 |
| RefSeq Size | 1702 bp |
| RefSeq ORF | 777 bp |
| Locus ID | 68877 |
| UniProt ID | Q9D0U6 |
| Gene Summary | Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance (By similarity). Plays also a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation (PubMed:30110641). Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB. When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout. Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201311 | Maf1 (untagged) - Mouse MAF1 homolog (S. cerevisiae) (Maf1), transcript variant 2, (10ug) |
CNY 2,400.00 |
|
| MR203339 | Maf1 (Myc-DDK-tagged) - Mouse MAF1 homolog (S. cerevisiae) (Maf1), transcript variant 2 |
CNY 2,400.00 |
|
| MR203339L3 | Lenti ORF clone of Maf1 (Myc-DDK-tagged) - Mouse MAF1 homolog (S. cerevisiae) (Maf1), transcript variant 2 |
CNY 4,750.00 |
|
| MR203339L4 | Lenti ORF clone of Maf1 (mGFP-tagged) - Mouse MAF1 homolog (S. cerevisiae) (Maf1), transcript variant 2 |
CNY 4,750.00 |
