Fstl3 (BC061052) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	E030038F23Rik; Flrg
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203294 representing BC061052 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTTCTGGGGCACTGTGGCCGCTGCTTTGGGGAGCCCTGGTCTGGACAGTGGGATCCGTGGGCGCCG TGATGGGCTCCGAGGATTCTGTGCCCGGTGGCGTGTGCTGGCTCCAGCAGGGCAGAGAGGCCACCTGCAG TCTGGTGCTGAAGACTCGTGTCAGCCGGGAGGAGTGCTGTGCTTCCGGCAACATCAACACCGCCTGGTCC AACTTCACCCACCCAGGCAATAAAATCAGCCTGCTAGGGTTCCTGGGCCTCGTCCACTGCCTCCCCTGCA AAGATTCCTGCGACGGAGTGGAGTGCGGCCCCGGCAAGGCGTGCCGCATGCTGGGGGGCCGTCCACACTG CGAGTGCGTGCCCAACTGCGAGGGGCTTCCCGCGGGCTTCCAGGTCTGCGGCTCTGATGGCGCCACCTAC CGGGACGAATGCGAACTGCGCACCGCGCGCTGTCGCGGACACCCAGACTTGCGCGTCATGTACCGCGGCC GCTGTCAAAAGTCTTGCGCTCAGGTAGTGTGCCCGCGTCCCCAGTCGTGCCTTGTGGATCAGACCGGCAG CGCACACTGCGTGGTGTGTCGCGCTGCGCCCTGCCCAGTACCTTCCAACCCCGGCCAAGAACTCTGTGGC AACAACAACGTTACCTACATCTCGTCGTGTCACCTGCGCCAGGCCACTTGCTTCCTGGGCCGCTCCATTG GGGTTCGCCACCCAGGCATCTGCACAGGTGGCCCCAAAGTACCAGCAGAGGAGGAAGAGAACTTCGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203294 representing BC061052 Red=Cloning site Green=Tags(s) MRSGALWPLLWGALVWTVGSVGAVMGSEDSVPGGVCWLQQGREATCSLVLKTRVSREECCASGNINTAWS NFTHPGNKISLLGFLGLVHCLPCKDSCDGVECGPGKACRMLGGRPHCECVPNCEGLPAGFQVCGSDGATY RDECELRTARCRGHPDLRVMYRGRCQKSCAQVVCPRPQSCLVDQTGSAHCVVCRAAPCPVPSNPGQELCG NNNVTYISSCHLRQATCFLGRSIGVRHPGICTGGPKVPAEEEENFV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC061052
ORF Size	770 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC061052, AAH61052
RefSeq Size	1748 bp
RefSeq ORF	770 bp
Locus ID	83554
Gene Summary	The secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiation. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. The nuclear form is probably involved in transcriptional regulation via interaction with MLLT10 (By similarity).[UniProtKB/Swiss-Prot Function]

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