United States
Germany
Japan
United Kingdom
China
Tfam (NM_009360) Mouse Tagged ORF Clone
CAT＃: MG203015
- TrueORF®
Tfam (tGFP-tagged) - Mouse transcription factor A, mitochondrial (Tfam)
ORF Plasmid: DDK
"NM_009360" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI661103; Hmgts; mtTFA; tsHMG |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203015 representing NM_009360
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGTTCCGGGGAATGTGGAGCGTGCTAAAAGCACTGGGGCGCACGGGGGTCGAGATGTGCGCGG GCTGCGGGGGTCGCATCCCCTCGTCTATCAGTCTTGTCTGTATTCCGAAGTGTTTTTCCAGCATGGGTAG CTATCCAAAGAAACCTATGAGTTCATACCTTCGATTTTCCACAGAACAGCTACCCAAATTTAAAGCTAAA CACCCAGATGCAAAACTTTCAGAATTGGTTAGGAAAATTGCAGCCCTGTGGAGGGAGCTACCAGAAGCAG AAAAAAAGGTTTATGAAGCTGATTTTAAAGCTGAGTGGAAAGCATACAAAGAAGCTGTGAGCAAGTATAA AGAGCAGCTAACTCCAAGTCAGCTGATGGGTATGGAGAAGGAGGCCCGGCAGAGACGGTTAAAAAAGAAA GCACTGGTAAAGAGAAGAGAATTAATTTTGCTTGGAAAACCAAAAAGACCTCGTTCAGCATATAACATTT ATGTATCTGAAAGCTTCCAGGAGGCAAAGGATGATTCGGCTCAGGGAAAATTGAAGCTTGTAAATGAGGC TTGGAAAAATCTGTCTCCTGAGGAAAAGCAGGCATATATTCAGCTTGCTAAAGATGATAGGATTCGTTAC GACAATGAAATGAAGTCTTGGGAAGAGCAGATGGCTGAAGTTGGACGAAGTGATCTCATCCGTCGAAGTG TGAAACGATCCGGAGACATCTCTGAGCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203015 representing NM_009360
Red=Cloning site Green=Tags(s) MALFRGMWSVLKALGRTGVEMCAGCGGRIPSSISLVCIPKCFSSMGSYPKKPMSSYLRFSTEQLPKFKAK HPDAKLSELVRKIAALWRELPEAEKKVYEADFKAEWKAYKEAVSKYKEQLTPSQLMGMEKEARQRRLKKK ALVKRRELILLGKPKRPRSAYNIYVSESFQEAKDDSAQGKLKLVNEAWKNLSPEEKQAYIQLAKDDRIRY DNEMKSWEEQMAEVGRSDLIRRSVKRSGDISEH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009360 |
| ORF Size | 729 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009360.4 |
| RefSeq Size | 3920 bp |
| RefSeq ORF | 732 bp |
| Locus ID | 21780 |
| UniProt ID | P40630 |
| Gene Summary | Isoform Mitochondrial binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. Isoform Nuclear may also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202747 | Tfam (untagged) - Mouse transcription factor A, mitochondrial (Tfam), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MR203015 | Tfam (Myc-DDK-tagged) - Mouse transcription factor A, mitochondrial (Tfam), nuclear gene encoding mitochondrial protein |
CNY 2,081.00 |
|
| MR203015L3 | Lenti ORF clone of Tfam (Myc-DDK-tagged) - Mouse transcription factor A, mitochondrial (Tfam), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR203015L4 | Lenti ORF clone of Tfam (mGFP-tagged) - Mouse transcription factor A, mitochondrial (Tfam), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
