Apip (NM_019735) Mouse Tagged ORF Clone
CAT#: MG202974
- TrueORF®
Apip (tGFP-tagged) - Mouse APAF1 interacting protein (Apip)
ORF Plasmid: DDK
"NM_019735" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | APIP2; CGI-29; Mmrp19 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202974 representing NM_019735
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCTGTCAAGCTCAAGGAGACTGTTGCTCGCGGCCGTGTGGCGCGCAGGACAAGGAGCACCCCC GATTCCTGATCCCAGAACTTTGCAAACAGTTTTACCATCTGGGCTGGGTCACCGGCACTGGAGGGGGAAT CAGCTTGAAGCATGGCAATGAAATCTACATTGCTCCCTCAGGCGTGCAAAAGGAACGCATTCAGCCAGAA GACATGTTTGTGTGTGACATTAATGAGCAGGACATAAGCGGGCCTCCAGCATCTAAGAAGCTGAAAAAAA GCCAGTGCACTCCTCTTTTCATGAATGCTTATACCATGAGAGGAGCTGGCGCAGTGATTCATACCCACTC TAAAGCTGCTGTGATGGCTACCCTTCTGTTTCCAGGACAGGAGTTTAAAATTACACATCAAGAGATGATC AAAGGAATAAGGAAATGTACCTCAGGAGGCTATTACAGATACGATGATATGTTAGTGGTACCTATTATTG AGAACACTCCTGAAGAGAAGGATCTCAAAGAAAGGATGGCTCATGCCATGAACGAGTACCCAGACTCCTG TGCGGTTCTTGTCCGGCGTCATGGGGTGTACGTGTGGGGAGAAACATGGGAGAAAGCAAAAACCATGTGT GAGTGTTATGACTACCTGTTTGACATTGCTGTCTCCATGAAGAAGATGGGACTCGATCCAACACAGCTCC CAGTTGGAGAAAATGGAATTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202974 representing NM_019735
Red=Cloning site Green=Tags(s) MSGCQAQGDCCSRPCGAQDKEHPRFLIPELCKQFYHLGWVTGTGGGISLKHGNEIYIAPSGVQKERIQPE DMFVCDINEQDISGPPASKKLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLFPGQEFKITHQEMI KGIRKCTSGGYYRYDDMLVVPIIENTPEEKDLKERMAHAMNEYPDSCAVLVRRHGVYVWGETWEKAKTMC ECYDYLFDIAVSMKKMGLDPTQLPVGENGIV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019735 |
ORF Size | 723 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019735.4, NP_062709.3 |
RefSeq Size | 897 bp |
RefSeq ORF | 726 bp |
Locus ID | 56369 |
UniProt ID | Q9WVQ5 |
Gene Summary | Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210045 | Apip (untagged) - Mouse APAF1 interacting protein (Apip), (10ug) |
CNY 3,990.00 |
|
MR202974 | Apip (Myc-DDK-tagged) - Mouse APAF1 interacting protein (Apip) |
CNY 2,400.00 |
|
MR202974L3 | Lenti ORF clone of Apip (Myc-DDK-tagged) - Mouse APAF1 interacting protein (Apip) |
CNY 4,750.00 |
|
MR202974L4 | Lenti ORF clone of Apip (mGFP-tagged) - Mouse APAF1 interacting protein (Apip) |
CNY 4,750.00 |