Gar1 (NM_026578) Mouse Tagged ORF Clone
CAT#: MG202791
- TrueORF®
Gar1 (tGFP-tagged) - Mouse nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs) (Nola1)
ORF Plasmid: DDK
"NM_026578" in other vectors (2)
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CNY 2,470.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AA409823; AI326794; C430047J18Rik; Nola1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202791 representing NM_026578
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTCCGAGGCGGAGGTCGCGGAGGCTTTAATCGCGGTGGTGGAGGCGGAGGCTTCAACCGTGGCG GCGGCAGCAACAACCACTTCCGAGGGGGCGGCGGAGGCGGCGGCGGCAGTTTCAGGGGCGGAGGCGGCGG CGGCGGCGGCAGTTTCAGGGGCGGCGGCCGAGGAGGATTTGGACGAGGGGGCGGTCGTGGAGGCTTTAAT AAATTTCAAGATCAAGGGCCTCCAGAACGTGTCGTCTTGTTAGGAGAATTCATGCATCCCTGTGAAGATG ACATCGTGTGTAAATGTACCACCGAGGAGAACAAGGTGCCCTACTTCAACGCCCCTGTTTACTTAGAAAA CAAAGAGCAAGTCGGGAAAGTGGATGAGATATTTGGACAGCTTAGAGATTTTTATTTTTCAGTTAAGTTG TCAGAAAACATGAAGGCATCTTCCTTTAAAAAGCTACAGAAGTTCTATATAGACCCATACAAGCTGCTGC CGCTGCAGAGGTTTCTGCCTCGTCCTCCTGGTGAGAAAGGACCTCCCAGAGGTGGCGGCGGTGGCGGCAG GGGAGGTCGAGGAGGAGGAAGAGGAGGCGGTGGCCGAGGTGGTGGAAGAGGTGGTGGTTTTAGAGGAGGC AGAGGAGGAGGTGGGGGCTTCAGAGGAGGAAGAGGAGGTGGCGGATTCCGAGGAAGGGGACAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202791 representing NM_026578
Red=Cloning site Green=Tags(s) MSFRGGGRGGFNRGGGGGGFNRGGGSNNHFRGGGGGGGGSFRGGGGGGGGSFRGGGRGGFGRGGGRGGFN KFQDQGPPERVVLLGEFMHPCEDDIVCKCTTEENKVPYFNAPVYLENKEQVGKVDEIFGQLRDFYFSVKL SENMKASSFKKLQKFYIDPYKLLPLQRFLPRPPGEKGPPRGGGGGGRGGRGGGRGGGGRGGGRGGGFRGG RGGGGGFRGGRGGGGFRGRGH TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_026578 |
ORF Size | 693 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026578.3, NP_080854.1 |
RefSeq Size | 1271 bp |
RefSeq ORF | 696 bp |
Locus ID | 68147 |
UniProt ID | Q9CY66 |
Gene Summary | Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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FAQs |
SDS |