Mmachc (NM_025962) Mouse Tagged ORF Clone
CAT#: MG202551
- TrueORF®
Mmachc (tGFP-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc)
ORF Plasmid: DDK
"NM_025962" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1810037K07Rik; CblC |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202551 representing NM_025962
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGACAGAGCCCTCAAACCCTTCTTAAAGAGCTGCCACTTCCAAACACTGAGAGACCCGGTGGATC AATGTGTGTCCTACCACCTGAGGAGTGTTACAGAGAAGTTTCCAGAAGTGCATATGGAAGTCATTGCTGA CTATGAGGTACACCCCAATCGGCGACCTAAGATTCTCGCCCAGACAGCAGCCCATGTGGCAGGTGCTGCT TATTACTACCAACGACAAGATGTGGATGCAGACCCATGGGGGACCCAGCACATAGCAGGTGTGTGCATAC ACCCCCGATTTGGGGGCTGGTTTGCCATCCGAGGGGTTATGTTGCTGCCAGGGATTGAAGTGCCAAATTT GCCACCCAGAAAGCCCCCTGACTGTGTGCCTACAAGAGCTGGCCGCATCACTCTGCTTGAAGGTTTCAAT TTCCATTGGCGGGACTGGACTTACCGTGATGCTGTGACTCCTGAAGAACGGTACTCCGAAGAACAGAAGA TCTACTTTTCCACCCCACCTGCCCAACGCTTGGCCCTATTAGGCTTAGCCCAACCCTCAGAACACCCTAG CACTACATCTGAGCTTCCTCTTTCCTTGCTTACTAAACCTCAGAATTCCAGGAGAGCTCGAAGCTGGCTG AGTCCAAGTGTCTCACCACCTGTATCCCCAGGCCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202551 representing NM_025962
Red=Cloning site Green=Tags(s) MFDRALKPFLKSCHFQTLRDPVDQCVSYHLRSVTEKFPEVHMEVIADYEVHPNRRPKILAQTAAHVAGAA YYYQRQDVDADPWGTQHIAGVCIHPRFGGWFAIRGVMLLPGIEVPNLPPRKPPDCVPTRAGRITLLEGFN FHWRDWTYRDAVTPEERYSEEQKIYFSTPPAQRLALLGLAQPSEHPSTTSELPLSLLTKPQNSRRARSWL SPSVSPPVSPGP TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025962 |
ORF Size | 666 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025962.2 |
RefSeq Size | 1863 bp |
RefSeq ORF | 840 bp |
Locus ID | 67096 |
UniProt ID | Q9CZD0 |
Gene Summary | Catalyzes the reductive dealkylation of cyanocobalamin to cob(II)alamin, using FAD or FMN as cofactor and NADPH as cosubstrate. Can also catalyze the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. Under anaerobic conditions cob(I)alamin is the first product; it is highly reactive and is converted to aquocob(II)alamin in the presence of oxygen. Binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202568 | Mmachc (untagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc), (10ug) |
CNY 2,400.00 |
|
MR202551 | Mmachc (Myc-DDK-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc) |
CNY 2,400.00 |
|
MR202551L3 | Lenti ORF clone of Mmachc (Myc-DDK-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc) |
CNY 4,750.00 |
|
MR202551L4 | Lenti ORF clone of Mmachc (mGFP-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc) |
CNY 4,750.00 |