Pigf (NM_008838) Mouse Tagged ORF Clone
CAT#: MG202476
- TrueORF®
Pigf (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class F (Pigf)
ORF Plasmid: DDK
"NM_008838" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202476 representing NM_008838
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGATACTGACATCAAGAGACTTCTGTATACCAATCTTTTGTGCGTATTTTCAATTTTCCTAAGCA TTTTCATTCCGTCCTTCTTCGTGGACAACTTCTCAGTATTGGAAGCTCATTTGACCTGGCTGTGTATCTG CTCTGCTTCTGTGACCACTGTCAATCTCTTGTCGTACTTAGTAGTGAAACCGAATGTGTCCTCTAAAAGA AGTTCATTGTCACATAAGGTAACGCGAGCTTTGAAATGCTGTGTCTTCTTCCTTATGTCTTGTTTTCTCC TTCACATCATTTTTGTTCTATATGGAGCACCACTAATCGAGTTGGTGCTGGAAACGTTTTTATTTGCAGT TGTTTTGTCTACTTTTACTACAGTACCTTGTCTCTGTTTGTTAGGACCAAACCTCAAAGCATGGCTGAGA GTTTTCAGTAGAAATGGAGTTACATCTATTTGGGAGAATAGTCTTCAGATCACTACAATTTCTAGTTTCA CAGGAGCGTGGCTTGGAGCATTTCCTATTCCACTGGATTGGGAAAGACCGTGGCAGGTGTGGCCCATCTC CTGTACACTCGGAGCGACCTTTGGCTACGTGGCTGGCCTGGTTATCTCGCCACTCTGGATCTACTGGAAT AGAAAGCAGCTTACATACAAGAACAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202476 representing NM_008838
Red=Cloning site Green=Tags(s) MKDTDIKRLLYTNLLCVFSIFLSIFIPSFFVDNFSVLEAHLTWLCICSASVTTVNLLSYLVVKPNVSSKR SSLSHKVTRALKCCVFFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPNLKAWLR VFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGATFGYVAGLVISPLWIYWN RKQLTYKNN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008838 |
ORF Size | 657 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008838.1, NP_032864.1 |
RefSeq Size | 980 bp |
RefSeq ORF | 660 bp |
Locus ID | 18701 |
UniProt ID | O09101 |
Gene Summary | Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204558 | Pigf (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class F (Pigf), (10ug) |
CNY 2,400.00 |
|
MR202476 | Pigf (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class F (Pigf) |
CNY 2,400.00 |
|
MR202476L3 | Lenti ORF clone of Pigf (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class F (Pigf) |
CNY 4,750.00 |
|
MR202476L4 | Lenti ORF clone of Pigf (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class F (Pigf) |
CNY 4,750.00 |