Clta (BC057660) Mouse Tagged ORF Clone

CAT#: MG202459

  • TrueORF®

Clta (tGFP-tagged) - Mouse clathrin, light polypeptide (Lca) (cDNA clone MGC:68133 IMAGE:3154277)



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Mouse Protein / Lysate Request

CNY 2,380.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AV026556; Lca
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG202459 representing BC057660
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGTTGGATCCATTCGGCGCTCCCGCCGGTGCGCCCGGTGGCCCCGCTCTAGGAAACGGGGTGG
CGGGCGCCGGCGAAGAAGACCCGGCCGCGGCCTTCTTGGCGCAGCAAGAGAGTGAGATTGCGGGCATCGA
GAACGATGAGGCCTTCGCCATCCTGGACGGCGGCGCCCCCGGGCCGCAGCCACACGGCGAGCCGCCGGGA
GGTCCGGATGCTGTTGACGGAGTGATGAATGGTGAATACTACCAGGAGAGCAATGGTCCAACAGACAGTT
ACGCAGCCATTTCAGAAGTGGATCGATTGCAATCAGAGCCTGAAAGTATCCGTAAGTGGAGAGAAGAGCA
GACAGAGCGCCTGGAAGCCCTCGATGCCAATTCTCGGAAGCAAGAAGCGGAGTGGAAAGAAAAGGCGATA
AAGGAGCTGGAAGAGTGGTATGCGAGGCAGGACGAGCAGCTACAGAAGACAAAGGCAAACAACAGGGCAG
CAGAAGAAGCCTTTGTAAATGACATTGACGAGTCATCCCCAGGCACTGAGTGGGAGCGGGTGGCCCGCCT
GTGTGACTTTAACCCCAAGTCCAGCAAACAGGCCAAAGATGTCTCTCGCATGCGCTCAGTCCTCATCTCC
CTGAAGCAGGCCCCCCTGGTGCAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG202459 representing BC057660
Red=Cloning site Green=Tags(s)

MAELDPFGAPAGAPGGPALGNGVAGAGEEDPAAAFLAQQESEIAGIENDEAFAILDGGAPGPQPHGEPPG
GPDAVDGVMNGEYYQESNGPTDSYAAISEVDRLQSEPESIRKWREEQTERLEALDANSRKQEAEWKEKAI
KELEEWYARQDEQLQKTKANNRAAEEAFVNDIDESSPGTEWERVARLCDFNPKSSKQAKDVSRMRSVLIS
LKQAPLVH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC057660
ORF Size 654 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC057660, AAH57660
RefSeq Size 1058 bp
RefSeq ORF 656 bp
Locus ID 12757
Gene Summary Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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