Hprt (NM_013556) Mouse Tagged ORF Clone
CAT#: MG202453
- TrueORF®
Hprt (tGFP-tagged) - Mouse hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1)
ORF Plasmid: DDK
"NM_013556" in other vectors (4)
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CNY 5,200.00
Cited in 1 publication. |
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | C81579; HPGRT; Hpr; Hprt1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202453 representing NM_013556
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCCGCAGTCCCAGCGTCGTGATTAGCGATGATGAACCAGGTTATGACCTAGATTTGTTTTGTA TACCTAATCATTATGCCGAGGATTTGGAAAAAGTGTTTATTCCTCATGGACTGATTATGGACAGGACTGA AAGACTTGCTCGAGATGTCATGAAGGAGATGGGAGGCCATCACATTGTGGCCCTCTGTGTGCTCAAGGGG GGCTATAAGTTCTTTGCTGACCTGCTGGATTACATTAAAGCACTGAATAGAAATAGTGATAGATCCATTC CTATGACTGTAGATTTTATCAGACTGAAGAGCTACTGTAATGATCAGTCAACGGGGGACATAAAAGTTAT TGGTGGAGATGATCTCTCAACTTTAACTGGAAAGAATGTCTTGATTGTTGAAGATATAATTGACACTGGT AAAACAATGCAAACTTTGCTTTCCCTGGTTAAGCAGTACAGCCCCAAAATGGTTAAGGTTGCAAGCTTGC TGGTGAAAAGGACCTCTCGAAGTGTTGGATACAGGCCAGACTTTGTTGGATTTGAAATTCCAGACAAGTT TGTTGTTGGATATGCCCTTGACTATAATGAGTACTTCAGGGATTTGAATCACGTTTGTGTCATTAGTGAA ACTGGAAAAGCCAAATACAAAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202453 representing NM_013556
Red=Cloning site Green=Tags(s) MATRSPSVVISDDEPGYDLDLFCIPNHYAEDLEKVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKG GYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTG KTMQTLLSLVKQYSPKMVKVASLLVKRTSRSVGYRPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISE TGKAKYKA TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013556 |
ORF Size | 654 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013556.2, NP_038584.2 |
RefSeq Size | 1349 bp |
RefSeq ORF | 657 bp |
Locus ID | 15452 |
UniProt ID | P00493 |
Gene Summary | The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. [provided by RefSeq, Sep 2015] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Genome-wide genetic screening with chemically mutagenized haploid embryonic stem cells
,Forment, JV;Herzog, M;Coates, J;Konopka, T;Gapp, BV;Nijman, SM;Adams, DJ;Keane, TM;Jackson, SP;,
Nat. Chem. Biol.
,PubMed ID 27820796
[HPRT]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200159 | Hprt (untagged) - Mouse hypoxanthine guanine phosphoribosyl transferase (Hprt), (10ug) |
CNY 3,600.00 |
|
MR202453 | Hprt (Myc-DDK-tagged) - Mouse hypoxanthine guanine phosphoribosyl transferase (Hprt) |
CNY 3,600.00 |
|
MR202453L3 | Lenti ORF clone of Hprt (Myc-DDK-tagged) - Mouse hypoxanthine guanine phosphoribosyl transferase (Hprt) |
CNY 5,890.00 |
|
MR202453L4 | Lenti ORF clone of Hprt (mGFP-tagged) - Mouse hypoxanthine guanine phosphoribosyl transferase (Hprt) |
CNY 5,890.00 |