Rab11b (BC054753) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	A730055L17Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG202439 representing BC054753 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACCCGGGACGACGAGTACGATTACCTATTCAAAGTGGTGCTTATTGGGGACTCAGGTGTAGGTA AGAGCAACCTGCTGTCACGCTTCACCAGAAACGAATTCAACCTAGAGAGCAAGAGTACCATCGGAGTGGA GTTCGCCACTCGCAGCATTCAGGTGGACGGCAAGACCATCAAGGCTCAGATCTGGGACACTGCTGGCCAG GAGCGCTACCGTGCCATTACCTCTGCGTACTACCGTGGTGCAGTGGGTGCACTGCTGGTATATGACATTG CCAAGCACTTGACATATGAGAACGTGGAGCGCTGGCTGAAGGAGCTGCGGGATCATGCAGATAGCAACAT TGTCATCATGCTGGTGGGCAACAAGAGTGACCTGCGCCACCTTCGGGCTGTGCCCACTGATGAGGCCCGT GCCTTTGCAGAAAAGAACAACTTGTCCTTCATTGAGACCTCAGCCTTGGATTCCACCAATGTAGAGGAAG CATTCAAGAACATCCTCACAGAAATCTACCGTATTGTGTCACAGAAGCAAATCGCTGACCGTGCAGCCCA CGATGAGTCCCCTGGCAACAACGTGGTGGACATCAGTGTGCCACCCACCACCGATGGACAGAGACCCAAC AAGCTGCAGTGCTGCCAGAGCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG202439 representing BC054753 Red=Cloning site Green=Tags(s) MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQ ERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEAR AFAEKNNLSFIETSALDSTNVEEAFKNILTEIYRIVSQKQIADRAAHDESPGNNVVDISVPPTTDGQRPN KLQCCQSL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC054753
ORF Size	656 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC054753, AAH54753
RefSeq Size	1788 bp
RefSeq ORF	656 bp
Locus ID	19326
Gene Summary	The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11B plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis.[UniProtKB/Swiss-Prot Function]

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