Naca (BC083340) Mouse Tagged ORF Clone
CAT#: MG202376
- TrueORF®
Naca (tGFP-tagged) - Mouse nascent polypeptide-associated complex alpha polypeptide (cDNA clone MGC:102085 IMAGE:5700166), complete
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CNY 2,380.00
货期*
3周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | skNAC, mKIAA0363 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202376 representing BC083340
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGTGAAGCCACAGAAACCGTCCCTGCTACAGAGCAGGAGTTGCCACAGCCTCAGGCTGAGACAG GATCGGGAACAGAGTCTGACAGTGATGAGTCAGTACCAGAGCTCGAGGAACAAGACTCCACACAGACGGC CACGCAGCAAGCCCAGCTGGCAGCCGCAGCAGAGATCGATGAAGAACCTGTTAGTAAAGCCAAGCAGAGT CGAAGTGAGAAGAAGGCAAGGAAGGCTATGTCCAAACTGGGTCTTCGACAGGTTACAGGGGTTACGAGAG TCACTATCCGAAAATCTAAAAATATCCTCTTTGTCATCACAAAACCCGATGTCTACAAGAGCCCAGCTTC AGACACCTACATAGTGTTTGGGGAAGCCAAGATTGAAGATTTGTCTCAGCAAGCACAGTTAGCAGCTGCT GAGAAATTCAAAGTTCAAGGTGAAGCTGTTTCAAACATTCAGGAAAACACTCAGACTCCAACCGTCCAAG AGGAGAGTGAAGAAGAGGAGGTTGATGAGACGGGTGTGGAAGTTAAGGACATAGAACTGGTCATGTCGCA AGCAAACGTATCAAGAGCAAAGGCTGTTCGAGCCCTGAAAAACAACAGTAATGATATTGTAAATGCTATT ATGGAATTAACAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202376 representing BC083340
Red=Cloning site Green=Tags(s) MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQTATQQAQLAAAAEIDEEPVSKAKQS RSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAA EKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAI MELTM TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC083340 |
ORF Size | 647 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC083340, AAH83340 |
RefSeq Size | 819 bp |
RefSeq ORF | 647 bp |
Locus ID | 17938 |
Gene Summary | Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites) (By similarity). Isoform 1 and isoform 2 appear to bind DNA and play roles in transcription. Isoform 1 may function as a specific coactivator for JUN, acting to stabilize the interaction of JUN homodimers with promoter elements.[UniProtKB/Swiss-Prot Function] |
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