Gemin8 (BC023488) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC32253
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG202283 representing BC023488 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTGGATGCAGGGCCACCAGAATGCCTACAGAAAGTTCAGGGATTCCTATTTCACATCCCCGTGGC TCTTCCCTCATGGAGCTCTTCCCTGGAACTCTCCTGCTTATGAGGCTGGGCATCCTTGGGACTCTCAAGG CCAGCACATGGCGCAGCAGGAGTCTCCCTACCGTGTTTCCCATCCCAAAAGCCCTGGGCAGCCTCTGCAT AATAGCAGTAGAACCCAGGCATCCACAAGAGGGAATGAAGCACGGTGTGAGGAGGAAGAGCTGGAGTCAG ATTCAGATGATGAAGTAGAGTGCGACCTGAGCAATATGGAGATCACCGAGGAGCTCCGGCAGTACTTCGC GCAGACCGAGAGGCACAGAGAGGAGAGAAGGCGACAGCAGCAGCTGGACGCAGAGCGCCTGAATTACTAT GTGAATGCGGACCATGGCCTGTACTTCAACCACCGTAGGTCACTGGAACCCCCATCTGAGAAGCCCTGGG AGCGGCGCCAAGCAGAGATGAAGCGCTTATATGGCAACAGTGCTCCCAAGATCCTGGCCATGGAGACTGC TGTACAGCTGAGCTTTGACAAGCACTGTGACAGAAAGCAACCAAAGTACTGGCCTGTCATTCCCCTGAAG TTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG202283 representing BC023488 Red=Cloning site Green=Tags(s) MLWMQGHQNAYRKFRDSYFTSPWLFPHGALPWNSPAYEAGHPWDSQGQHMAQQESPYRVSHPKSPGQPLH NSSRTQASTRGNEARCEEEELESDSDDEVECDLSNMEITEELRQYFAQTERHREERRRQQQLDAERLNYY VNADHGLYFNHRRSLEPPSEKPWERRQAEMKRLYGNSAPKILAMETAVQLSFDKHCDRKQPKYWPVIPLK F TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC023488
ORF Size	635 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC023488, AAH23488
RefSeq Size	1136 bp
RefSeq ORF	635 bp
Locus ID	237221
Gene Summary	The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus (By similarity).[UniProtKB/Swiss-Prot Function]

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