Dtd1 (BC026537) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	0610006H08Rik; DUE-B; Hars2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG202251 representing BC026537 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCCGTGGTGCAGCGCGTCACTCGGGCTAGCGTCACAGTTGGAGGAGAGCAGATAAGCGCCATCG GACGGGGCATCTGTGTCTTGCTGGGTATTTCCATGGAAGACTCACAGAAGGAACTGGAACACATGGTTCG AAAGATTTTAAATCTGCGTGTGTTTGAGGACGAGAGTGGGAAGCACTGGTCCAAGAGCGTGATGGATAAG GAGTACGAGGTGCTGTGTGTCAGCCAGTTCACCCTCCAGTGCGTCCTCAAGGGGAACAAGCCCGACTTCC ACCTGGCCATGCCCACAGAGCAGGCAGAGAGCTTCTACAACAGCTTCCTGGAGCAGCTTCGCAAGAGCTA CAGACCAGAGCTTATCAGAGATGGCAAGTTTGGTGCCTACATGCAGGTGCACATTCAGAACGATGGGCCT GTGACTATAGAGCTGGAGTCCCCTGCTCCTGGAGCTGCTAGCTCTGACCCAAAGCAGCTGTCAAAGCTGG AGAAACAGCAGCAGAGAAAAGAAAAGACCAGAGCCAAAGGCCCTTCTGAGTCAAGCAAAGAACGGAATGC ACCCCGCAAAGAAGACCGCAGCGCCAGCAGCGGGGCAGAGGGTGACGTGTCTTCTGAGCGGGAGCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG202251 representing BC026537 Red=Cloning site Green=Tags(s) MKAVVQRVTRASVTVGGEQISAIGRGICVLLGISMEDSQKELEHMVRKILNLRVFEDESGKHWSKSVMDK EYEVLCVSQFTLQCVLKGNKPDFHLAMPTEQAESFYNSFLEQLRKSYRPELIRDGKFGAYMQVHIQNDGP VTIELESPAPGAASSDPKQLSKLEKQQQRKEKTRAKGPSESSKERNAPRKEDRSASSGAEGDVSSEREP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC026537
ORF Size	629 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC026537, AAH26537
RefSeq Size	1239 bp
RefSeq ORF	629 bp
Locus ID	66044
Gene Summary	An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.[UniProtKB/Swiss-Prot Function]

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