Cldn10 (BC021770) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	6720456I16Rik, D14Ertd728e
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG201866 representing BC021770 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGGGCACAGATCTCAGCTCTGGTGTGTGGTGTTGGAGGGTTTGGTGCTCTCGTCGCTGCCACCA CATCCAACGAATGGAAAGTGACCACCCGAGCGTCGTCTGTGATTACCGCCACCTGGGTTTACCAGGGTCT GTGGATGAACTGCGCAGGTAACGCTCTGGGCTCCTTCCACTGCCGGCCACATTTCACTATCTTCAAAGTA GAAGGTTACATCCAGGCATGTAGAGGACTAATGATCGCTGCGGTCAGCCTGGGATTTTTCGGTTCCATTT TTGCACTCTTTGGAATGAAATGTACCAAAGTCGGAGGCTCAGATCAAGCCAAAGCTAAAATCGCTTGCTT GGCCGGGATTGTATTCATATTGTCAGGTCTGTGTTCCATGACAGGCTGTTCCCTGTATGCAAACAAAATC ACAACAGAATTCTTTGATCCTCTCTATATGGAGCAAAAAATGGGCTACACATACAACGGACCCACGTCTG TCATGTCTTCTCGGACCAAGTATCAAGGCGGAGAAGGAGATTTTAAAACCGCAGGCCCTTCAAAACAGTT TGATAAAAATGCCTATGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG201866 representing BC021770 Red=Cloning site Green=Tags(s) MSRAQISALVCGVGGFGALVAATTSNEWKVTTRASSVITATWVYQGLWMNCAGNALGSFHCRPHFTIFKV EGYIQACRGLMIAAVSLGFFGSIFALFGMKCTKVGGSDQAKAKIACLAGIVFILSGLCSMTGCSLYANKI TTEFFDPLYMEQKMGYTYNGPTSVMSSRTKYQGGEGDFKTAGPSKQFDKNAYV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC021770
ORF Size	581 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021770, AAH21770
RefSeq Size	1054 bp
RefSeq ORF	581 bp
Locus ID	58187
Gene Summary	This intronless gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight unction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Six alternatively spliced transcript variants have been identified, which encode different isoforms with distinct electric charge of the first extracellular loop and with or without the fourth transmembrane region. These isoforms exhibit distinct localization and function in paracellular anion or cation permeability. [provided by RefSeq, Aug 2010]

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