Mocs2 (BC024371) Mouse Tagged ORF Clone
CAT#: MG201790
- TrueORF®
Mocs2 (tGFP-tagged) - Mouse molybdenum cofactor synthesis 2 (cDNA clone MGC:35997 IMAGE:4983231)
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CNY 2,090.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI415403 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201790 representing BC024371
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAGCTTGGAGATCAGCAACTCCTGCTTCAGCCCGGAGACGAGGTCGCCATCATCCCGCCAATCAG TGGAGGATAATGCATCTGAGCCTTCTGGGAAAGATGTGGACGATGTCCAGGAGAAACCTAAAGACATAAT ACAGTTCACTGCCGAGAAGCTCTCTGTGGGGGAAGTGTCACAGTTGGTGGTGTCCCCTCTGTGTGGTGCA GTGTCTCTCTTTGTAGGGACTACAAGAAATAACTTTGAAGGCAAGAAAGTCATTAGCTTAGAATATGAAG CGTATGTACCGATGGCAGAAAATGAAATCAGAAAAATTTGTAATGACATTAGACAGAAATGGCCCGTGAG ACACATAGCAGTATTCCATCGGCTTGGTTTGGTTCCAGTGTCAGAAGCAAGCACAGTTATTGCTGTGTCT TCAGCTCACAGAGCCGCGTCCCTCGAAGCCGTGAGCTACGCCATTGATTCTTTAAAAGCCAAGGTGCCCA TATGGAAAAAGGAAATATATGAAGAATCAACCTCATCTTGGAAAAGAAACAAAGAGTGCTTCTGGGCAGC TGGTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201790 representing BC024371
Red=Cloning site Green=Tags(s) MSSLEISNSCFSPETRSPSSRQSVEDNASEPSGKDVDDVQEKPKDIIQFTAEKLSVGEVSQLVVSPLCGA VSLFVGTTRNNFEGKKVISLEYEAYVPMAENEIRKICNDIRQKWPVRHIAVFHRLGLVPVSEASTVIAVS SAHRAASLEAVSYAIDSLKAKVPIWKKEIYEESTSSWKRNKECFWAAGD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC024371 |
ORF Size | 569 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC024371, AAH24371 |
RefSeq Size | 1177 bp |
RefSeq ORF | 569 bp |
Locus ID | 17434 |
Gene Summary | Eukaryotic molybdoenzymes use a unique molybdenum cofactor (MoCo) consisting of a pterin, termed molybdopterin, and the catalytically active metal molybdenum. MoCo is synthesized from precursor Z by the heterodimeric enzyme molybdopterin synthase. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. The proteins were initially thought to be encoded from a bicistronic transcript. Based on experiments with the human molybdopterin synthase ortholog, they are now thought to be encoded from monocistronic transcripts. Alternatively spliced transcripts have been found for this locus that encode the large and small subunits. [provided by RefSeq, Jul 2008] |
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