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Tifa (NM_145133) Mouse Tagged ORF Clone
CAT＃: MG201692
- TrueORF®
Tifa (tGFP-tagged) - Mouse Traf2 binding protein (T2bp)
ORF Plasmid: DDK
"NM_145133" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | T2bp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG201692 representing NM_145133
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACCTTTGAAGACGCTGATACAGAGGAGACGGTCACTTGTCTCCAGATGACCATTTACCATCCTG GCCAACAAAGTGGGATATTTAAATCAATAAGGTTTTGCAGCAAAGAGAAGTTTCCTTCCATTGAAGTGGT GAAATTTGGACGCAATTCCAACATGTGCCAGTATACGTTTCAGGACAAACAGGTGTCCCGAATTCAGTTT GTTTTACAGCCGTTTAAACAGTTCAACAGCTCCGTTCTCTCGTTTGAAATAAAAAACATGAGCAAGAAAA CCAGTTTGATGGTAGACAACCAGGAGCTCGGCTACCTCAATAAAATGGACCTGCCTTACAAGTGTATGCT CAGGTTCGGAGAGTATCAGTTCCTGTTGCAGAAGGAAGACGGAGAGTCGGTGGAATCTTTTGAGACTCAA TTTATCATGTCTTCAAGACCTCTCTTGCAAGAAAACAACTGGCCAACACAGAATCCCATACCAGAGGATG GGATGTATTCTTCATACTTCACCCACAGAAGTTCTCCTTCAGAAATGGATGAAAACGAACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201692 representing NM_145133
Red=Cloning site Green=Tags(s) MSTFEDADTEETVTCLQMTIYHPGQQSGIFKSIRFCSKEKFPSIEVVKFGRNSNMCQYTFQDKQVSRIQF VLQPFKQFNSSVLSFEIKNMSKKTSLMVDNQELGYLNKMDLPYKCMLRFGEYQFLLQKEDGESVESFETQ FIMSSRPLLQENNWPTQNPIPEDGMYSSYFTHRSSPSEMDENEL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145133 |
| ORF Size | 552 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145133.4 |
| RefSeq Size | 2151 bp |
| RefSeq ORF | 555 bp |
| Locus ID | 211550 |
| UniProt ID | Q793I8 |
| Gene Summary | Adapter molecule that plays a key role in the activation of proinflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs) (PubMed:11798190). Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism (By similarity). TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria: ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of proinflammatory NF-kappa-B signaling (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207861 | Tifa (untagged) - Mouse TRAF-interacting protein with forkhead-associated domain (Tifa), (10ug) |
CNY 3,990.00 |
|
| MR201692 | Tifa (Myc-DDK-tagged) - Mouse TRAF-interacting protein with forkhead-associated domain (Tifa) |
CNY 2,400.00 |
|
| MR201692L3 | Lenti ORF clone of Tifa (Myc-DDK-tagged) - Mouse TRAF-interacting protein with forkhead-associated domain (Tifa) |
CNY 4,750.00 |
|
| MR201692L4 | Lenti ORF clone of Tifa (mGFP-tagged) - Mouse TRAF-interacting protein with forkhead-associated domain (Tifa) |
CNY 4,750.00 |
