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Rap1a (NM_145541) Mouse Tagged ORF Clone
CAT＃: MG201691
- TrueORF®
Rap1a (tGFP-tagged) - Mouse RAS-related protein-1a (Rap1a)
ORF Plasmid: DDK
"NM_145541" in other vectors (3)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI848598; G-22K; Krev-1; Rap1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG201691 representing NM_145541
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTGAGTACAAGCTAGTAGTCCTTGGTTCAGGAGGCGTGGGGAAGTCTGCTCTGACAGTTCAGTTTG TTCAGGGGATTTTTGTTGAAAAATATGACCCAACGATAGAAGATTCCTACAGAAAGCAAGTCGAGGTAGA TTGCCAACAGTGCATGCTGGAGATCCTGGACACTGCAGGAACCGAGCAATTTACAGCAATGAGGGATTTG TATATGAAGAATGGCCAAGGGTTTGCACTAGTTTATTCAATTACAGCTCAGTCTACGTTTAATGATTTAC AAGACTTGAGAGAACAGATTTTACGGGTTAAAGACACAGAAGATGTTCCAATGATTTTGGTTGGCAATAA ATGTGACTTGGAAGATGAACGGGTAGTTGGCAAAGAACAAGGCCAGAATTTAGCAAGACAGTGGTGTAAC TGTGCCTTTTTAGAATCTTCTGCAAAGTCAAAGATCAACGTTAATGAGATATTTTATGACCTGGTCAGAC AGATAAATAGAAAAACACCAGTGGAAAAGAAGAAGCCTAAAAAGAAATCATGTTTGCTGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201691 representing NM_145541
Red=Cloning site Green=Tags(s) MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDL YMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCN CAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145541 |
| ORF Size | 2471 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145541.5, NP_663516.1 |
| RefSeq Size | 1424 bp |
| RefSeq ORF | 555 bp |
| Locus ID | 109905 |
| UniProt ID | P62835 |
| Gene Summary | Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (By similarity). Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR201691 | Rap1a (Myc-DDK-tagged) - Mouse RAS-related protein-1a (Rap1a) |
CNY 2,400.00 |
|
| MR201691L3 | Lenti ORF clone of Rap1a (Myc-DDK-tagged) - Mouse RAS-related protein-1a (Rap1a) |
CNY 4,750.00 |
|
| MR201691L4 | Lenti ORF clone of Rap1a (mGFP-tagged) - Mouse RAS-related protein-1a (Rap1a) |
CNY 4,750.00 |
