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Selenot (NM_001040396) Mouse Tagged ORF Clone
CAT＃: MG201303
- TrueORF®
2810407C02Rik (GFP-tagged) - Mouse RIKEN cDNA 2810407C02 gene (2810407C02Rik), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)
ORF Plasmid: DDK
"NM_001040396" in other vectors (2)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged ORF Clone |
| Synonyms | 2810407C02Rik; 5730408P04Rik; Se; Selt |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG201303 representing NM_001040396
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTCCTGCTGCTTCTGCTGGTGGCGGCGTCGGCGGTGGTCCGCAGCGAGGCCTCTGCCAACCTGG GCGGCGTGCCCAGCAAGAGATTAAAGATGCAGTACGCCACCGGGCCGCTGCTCAAGTTCCAGATTTGTGT ATCCTGAGGGTACCGGCGGGTGTTTGAGGAGTACATGCGGGTTATCAGCCAGCGGTATCCAGACATCCGC ATTGAAGGCGAGAATTATCTCCCTCAACCAATTTATAGACACATAGCATCTTTCCTGTCAGTCTTCAAAC TAGTATTAATAGGCTTAATAATTGTTGGCAAAGATCCTTTTGCTTTTTTCGGCATGCAAGCTCCTAGCAT CTGGCAGTGGGGCCAAGAAAATAAGGTTTATGCATGTATGATGGTTTTCTTCCTGAGCAACATGATTGAG AACCAGTGTATGTCAACAGGTGCATTTGAGATAACTTTAAATGATGTGCCAGTGTGGTCTAAGCTGGAAT CTGGACATCTTCCATCCATGCAACAACTTGTTCAAATTCTTGACAATGAAATGAAACTCAATGTGCATAT GGATTCAATCCCACATCATCGATCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201303 representing NM_001040396
Red=Cloning site Green=Tags(s) MRLLLLLLVAASAVVRSEASANLGGVPSKRLKMQYATGPLLKFQICVS*GYRRVFEEYMRVISQRYPDIR IEGENYLPQPIYRHIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENKVYACMMVFFLSNMIE NQCMSTGAFEITLNDVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001040396 |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins. |
| OTI Annotation | This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001040396.3 |
| RefSeq Size | 3575 bp |
| RefSeq ORF | 588 bp |
| Locus ID | 69227 |
| UniProt ID | P62342 |
| Gene Summary | This gene encodes a selenoprotein, containing a selenocysteine (Sec) residue at the active site. Sec is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is localized in the endoplasmic reticulum. It belongs to the SelWTH family that possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif found in several redox active proteins. Studies in mice indicate a crucial role for this gene in the protection of dopaminergic neurons against oxidative stress in Parkinson's disease, and in the control of glucose homeostasis in pancreatic beta-cells. A pseudogene of this locus has been identified on chromosome 8. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210875 | Selt (untagged) - Mouse RIKEN cDNA 2810407C02 gene (2810407C02Rik), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 3,990.00 |
|
| MR201303 | Sephs2 (Myc-DDK-tagged) - Mouse selenophosphate synthetase 2 (Sephs2), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 998.00 |
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