Tnnc2 (NM_009394) Mouse Tagged ORF Clone
CAT#: MG201248
- TrueORF®
Tnnc2 (tGFP-tagged) - Mouse troponin C2, fast (Tnnc2)
ORF Plasmid: DDK
"NM_009394" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Tncs |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201248 representing NM_009394
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGACCAACAGGCTGAGGCCAGGTCCTACCTCAGCGAGGAGATGATCGCTGAGTTCAAGGCTGCCT TTGACATGTTCGATGCTGATGGCGGTGGGGACATCAGCGTTAAAGAGTTGGGCACCGTGATGAGGATGCT AGGGCAGACACCCACCAAAGAGGAATTGGATGCCATCATCGAGGAGGTGGACGAGGATGGCAGCGGTACT ATCGACTTTGAAGAGTTCTTGGTCATGATGGTGCGCCAGATGAAAGAGGATGCGAAGGGGAAGAGCGAAG AGGAACTGGCTGAGTGCTTCCGCATCTTTGACAGGAACGCAGACGGCTACATTGATGCTGAGGAGCTAGC TGAGATTTTCCGGGCTTCTGGGGAGCATGTGACAGAAGAGGAGATCGAATCCCTGATGAAGGATGGTGAT AAAAACAACGACGGCCGCATTGACTTTGATGAGTTTCTGAAGATGATGGAGGGCGTTCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201248 representing NM_009394
Red=Cloning site Green=Tags(s) MTDQQAEARSYLSEEMIAEFKAAFDMFDADGGGDISVKELGTVMRMLGQTPTKEELDAIIEEVDEDGSGT IDFEEFLVMMVRQMKEDAKGKSEEELAECFRIFDRNADGYIDAEELAEIFRASGEHVTEEEIESLMKDGD KNNDGRIDFDEFLKMMEGVQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009394 |
ORF Size | 480 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009394.2, NP_033420.1 |
RefSeq Size | 705 bp |
RefSeq ORF | 483 bp |
Locus ID | 21925 |
UniProt ID | P20801 |
Gene Summary | Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201847 | Tnnc2 (untagged) - Mouse troponin C2, fast (Tnnc2), (10ug) |
CNY 1,200.00 |
|
MR201248 | Tnnc2 (Myc-DDK-tagged) - Mouse troponin C2, fast (Tnnc2) |
CNY 1,200.00 |
|
MR201248L3 | Lenti ORF clone of Tnnc2 (Myc-DDK-tagged) - Mouse troponin C2, fast (Tnnc2) |
CNY 4,750.00 |
|
MR201248L4 | Lenti ORF clone of Tnnc2 (mGFP-tagged) - Mouse troponin C2, fast (Tnnc2) |
CNY 4,750.00 |