Rbm38 (BC006687) Mouse Tagged ORF Clone

CAT#: MG201195

  • TrueORF®

Rbm38 (tGFP-tagged) - Mouse RNA binding motif protein 38 (cDNA clone MGC:6122 IMAGE:3582604)

ORF Plasmid: DDK tGFP



  "BC006687" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,090.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Rnpc1; Seb4; Seb4l
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG201195 representing BC006687
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGATCGGGCAGCGGCTGATAGGGCTTGCAAAGACCCTAACCCTATCATCGATGGTCGCAAGGCCA
ATGTGAACCTGGCCTACCTGGGTGCCAAGCCTAGGAGCTTACAGACGGGCTTTGCTGTTGGTGTGCAGCA
ACTACACCCCACCTTGATCCAGCGCACCTACGGGCTGACTCCTCACTACATCTACCCACCAGCCATTGTG
CAGCCCAGCGTGGTGATCCCTGCCACCCCTGTCCCGTCACTGTCCTCGCCCTACCTTGAGTATACACCAG
CCAGCCCAGCCTACGCCCAGTACCCGCCAGCCACTTACGACCAGTACCCATATGCTGCCTCACCTGCTGC
AGCCACCAGCTTCGTGGGCTACGGCTACCCTGCTGCCGTGCCCCAAGCCTTGTCTGCTGCTGCACCTGCA
GGCACCACCTTCGTGCAGTACCAGGCACCTCAGCTACAGCCTGACAGGATGCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG201195 representing BC006687
Red=Cloning site Green=Tags(s)

MADRAAADRACKDPNPIIDGRKANVNLAYLGAKPRSLQTGFAVGVQQLHPTLIQRTYGLTPHYIYPPAIV
QPSVVIPATPVPSLSSPYLEYTPASPAYAQYPPATYDQYPYAASPAAATSFVGYGYPAAVPQALSAAAPA
GTTFVQYQAPQLQPDRMQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC006687
ORF Size 476 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC006687
RefSeq Size 2308 bp
RefSeq ORF 476 bp
Locus ID 56190
Gene Summary RNA-binding protein that specifically bind the 3' UTR of CDKN1A transcripts, leading to maintain the stability of CDKN1A transcripts, thereby acting as a mediator of the p53/TP53 family to regulate CDKN1A. CDKN1A is a cyclin-dependent kinase inhibitor transcriptionally regulated by the p53/TP53 family to induce cell cycle arrest. Has the ability to induce cell cycle arrest in G1 and maintain the stability of CDKN1A transcripts induced by p53/TP53. Also acts as a mRNA splicing factor. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2 (By similarity). Plays a role in myogenic differentiation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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