Ibrdc2 (BC025007) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC32473
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG201133 representing BC025007 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGCGGAAAACCCACCCTCTGGAGACCTGATTCCCGCCCCGCTCGTCACTTGCAAGCTCTGCCTGT GTGAACAGTCTCTGGACAAGATGACCATGCTCCAAGAGTGCCAGTGTATCTTTTGTACACCCTGCCTGAA ACAGTATATGGTGCTGTCAATCCGAGAAGGCTGTGGATCTCCTATCACTTGTCCTGACATGGTGTGCCTA AACCACGGGACCCTGCAGGAAACCGAGATTGCCTGTTTGGTGCCTCTGGATGAGTTTCAGCTGTATCAGC GGCTCAAGTTTGAACGAGAGGTTCACATGGACCCCCTCCGGACATGGTGTCCTGTTGCAGACTGCCAGAC CGTGTGCCATATTTCAGCCGGAGACCCAGGCCAGCCAGTGTTGGTGGAGTGCCCTTTGCCACCTGAAATT CTGCTCATGTTGCAAGGATGCTTGGCACGAGGAGTCTTCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG201133 representing BC025007 Red=Cloning site Green=Tags(s) MTAENPPSGDLIPAPLVTCKLCLCEQSLDKMTMLQECQCIFCTPCLKQYMVLSIREGCGSPITCPDMVCL NHGTLQETEIACLVPLDEFQLYQRLKFEREVHMDPLRTWCPVADCQTVCHISAGDPGQPVLVECPLPPEI LLMLQGCLARGVFL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC025007
ORF Size	464 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC025007, AAH25007
RefSeq Size	2953 bp
RefSeq ORF	464 bp
Locus ID	218215
Gene Summary	E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se (By similarity).[UniProtKB/Swiss-Prot Function]

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