Cdh23 (NM_001252635) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930542A03Rik; ahl; ahl1; bob; bus; mdfw; nmf112; nmf181; nmf252; sals; USH1D; v
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229769 representing NM_001252635 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTACTCCCTGGTCACTTGCTATGCTGTGCTCTGGCTTCTCATGCTGGTGCCTGGATCCTGGGGCC AGGTGAACCGACTACCTTTCTTCACCAATCACTTCTTTGACACGTACCTGCTCATCAGTGAAGACACGCC AGTGGGTTCTTCTGTGACCCAGCTGCTCGCCCGAGACATGGACAATGACCCTTTGGTGTTTGGCGTGTCT GGGGAGGAGGCCTCCCGATTCTTTGCCGTGGAGCCTGACACGGGTGTGGTGTGGCTCCGGCAGCCACTGG ACAGAGAGACTAAGTCTGAGTTCACAGTGGAGTTCTCTGTCAGTGACCACCAAGGGGTGATCACACGGAA GGTGAATATCCAAGTTGGAGATGTGAATGACAATGCACCCACATTCCACAACCAGCCCTACAGCGTGCGC ATCCCTGAGAACACACCAGTGGGGACACCCATCTTCATCGTCAACGCCACCGATCCTGACCTGGGCGCGG GGGGCAGCGTCCTCTACTCCTTCCAGCCCCCCTCTCCGTTCTTCGCCATCGACAGTGCCCGAGGCATAGT CACCGTGATTCAGGAGCTAGACTATGAGGTCACGCAGGCGTACCAGCTCACAGTCAATGCCACGGATCAA GACAAGACCAGACCTCTGTCTACATTGGCCAACCTGGCCATCATCATCACGGACATGCAAGACATGGATC CTATCTTCATCAACCTGCCCTACAGTACTAACATCTACGAGCACTCTCCTCCAGGCACAACTGTGCGGGT GATCACTGCTGTGGACCAGGATAAGGGACGGCCCCGTGGGATTGGCTACACTATTGTCTCAGGGAATACC AACAGCATCTTTGCCCTGGACTACATCAGCGGAGCTCTCACCCTGAATGGCCTGCTGGACCGGGAGAACC CCCTGTACAGTCACGGCTTCATCCTGACAGTGAAGGGCACTGAGCTGAATGATGACCGTACTCCGTCAGA TGCCACCGTCACCACGACCTTCAACATCCTGGTTATTGACATCAATGACAATGCCCCAGAGTTCAACAGC TCTGAGTACAGCGTGGCCATTACAGAGCTGGCACAGGTCGGCTTCGCTCTCCCTCTCTTCATCCAGGTGG TGGACAAGGATGAGGGTCTGAACAGCATGTTCGAGGTGTACCTGGTGGGCAACAACTCCCACCACTTCAT CATCTCCCCAACCTCCGTCCAAGGGAAAGCGGACATCCGCATCCGAGTGGCAATCCCGCTGGACTATGAG ACTGTGGACAGATACGACTTTGACCTCTTTGCCAACGAGAGTGTACCCGACCACGTGGGCTATGCCAAGG TGAAGATTACGCTCATCAATGAAAATGACAACCGGCCCATCTTCAGCCAGCCCCTCTACAATGTCAGCCT GTATGAGAACATCACGGTGGGGACCTCCGTGCTGACAGTCCTGGCAACAGACAATGACGTGGGCACTTTT GGGGAAGTCAACTACTTCTTCAGTGATGACCCTGACAGGTTCTCACTGGACAAGGACACGGGTCTCATCA TGCTGATAGCCCGGCTAGACTATGAACTTATCCAGCGTTTCACCTTGACTGTCATTGCCCGGGATGGCGG TGGTGAGGAGACCACAGGCAGGGTCCGGATCAATGTGCTGGATGTCAATGACAATGTGCCCACCTTCCAG AAGGATGCCTATGTGGGTGCTCTGAGGGAGAATGAGCCATCTGTCACGCAGCTGGTGCGGCTCAGGGCAA CAGATGAAGACTCCCCTCCCAACAACCTGATCACCTACAGCATCGTCAATGCATCCGCCTTCGGCAGCTA CTTCGACATCAGCATATATGAAGGCTATGGAGTGATCAGTGTGAGCCGCCCTCTGGATTACGAGCAGATA CCCAATGGGCTGATCTACCTAACAGTCATGGCCAAGGATGCTGGCAACCCTCCGCTCTACAGCACCGTCC CTGTCACCATTGAGGTGTTTGATGAGAACGACAACCCTCCCACCTTCAGCAAGCCGGCCTACTTCGTGTC TGTGCTGGAGAACATCATGGCAGGAGCCACAGTGTTGTTTCTAAACGCCACGGACCTGGACCGCTCTCGG GAGTATGGGCAGGAGTCTATCATCTACTCGCTGGAGGGCTCCTCGCAGTTTCGCATCAATGCTCGCTCTG GAGAAATCACCACCACGTCTCTGCTGGACCGAGAGACCAAGTCTGAATACATACTCATCGTGCGTGCTGT GGATGGCGGTGTGGGCCACAACCAGAAAACTGGCATTGCCACAGTGAATGTCACCCTCCTAGACATCAAT GACAACCACCCTACCTGGAAGGATGCTCCTTACTACATCAACCTGGTAGAGATGACACCTCCAGACTCTG ATGTGACCACGGTGGTAGCTGTGGACCCAGATCTGGGGGAGAATGGCACCCTGGTGTACAGCATCCATCC CCCCAACAAGTTCTATAGTCTGAACAGCACCACAGGCAAGATCCGTACCACCCACGTCATGCTGGACCGC GAGAACCCTGACCCTGTGGAAGCGGAGCTCATGCGAAAGATCATTGTCTCTGTCACAGACTGCGGCAGAC CCCCTCTGAAGGCCACCAGCAGTGCCACGGTCTTTGTGAACCTCTTGGATCTCAATGACAATGACCCCAC CTTCCGGAACCTGCCTTTTGTGGCTGAGATTCTTGAGGGCACCCCTGCAGGGGTCTCTGTCTACCAGGTG GTGGCCATCGATCTGGACGAGGGCCTGAATGGACTGGTGTCCTACCGCATGCAGGTGGGCATGCCCCGCA TGGACTTTGTCATCAACAGCACCAGCGGTGTGGTCACGACGACAGCCGAACTGGACCGGGAGCGCATCGC CGAGTACCAGCTGCGGGTGGTGGCCAGCGATGCTGGCACACCTACCAAGAGCTCCACCAGCACCCTCACC GTCCGAGTGTTGGATGTGAACGATGAGACTCCCACCTTCTTCCCTGCCGTGTACAACGTGTCCGTGTCTG AGGACGTTCCCCGTGAGTTCCGCGTGGTCTGGCTGAACTGCACAGACAATGACGTGGGCCTCAATGCGGA GCTCAGCTACTTCATCACAGCGGGAAACGTGGATGGGAAGTTTAGTGTGGGCTACCGTGATGCCGTGGTG AGAACCGTAGTCGGCCTGGACCGTGAGACCACGGCTGCGTACACACTGGTGCTGGAGGCCATAGACAATG GCCCTGTAGGCAAGAGGCGCACGGGTACAGCCACCGTGTTTGTCACAGTTCTGGATGTGAATGACAACCG GCCCATCTTTCTGCAAAGCAGCTATGAGGCCAGTGTCCCCGAAGACATCCCAGAAGGCCACAGCATTGTG CAGCTGAAAGCCACGGATGCAGACGAGGGCGAGTTTGGGCGCGTCTGGTACCGCATCCTCCACGGTAACC ATGGCAACAACTTCCGGATCCACGTAGGCAGCGGGCTCCTGATGCGAGGGCCAAGGCCCCTGGACAGGGA ACGGAACTCGTCCCACGTACTGATGGTAGAAGCCTACAACCATGACCTGGGGCCCATGCGGAGCTCTGTC AGGGTGATCGTGTATGTGGAAGATGTCAACGATGAGGCCCCTGTATTCACACAGCAGCAGTACAACCGCC TGGGCCTTCGAGAGACAGCAGGCATTGGCACCTCAGTCATCGTTGTCCGAGCCACTGACAAAGACACTGG GGACGGTGGCCTGGTGAACTACCGCATCCTGTCGGGCGCAGAGGGCAAGTTTGAGATTGACGAGAGCACG GGGCTCATCGTGACGGTTGACTACCTGGACTACGAGACCAAGACCAGCTACCTGATGAATGTGTCTGCCA CAGACGGCGCACCCCCCTTCAACCAGGGCTTCTGCAGCGTCTACGTGACACTTCTCAATGAGCTGGACGA GGCTGTTCAGTTCTCCAATGCTTCCTACGAGGCAGTCATCATGGAGAATCTGGCTCTGGGGACAGAGATC GTGCGGGTGCAAGCCTATTCTATCGATAACCTCAACCAGATTACCTACCGCTTCGACGCCTATACCAGTG CCCAGGCCAAAGCCCTCTTCAAGATAGATGCTATCACGGGTGTGATCACAGTCAAAGGCTTGGTCGACAG AGAGAAGGGCGACTTCTACACATTGACAGTCGTGGCAGACGATGGCGGCCCCAAGGTGGACTCTACTGTG AAGGTCTACATCACCGTTCTGGATGAGAATGATAACAGCCCACGCTTCGACTTCACCTCGGACTCAGCCA TCAGTGTGCCTGAGGACTGTCCTGTGGGCCAGCGAGTAGCCACAGTCAAGGCCCGGGACCCTGATGCTGG CAGCAATGGACAGGTGGTCTTCTCCCTGGCCTCTGGTAACATTGCTGGGGCCTTTGAGATTATCACCAGC AATGACTCCATTGGTGAAGTGTTTGTGGCTAAGCCCCTGGACAGGGAAGAACTGGACCACTACATCCTCA AGGTCGTGGCTTCTGACCGTGGCACTCCTCCACGGAAGAAAGACCACATCTTACAGGTGACCATTCTGGA TGTCAATGACAACCCTCCAGTTATTGAAAGCCCCTTTGGATACAACGTCAGTGTGAATGAGAATGTGGGT GGAGGCACCTCAGTGGTCCAAGTGAGAGCCACCGACCGTGACATCGGGATCAACAGTGTCCTGTCCTATT ACATCACCGAGGGCAACGAGGACATGACTTTCCGTATGGACCGTATCAGCGGTGAGATTGCTACACGGCC TGCTCCACCCGACCGTGAGCGCCAGAACTTCTACCATCTGGTGGTCACCGTGGAAGATGAGGGCACCCCC ACACTGTCGGCCACCACCCACGTGTACGTGACTATCGTGGACGAGAATGACAACGCTCCTGTGTTCCAGC AGCCACATTATGAGGTGGTGCTGGATGAGGGCCCAGACACAATCAACACCAGCCTCATCACCGTCCAGGC ACTGGACCTGGATGAGGGGCCGAATGGCACTGTCACCTATGCCATCGTGGCAGGCAACATCATCAATACC TTCCGAATCAACAAGCACACGGGTGTCATTACTGCTGCCAAGGAACTAGACTATGAGATCAGTCATGGCC GCTACACCCTGATTGTCACTGCCACGGACCAGTGTCCCATCTTGTCCCACCGCCTCACCTCTACTACCAC CGTGCTCGTGAATGTGAATGACATCAATGACAATGTGCCCACTTTCCCTCGGGACTACGAAGGACCATTC GACGTCACTGAAGGCCAGCCAGGGCCCAGAGTGTGGACATTCCTGGCCCATGACCGGGACTCTGGCCCCA ATGGGCAGGTGGAGTACAGTGTTGTGGATGGAGACCCACTAGGGGAGTTTGTGATCTCTCCTGTGGAGGG TGTGCTGAGGGTCCGGAAGGATGTCGAACTGGATAGGGAGACCATCGCCTTCTACAACCTGACCATCTGT GCCCGAGACAGGGGAGTGCCGCCACTCAGCTCCACGATGCTGGTGGGAATCCGAGTGCTGGACATCAATG ACAATGACCCAGTGCTACTGAATCTTCCCATGAACGTCACCATCAGTGAGAACAGCCCTGTCTCCAGCTT TGTCGCCCACGTCCTGGCCAGCGATGCTGACAGTGGCTGCAATGCCCTCCTCACCTTCAACATCACCGCT GGCAACCGAGAGCGGGCCTTCTTCATCAATGCCACGACAGGGATTGTCACTGTCAACCGGCCCCTGGACC GAGAGCGCATCCCAGAGTACAGGCTGACTGTTTCTGTGAAGGATAACCCGGAGAACCCACGCATAGCCAG GAAGGACTTTGACTTACTGCTGGTATCTCTGGCTGATGAAAACGACAACCACCCCCTCTTCACTGAGGGC ACGTACCAGGCCGAGGTGATGGAGAACTCTCCTGCTGGGACACCCCTCACAGTGCTCAATGGGCCTATCC TGGCCCTGGACGCTGATGAAGATGTGTACGCCGTAGTCACGTATCAGCTCCTGGGCACCCACAGTGACCT CTTTGTTATCGACAACAGCACAGGCGTGGTGACTGTGAGGTCCGGGATCATCATTGACCGGGAGGCCTTC TCACCCCCGTTCCTGGAGCTGCTGCTGTTGGCTGAAGACATTGGACAACTCAATGGCACGGCCCACCTGT TCATCACTATCCTAGATGACAATGACAACTGGCCCACCTTCAGTCCTCCCACTTATACCGTCCACCTGCT GGAGAACTGCCCACCAGGATTCTCAGTCCTTCAAGTCACAGCCACGGATGAGGACAGCGGCCTCAATGGG GAGCTGGTATACCGAATAGAAGCCGGGGCTCAGGACCGCTTCCTCATCCACCCAGTCACAGGAGTCATCC GTGTTGGCAATGCCACCATCGACAGAGAAGAGCAGGAGTCCTACAGGCTGACGGTGGTGGCCACCGACCG GGGTACCGTTCCCTTGTCGGGCACAGCCATCGTCACCATCCTGATCGACGACATCAATGACTCCCGGCCC GAGTTCCTCAACCCTATCCAGACAGTGAGCGTGCTGGAATCCGCAGAGCCAGGCACCATCATTGCCAATG TCACCGCCATCGATCTCGACCTCAACCCCAAACTGGAGTATCACATCATCAGCATCGTGGCCAAGGATGA CACCGACCGCCTGGTGCCTGACCAGGAAGACGCCTTTGCTGTGAATATCAATACAGGGTCTGTGATGGTG AAGTCCCCTCTGAACCGCGAGCTGGTTGCAACCTATGAGGTCACGCTCTCAGTAATCGACAATGCCAGCG ACCTCCCAGAGCATTCTGTCAGTGTGCCAAACGCCAAGCTCACGGTCAACATCCTGGATGTCAATGACAA CACACCGCAGTTCAAGCCCTTTGGGATCACCTACTACACAGAGCGGGTCCTGGAGGGGGCCACCCCCGGC ACGACGCTCATTGCCGTGGCAGCCGTGGACCCTGACAAGGGCCTCAATGGGCTGATCACCTACACCCTGC TGGACCTCACACCCCCAGGCTATGTCCAGCTCGAAGACTCTTCCGCAGGGAAGGTCATCGCCAACCGGAC TGTAGACTACGAGGAAGTGCACTGGCTTAACTTCACTGTGAGGGCCTCAGACAACGGGTCCCCACCCCGG GCAGCCGAGATCCCTGTCTACCTGGAAATTGTAGACATTAATGACAACAACCCCATCTTCGATCAGCCCT CCTACCAGGAGGCCGTTTTTGAGGATATAGCTGTGGGCACGGTCATCCTGAGGGTTACAGCTACGGATGC GGACTCGGGCAACTTCGCCCTCATTGAGTACAGCCTGGTAGATGGAGAGGGCAAGTTCGCTATCAACCCC AACACCGGCGACATCTCTGTCCTGTCCTCTCTGGACCGAGAGAAGAAGGACCACTATATCTTAACTGCCT TGGCCAAAGACAACCCTGGGGATGTGGCCAGTAACCGTCGAGAGAACTCGGTACAGGTGGTGATCCGAGT GCTTGATGTCAATGACTGTCGGCCTCAGTTCTCCAAACCGCAGTTCAGCACGAGTGTGTACGAGAACGAA CCAGCAGGCACCTCGGTCATCACCATGCTGGCCACCGACCAGGATGAGGGCTCCAATAGCCAGCTGACCT ACTCTCTCGAGGGCCCTGGAATGGAGGCTTTCTCTGTGGACATGGACTCAGGCCTGGTGACCACGCAGCG GCCGCTGCAATCCTATGAGAGGTTCAACCTGACTGTGGTGGCTACAGATGGTGGGGAGCCCCCACTCTGG GGCACCACGATGCTCCTGGTGGAGGTCATCGATGTCAATGACAACCGACCCGTCTTTGTGCGCCCACCCA ATGGCACCATCCTGCACATCAAAGAGGAGATCCCGCTACGTTCCAATGTGTATGAAGTCTATGCTACAGA CAATGACGAAGGCCTCAATGGGGCAGTACGCTACAGCTTCCTGAAGACTACAGGCAACCGTGACTGGGAG TACTTCACCATTGACCCAATTAGCGGCCTCATCCAGACAGCGCAGCGCCTGGACCGTGAGAAGCAGGCCG TGTACAGTCTCATCTTGGTGGCCAGTGACCTGGGCCAGCCGGTGCCATATGAGACGATGCAACCATTGCA GGTGGCCTTGGAGGACATCGATGACAACGAACCCCTCTTTGTGAGGCCTCCTAAGGGAAGCCCTCAGTAC CAGCTGTTGACGGTGCCTGAACACTCCCCCCGCGGCACCCTTGTGGGCAATGTGACAGGTGCCGTGGATG CAGATGAGGGTCCCAACGCCATTGTATACTACTTCATTGCAGCTGGTGACGAGGACAAGAATTTCCACCT GCAGCCCGATGGACGCCTGCTAGTGCTCAGAGACCTGGATCGGGAGACGGAAGCCACCTTCTCCTTCATC GTCAAAGCGTCCAGCAATCGCAGCTGGACCCCTCCTCGTGGACCCTCCCCAGCCCTCGATCTGCTGACCG ATCTCACCCTGCAGGAGGTGCGCGTTGTGCTGGAGGACATCAATGACCAGCCGCCACGCTTCACCAAGGC TGAGTACACCGCAGGAGTGGCCACCGATGCCAAGGTAGGCTCGGAGTTGATCCAGGTGCTGGCCCTGGAT GCGGACATTGGCAACAACAGCCTGGTCTTCTATGGCATTCTGGCTATCCATTACTTCCGGGCCCTGGCCA ACGACTCTGAGGATGTGGGCCAGGTCTTCACCATGGGGAGTGTGGACGGTATCCTGCGCACCTTTGACCT CTTCATGGCCTACAGCCCTGGCTATTTTGTGGTAGACATCGTGGCCCGAGACCTGGCCGGCCACAATGAT ACCGCCATCATCGGCATCTACATCCTGAGGGATGACCAGCGCGTGAAGATCGTCATCAATGAGATCCCGG ACCGCGTGCGTGGCTTCGAGGAGGAGTTCATTCGCCTGCTGTCCAACATCACAGGCGCCATTGTCAACAC CGATGACGTGCAGTTCCACGTGGATATGAAAGGGAGGGTGAACTTTGCGCAGACAGAACTGCTTATCCAC GTGGTCAATCGCGACACAAACCGCATCCTGGATGTGGACAGGGTCATCCAGATGATCGATGAGAACAAGG AGCAGCTTCGGAACCTCTTCAGGAACTACAACGTGCTGGATGTGCAGCCCGCCATCTCTGTCCAGCTGCC GGATGACATGTCTGCCCTGCAGATGGCCATCATTGTCCTGGCCATCCTTCTCTTCTTGGCTGCCATGCTC TTCGTCCTCATGAACTGGTACTACAGGACCATACACAAGAGAAAGCTAAAGGCCATTGTGGCTGGCTCTG CGGGGAACCGTGGCTTCATTGACATCATGGACATGCCCAATACCAACAAATACTCCTTCGATGGAGCCAA CCCTGTGTGGCTGGACCCCTTCTGCCGGAACCTGGAGCTGGCTGCGCAGGCCGAGCACGAAGATGACCTC CCTGAGAACCTGAGTGAGATCGCAGACCTGTGGAACAGCCCCACCCGCACCCATGGAACTTTTGGGCGTG AGCCTGCAGCAGTCAAGCCCGATGATGACAGATACCTGCGTGCAGCCATCCAGGAATACGACAACATCGC CAAGCTGGGCCAGATCATTCGAGAGGGGCCTATTAAGGGCTCGCTGCTGAAGGTGGTCCTGGAGGATTAC CTACGGCTCAAAAAACTCTTTGCACAACGGATGGTACAAAAAGCCTCCTCCTGCCATTCCTCCATCTCCG AGCTGATCCACACCGACCTGGAGGAGGAGCCTGGGGACCACAGCCCAGGCCAGGGCAGCCTCCGTTTCCG ACACAAGCCACCCATGGAGCTCAAGGGGCAAGATGGAATCCACATGGTTCATGGCAGCACGGGCACACTG CTGGCCACTGATCTCAACAGCTTGCCGGAGGACGACCAGAAGGGGCTGGACCGCTCGCTGGAGACTCTGA CGGCCTCTGAGGCCACTGCCTTTGAGCGCAATGCCCGCACAGAGTCGGCCAAGTCCACCCCTCTGCATAA GCTCCGGGACGTTATCATGGAGAGCCCTCTGGAAATCACGGAGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252635
Insert Size	10059 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252635.1, NP_001239564.1
RefSeq Size	11090 bp
RefSeq ORF	10059 bp
Locus ID	22295
Gene Summary	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. CDH23 is required for establishing and/or maintaining the proper organization of the stereocilia bundle of hair cells in the cochlea and the vestibule during late embryonic/early postnatal development. It is part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.