Igfn1 (NM_177642) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9830123M21Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229762 representing NM_177642 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGAGATGCGAACACAAGTTAGAATTGAGTTTCATGCTTTGGCAACTCAAAGGAAGCCTTCAGCAT CACCTCCTACCATTGAGGAGGACATGGGCCAAGGTTGTACTCTAGAAGCTCTGGTCATCCCTAGCAGAAG AGCTGAGCAATCAACTCAGCACCCAGACAGCTCATTCATGGCCACGGTCAGAGGTGCTAAATCTGTAGTA GTCAGTCAATGGCAGCTGCTACAGGCCGGATCCGCAGGCTCTGCCGCCAGACCAGACGGCTCCGGCTCTG AGTCCCTGGCAGCCAGCAGCAGCTGGAAACCAAGAAGGAGGCTGGAAGCTGGTGGGCCATGCAGATCATG GCTGGTGTTTCTGGTGTCACGGGAAGGATGGAGTGATAGCAACCCGGGCTATCGTAAACAATTCATCTGT CGCCCTCAGGAGATCGGTTTCCACTCAGAGGAGCACAGAGAAGCTGGGACTATGGCTGCAAAGCCCCCTA AGAAGTCCTCCATCCCAGGAGTGAGCATCAGGCAGCTGGTGGACGAGATCCCAGAAGGCTGCAGCACACC AGATTTCAAGCAGAAGCCTGTTACCTTGGCTCTACCAGAGGGGAAAAACGCCATCTTTCGGGCGGTGGTC TGTGGGGAACCCAGACCTGAAGTGCACTGGCAGAGCAGCAAAGGAGACCTCAGCAACTCCGCCAAGTACC AGATATCCTCTGCTCCCGGTAGAGAGGAGCACGTGCTTCAGATCAACAAGCTGACTGGAGAGGATTCGGA TCTGTACCGCTGCACTGCACTAAATGCCTATGGAGAAGCCACATGCTCAGTGAGGCTTACGGTCATTGAA GTGGGTTTTCGGAAGAACCGAAAAAGGCAAAAGGAACCTCAGGAGGACCTCAGGAAGGAGCTGATGGACT TTCGGAAGATGCTGAAAAAACGGGCCCCACCCCCAACCCCTGAAAAAAAGATGGAGTCTGAGCAGGTGTG GCAGCTTTTGGTGACTGCAGACCGTAAGGACTACGAGAAGATCTGTATGCAGTATGGTATCGTCGACTTC CGGGGCATGCTGCGCAAGTTGCAGGAGATGAAAAAGGAACAGGAGGACAGAATGGCCCAGTATGTCAGTG CCATTGCCAACTTGAGACACATCAAGGTCACCAAGGAAGGGGTCGCTACGTTTGACCTGGAGCTGGATCT CAAGAACCTCGAGAGCAAGATTTACTTGTACAAGGATGGTGAGATGATTCCCTACGGTGTTGATAACCAG ACCAAGCACTGTCTGAGACGGCTGGGGAAGCGCTACCACTTCCAGATCCAGGACCTGCGGCCTGAGGATG CTGGCCTTTACCAGGTCATGGTGGAGGATGCTGTGGTCTTCTCTACAGAGCTAGAATCCAGAACCATCCC CCCCAGAGTGGTGGTCCCATTGGCAGAGACCCGCTGTGAGGAACGGGGTGATGCAGTCTTTGAATGTACC CTCTCCAATCCCTGTCCCAGTGCGACCTGGCATTTCCAGCACCGACCACTGCGGCTCAGTGACAAATATG AAGTATTTGTGTCCCCTGATGGGCTAACCCACCGACTGGTTGTGAAGGGGGCCAGTAGCTCAGACATGGG CCTCTACTCGCTGAACACTGGGCTCCATGCCTCCAGTGCCTGGCTGGTGGTTGAAGGTGGGAAGGATAAA GGCCCTCAGACCACAGATACTGACCACCGGCTGCAGACTCCAGAAGCTCTGGCCTCCGAAGCAGAAGACG CTGGAGGCATCAGCATCAAGGGAGGGCAATCCAGAGAGCGGGGTTCCCTCAAAGAGATTAGTGGGGCACA GCTCACAGCAGGCCCAGACAGGGGTGCCTTTGGCAAACATGGCTATCCCTTGGTGGTGGATGAAGACATA GTTAATTCTACTTGGGGACCTGGGCAGGACAAAAAGAGCTTTCTGGAAGGAGGACAACTCAGGGTCACTC TTCCAGGGGATAGTCAGTCCCAAAGAGAGGGAGACTGTGGTAGAAGCCTCCCCAGAAGGTCTCATCTCCA GGGAGAGGATACAGAATCAGATTTGGGCTTCCTGGAGAGAGGACAACAAGGGCCTGGTAGAGTTGACAGT GAGAATGGCAGATGGGAGACAGCAGGAGGCCAGAGTGCTGGCTCCAGCCATCCTCGGGACAGAAGGCTTG AGAGCAGAGGAGAAGGACAGGAACACAGTGAGGGCCATGGCAGTGAACTGGACAGATATGGCCAGGGACA ATGCCATGATCCTCAGCTGGGAGCAGGGGCAGGGCAGAGGGTGCCGTGGGGATCCCAGTTTGATGCTGAG GGGCTTCAGCTGAAAAAGGAGGGGACAGAAATGTGGGGCGATTGTCTGGATGTGACGGAAGGTAGGGGTA ACCTGAGAAAGGAGAGGGGAACAGCAGCAGGGTCAAGTAACAGAGCCCGCCTCTGGGAAGCTCGAGGAGG CAGTGGGGCCGGAAGTCCTTGCGTCCCTGAGTTTCCTAGAGGAGGAGCTCACAGCTCCAAGGCAGGCATG GGCCCTGAGTGCTGGCCAGGTGGAGATGCTGGCCATGGAGAAGCTGGGGTATACTGGGGGGCAGGGGGAT ATCCAGGACAGACATCTGAGGGCAATGACACCCAGAAATCATCACTATCTGGAGACAGGAAACTTCTGGG GTGCACGAGTCCTGAGGCCAAAGCTGAGGGCTCTTTCCAGAGCGCAGATGGTCATGGGATTGTGGGGAGG GTCTACAAACCTGGCCCTGAAGGCCCAGAAGATCTTAGGAGTCAGAAAAGTGGCCTACAAGAGATTCAGG GAAGGAATGGTCAAGGCTCAGCTGGAGCCTTGGGCAGGACGGTGGAAGGTTCCAGAAGCCTCCAATTTCC TCAGAGCTGGACAGTGGGACAGAGGGAAGCAGGGAAGCCAGGTGGAGCAGAGTATGAAGACATAGGTCCT CAGGATGACACGTGGTCCAATCTCAGAGAGATGGGAGGCCACTGTGGGTCTGGAGTGCTGGCGTCTGGTG GGAGGGAAGGGAGCGTGGGCGGTTCCCAAGTAGCTGCCCTGATGATGTCTAGTCAGAGGGTAGATGCCAG AAACCACAGGCTAGTTACATCCCCAGGCCTGGGTGTCCAGGGTTCTGGCAGAACACTGGGACACATGGAA GGATTAAGAGACCCAGAGGCAGTGGGATCCGAAGAAGAGTTCTGGAATGGGTCAGGAAGGTCCCAAGGAA AAGGGTCCAGAGGTGGGATGGGCTTAGGAGGTCCAGAGTGGGCAGAGTCTAGGAATGAGGAGGGATGGGA GAATTCTGAAGGGATGGCTTCTAGGAATGGAGCCACTTGTCGTGATGGATCCAGGATGCAGGAAGGAACA GGATCTGGCCCTGATATTGGTTGCAAGGCCCCCTGTGGGGCACCTGCGGAGACAGAGTCTGGGAAGTGGG GTACTTACAGTCATGACTCAGGGGTGCCTGGGGGATTGTGGTCTGGAAGCAAAAGTCAGTGTAGTCCTGC AGGAAAGGTATCTGGAAGAGAGGCCAGCCTCAGAAATGGCTCAGCTGGCCTTCATGGAATGCCTTCTAGT GAAGCTCAGCAGAGGGATGTATTTCAAGGCCACGGTCAGACAGGCCACATTGGAGAGCACCACTGTGCCC GTGGCTTAGGGAGTTCTGAGACAGTGGGATCTGTGGGTGGACATGGTAGGAAAGACTCTGGAACAGCGGG CAAGGTTGGAGAGGGATACACAGAAGCAGAACCAGGGCACTCTGGAGGGTTGAGTTCTTGGGGTCACACT GGAGATTATGAGGACTTCCGAGTCCTGGGGGCCTTTAGGGAAGGAGACTTTGGCAATGGCACTGGGGTTC CATTATCCATGGGACCAAGGTCTCTGGAGAGAGGGGACAAGGAAGGAGATGGAGAAAGGATCAGTTTTCT TGGTGCCAGGACCTCTACAGTTGGAACTGGGAATTGGGACAAAGCCAGGCACCCTGGGGCACCATCTCCT CATGAGGCCGGTTCAGAGGGCCACTGGGCTCAAGTGTCTGGCAATGCATTAGGTTGTAGAGATGGGTCTG GAATTCCAGAGCCTTGGAGTGCTGGCGACAAAACAGCTTATGGAGAAGAATCAAAGGGTCTTGGGCCTGA AAGAACAGGACCAGATGGTGAGGTGGCATTTAGAGGTAGCTCTTCGAGCCTCAGAGGAATGGGACCAGCA AGTGAGGCAAGTTATAAAAGTGGAACTTGGGGCCCTGGGGCTGTGGGTTCAGGGTTTAAGGTAGATTGTA GGAATGATCTTGGGTGTTCTGGAAGTATGGGGTCAGAGAGTAAAGCAGGTTATAAGAACAAAATTGAGAG TCATGGGGTCAGGGAGTTAGGGGACCTGACAGGCTATAAAAATGATTTAGGACCTTCTGAACTTATTTCA TCAAGGAATGAGGCACGTGGTTTAGAACATTCTGGAGACATTTTGTCATGGAATAAGGCAGGTTTAAGGG ATGGCTTGGGAGAAACAGGAAGAATGGAGTCCAAGAATGGGGTTGGTTATAGGGGAAGCTCAGTGGGTCC TGGGGAAATGGGGTCAGAGAGGAAAATGCACCTTGCAGATGGTTCAGGGAGGCTAGGAGGACCAGGGTCA CTGGCTGCGCCCAAAGAACATGAATCTGTGGGTCTTGGGTCAGTTTATGAGGGAGCAGCAGAGATTGGCT CCAAATATAATAGGGAGAAAGTCAGAGGAATGGGGTTTAGAGATGCAACAGGGCCTGAGGTGGGCTCTGG GGTAGCTGGAATGCTGGATATTACAGGTGGCATAGCACACGGGGACAGTGCTATGGGCACAGAGAAGCTG GGGACACCAGATAGGCCTTATATCTTGTCAGGTGGGCAGGGTACCAAGAACAGTCTTGGGGGCTGTGTGT CCTTGGGAATCCCTGACGCCTTGGGGGCTGTTGGCTCTGTGGGGAAACCAGGTATCAGAGAATGGAAAGA TGATTCTGGGTTCCAAGGGTCTCTCAGGGACAGAGGGACTCCGAGTGAGGAGATCAGATCTGTAGACCAA GCAGGAGCTATAGGGACATCTCGGCTTCTTGATAGTAGGGGGGCAGTGGAGGATGGGAGTGGGTCAGAAA ATGACTTTGACTTTAATAAATCAGGCTTGGAGACAACAGGAAGATGTAGAGTTGCTGACCAGCAGGGAGT GACACCACAGGGATGTGGAGGCTCATTGCTCAATGGCAGGAGGAAGGGAACAAGTTCTGGAAGCTTGTCA GGGGTAGGGCAGGAGGTAGACAGAAGTTCTACACCTGGGAGGGAGACCAAGGTAATGTCAGGACCTGGCA GTGGGCGAGCCACAAGCCACACTCGGGCTGCAGACTGGGAAGACGGGGTTCAATGCAGCTTCGGGGCTTC TAGCTCACTGCCTGATACTGATGCCATTTTCAGAGAAACCCATGCGGGACAAGGAGCCTTCAAGGGCAGG GGCTGTGAAACCGGCCAAGGGGCTGCTGGAGAGTGTCAAGGACCACGCTCCTTGGGGAGCAGAGGCTCAG AGTTTGTAGGGGGCAGGGCTGGATTTACAGGGAGCTCAAGTGTTCCAGGCAGAAGGGATTCCACTATCTA CGGAGATGCTGTCACATCAAAGCCACAGGAACCCCAGAATGAATTATATAGTCCTTTAGGCAGAAGGGAC TTCAAAAGTGTATCGGGAGGAATCCAGGAGCCAGGTTTTCAACGAGGAACAGGACAGGGAGAAGGAAAAG AATCCTTCCAGGAGTCAGGGTCCTGGGAGGCTGAGCTTGACGAGGTCCAAGGATCCAGGGCCTCAGCAAA GTTTGAAAACCAGGGAGGGAAAGACCCTGGCTGGTCAGGCCAGAAGCCTGGTCCCTGTGGAAGCAGGCAT CAGGTGCAGTCTGGGACTGAAGTTGGATCAGCAAAGAGAGCAACCCCACAGGGGGCCGGAGGGCTGGAGC CTTGGGCTGGCAGTGAGGATAGAGGGTCTCTGAGAGAACCTTGGAGTGAAGACAGGAGGCAAGGCCCCCA CAGGCACCTTGGCAGCAGGAGAGATACCCAAGAGGGCAGGTCAGATGTCTGTGGCCAGGCACAAGATGCT ACCCAGAGCCCCAGATCCAGATACCAGCCTGGCACTGGCCGCTCTTCTTCAGAGGCCCGAGGCTCCATGG ACCACTTCTCTCAGGGTCTGACTGATACAGAGGTGCAACTGGGGGAAGCCGCCATAGTCTCCTGTACTCT TGCCAGTGACCTGGGACCTGGCACGTGGTTCAAGGATGGAGTCAAGCTCACTGCCCAAGATGGAGTCATC TTTGAGCAAGATGGGCTCACGCACAGACTGATTCTCACCCACGTGGAGGGGACCCAGGCTGGGAAATACA CTTTTGTAGCCGGCTGCCAGCACAGCGAGGCCAGCCTGACCGTGCAGGATCCCCCCACCATTGCTCCAGA TGTGACAGAGACACTGAGAGAGCCACTGGTGTTCAAGGCTGGGAAGCCAGTCACCGTGAAAATCCCTTTC CAGAGCCGCCTCCCTGTTCAGGCTGCCTGGAGGAAAGATGGGAACGAGGTGGTGGGCGGCGACCACAAGG GCATCCAACTGGCTCTGGCAGATGGCTACACATGGCTGTGCCTCCCCAGCGTGTGCAGGAAGGACAGTGG CCGGTACAGCGTGACGCTGAGGAGCGAGGGAGGCTGTGTGCAGGCTGAGTTCACCCTGCAAGTTATAGAC AAGCCTCAGCCCCCACAAGGACCCCTGGAGGTTCAGGATTGCCACAGAGCAGGTGTCTGCCTCCGCTGGC TACCCCCGAGGGACAACGGGGGCCAGGTCATACAACACTATGTGGTGGAGAGGCGACAGGCTGGAAGGAG CACTTGGTTGAAGGTGGGCGAGCCCCCATCAGACAGTACCAGCTTCACCGACACCAGCGTGGAGCAGGGA AAGAAATATGCCTTCCGTGTGCGGGCTGTGACCTCAGTGGGAGCCGGAGATGCCCTGGAGTCAGAGGAGG TGTTGGTGGCTCCTGAGGCTCTCCCGGGGCCCCCTTCTGCCCCAGCCATCCTGTCAGCCTCCAGTCAGAG CATCACTCTCACATGGGGGGCACCACAGGGCCCTGGCAGTAACCACATCCTGGGCTACCTGATCGAGAAG CACAAGAAGGGGAGCAACACCTGGATGGCTGTGAATGAGCAGCCCGTGTCTGAAAGAAGGTACACTGTGG TGGGTCTGCGACAGGGCTGCCAGTATGAGTTCCGGGTCACGGCCGTGACTCTGTCGGGCCCTGGAGATCC TGGGCCTCCATCGGATGCTGTCTTTGCTAGAGACCCTATGAGACCCCCAGGGCCTGTGCGGGATCTACAG GTTACAGACACATCAAACACTAGCATCACCGTGAGCTGGATGGTGCCGGATGCCCGGGATGCTGATGAAG CCCAGGGATATATTGTGGAGTTGTGTGGTTCAGACAGCCATCAATGGAGCCCATGTCACGTGGGAACTGT GCCGGGCACCACCTTCACAGCCAAGGGGCTTCGGCCGCAAGAAGGCTACTTTGTGCGGGTGACAGCAGTT AATGATGGGGGCCGGAGCCAGGCCACTTCCCTGGACACATTGGTTCATGCCATGCCGGCCACTGTCTGTC CCAGGTTCCTCATGGACTCCGGCACAAAGGATACCCTGATGGTCAGGGTTGGGGACAGTATTCGCGTTCC TCTCTCCTTTGAAGCTGCCCCCATGCCTGAGGTGACCTGGCTGAAGGACGGCTTGCCCTTGCCCAAAAGA AGTGTGACCACTGTGAAGGCTGGCCTCACGCAGCTTCTGATCCCTGCAGCTAGCCTCTCGGACTGTGGCC GATACACCGTGATGCTGAGGAACCTACAGGGGAAGGAAGCTACCCACAGCTTCTTCATCAATGTAGCAGC ATGCCCACAGGCACCAGGTTCTATCTACCTACAGGAGAATGTGCCTGGGACAGTGACGGTCCAGTGGGAA CCCTCTCCGGATGAGGCTCAGGGCATCCCCTTGCATTACACAGTGCTGATGCGGTCCTCATCTCACCGAT CCTGGCACGAGGTGGCCGACCATGTCCGTACCAACCGCTTTACCCTCCTAGGGGTCCTCCCTGGCCATGA GTACCACTTTCGGGTGCTGGCCAAGAATGAGCTGGGGGTCAGCAAACCTTCAGACACCAGCCAACCCTGG TGTATCCCCAGGCAGAGAGACAGGTTCACAGTGAAGTCTCCGACATACCAGGATCCTGACCTGAGCCAGA AGCCCCGCTTCCTGGTGGGTCTGCGGGCTCACCTTCTGCCCCAGGGCTGTGAGTGCCGCATGACGTGTGC CGTGCAGGGCTCACCCCAGCCTCATGTCACCTGGTTCAAGAATGACCAAAGCTTGGACAGAAACCCTGCG GTGTACAGTACTGACTTGCTGGGTGTGTGCTCCCTCGTCATCCCCAGCGTGTCCCTTAAGGACAGTGGTG AATACAAGGCTGTGGCCAAGAACCCACTGGGACAAGCTGTCAGCACAGCCACCCTCATTGTCACAGAATA CGACTCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177642
Insert Size	8550 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177642.3, NP_808310.2
RefSeq Size	8996 bp
RefSeq ORF	8550 bp
Locus ID	226438
UniProt ID	Q3KNY0

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