Kmt2b (NM_001290573) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610014H22Rik; mKIAA0304; Mll2; Wbp7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229760 representing NM_001290573 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGGCGGCGGCAGTTGCCCCGGGCCTGGCTCCGCACGGGGCCGCTTCCCGGGCCGGC CGCGGGGTTCCGGCGGGGGCGGGGGCCGCGGCGGCCGAGGCAACGGAGCCGAAAGAGTGCGGGTAGCCCT GCGGCGCGGTGGCGGCGCGGCGGGGCCGGGAGGAGCCGAGCCCGGGGAGGACACGGCCCTGCTCCGTTTG CTGGGTCTTCGCCGGGGCCTGCGCCGGCTCCGCCGCCTGTGGGCTGGTGCGCGAGTTCAGCGAGGCCGAG GCCGCGGCCGGGGACGGGGCTGGGGCCCGAACCGAGGCTGCATGCCGGAGGAAGAGAGCAGTGACGGGGA ATCCGAGGAGGAGGAGTTTCAGGGTTTTCATTCAGATGAAGATGTGGCCCCCAGTTCCCTGCGCTCTGCG CTTCGGTCCCAGCGAGGTCGAGCCCCTCGGGGTCGGGGCCGCAAGCATAAGACGACCCCCCTTCCTCCTC GCCTAGCAGATGTGACTCCTGTCCCCCCAAAGGCCCCTACTCGGAAACGGGGTGAGGAGGGCACAGAACG GATGGTGCAGGCACTGACTGAACTTCTCCGGCGGTCCCAAGCACCCCAACCCCCCCGGAGCCGGGCACGG GCACGTGAACCCTCTACTCCCCGACGGTCTCGGGGAAGGCCCCCAGGACGGCCAGCCGGTCCCTGCCGGA AAAAGCAGCAAGCAGTAGTGTTAGCAGAAGCGGCTGTGACAATCCCTAAACCCGAGCCTCCGCCCCCTGT GGTTCCAGTAAAGAACAAAGCTGGCAGTTGGAAATGCAAGGAGGGACCTGGTCCAGGACCTGGAACCCCC AAACGTGGAGGACAGCCTGGGAGAGGAGGCCGTGGAGGCAGGGGCAGAGGCCGAGGCGGACTTCCCCTTA TGATCAAGTTTGTTTCCAAGGCCAAGAAAGTGAAGATGGGACAATTGTCCCAGGAACTAGAATCAGGTCA GGGTCATGGTCAACGTGGGGAAAGCTGGCAGGATGCCCCCCAAAGAAAAGATGGAGATGAACCAGAAAGG GGCTCGTGTAGAAAGAAACAGGAGCAGAAACTGGAGGAGGAGGAGGAGGAGGAGGAGAAGGAGGGAGAAG AGAAGGAAGAAAAAGATGACAATGAGGATAACAACAAGCAAGAGGAAGAGGAGGAGACTGAGAGAGCTGT GGCTGAGGAAGAAGCGATGCTAGCCAAGGAGAAGGAAGAGGCAAAGCTTCCGTCACCACCCTTGACTCCT CCAGTCCCTTCACCACCTCCTCCTCTACCACCCCCTTCAACATCTCCTCCACCTCCAGCATCCCCTCTAC CACCCCCAGTTTCTCCTCCTCCCCCACTTTCCCCTCCCCCATATCCAGCCCCAGAGAAGCAGGAAGAGTC TCCTCCTCTTGTCCCAGCTACATGCTCTAGGAAGAGGGGCAGGCCTCCCCTGACTCCTAGCCAGCGGGCA GAGAGGGAAGCTGCTCGGTCAGGACCAGAGGGCACCCTTTCTCCCACTCCAAACCCTAGCACCACCACAG GAAGCCCTTTGGAAGACAGTCCTACTGTGGTCCCCAAAAGTACCACCTTTTTAAAGAATATACGGCAGTT TATTATGCCTGTAGTGAGTGCTCGCTCCTCCCGTGTTATCAAGACACCCCGGCGATTTATGGATGAAGAT CCTCCCAAACCTCCAAAGGTGGAGGCTTCTATTGTTCGACCTCCTGTTGCAACCTCCCCACCTGCTCCCC AGGAACCAGTACCAGTCTCCTCTCCACCTCGTGTCCCAACTCCCCCATCTACCCCAGTCCCACTCCCTGA GAAGAGGCGGTCTATCCTAAGGGAACCCACATTTCGCTGGACCTCACTAACCCGGGAGCTGCCGCCACCT CCACCTGCTCCTCCACCAGCCCCATCTCCACCACCTGCCCCTGCCACTCCCTCCCGGAGGCCCTTGCTCC TTCGGGCCCCTCAGTTTACCCCAAGTGAAGCCCACCTGAAGATCTATGAATCGGTGCTTACCCCTCCTCC TCTTGGGGCTCTTGAAACCCCTGAGCCAGAGCTGCCTCCTGCTGATGACTCTCCAGCAGAGCCTGAGCCT AGGGCAGTGGGGCGGACCAATCACCTCAGCTTGCCTCGCTTTGTCCCTGTGGTCACCAGTCCTGTTAAGG TTGAGGTGCCCCCCCATGGAGCTCCAGCTCTGAGTGAAGGCCAGCAGCTTCAGCTCCAGCAGCCCCCGCA GGCCTTGCAGACCCAGCTCCTACCCCAGGCACTACCACCACAGCAACCACAAGCGCAGCCACCTCCATCA CCACAACACACACCACCACTGGAAAAGGCCCGTGTTGCAAGTCTGGGTTCCCTGCCACTATCTGGGGTGG AGGAGAAAATGTTTAGCCTTCTTAAGAGAGCTAAGGTGCAGTTATTCAAGATCGATCAACAGCAACAGCA GAAAGTGGCGGCTTCAATGCCGCTAAGCCCTGCAGTACAAACCGAGGAGGCTGTGGGGACTGTCAAACAG ACCCCGGACAGAGGCTGTGTCAGGTCTGAAGATGAATCCATGGAAGCTAAGAGAGATCGAGCCTCGGGCC CTGAGTCACCTTTACAAGGCCCCCGAATCAAACATGTCTGCCGTCACGCTGCTGTGGCCTTGGGTCAGGC TCGGGCCATGGTGCCTGAAGATGTACCCCGCCTCAGTGCCCTCCCTCTCCGGGATCGGCAGGACCTCGCC ACTGAGGATACGTCATCAGCATCAGAGACTGAGAGTGTCCCTTCCCGATCCCAGCGGGAAAAGGTGGAAT CTGCAGGGCCTGGGGGAGACTCGGAGCCTACAGGGTCTACAGGGGCCCTGGCCCACACACCACGGCGGTC ACTGCCATCCCACCATGGCAAGAAGATGAGAATGGCTCGTTGTGGGCACTGTCGGGGTTGCTTGCGTGTG CAGGACTGTGGCTCTTGTGTCAACTGCTTGGATAAACCTAAATTTGGGGGTCCCAACACTAAGAAGCAGT GCTGTGTATACCGGAAGTGTGACAAGATAGAGGCTCGGAAGATGGAGCGGCTGGCAAAAAAAGGCCGGAC GATAGTGAAGACGCTGTTGCCCTGGGATTCCGATGAATCTCCTGAGGCCTCCCCTGGTCCTCCAGGCCCA CGCCGGGGGGCGGGAGCTGGGGGGTCCCGGGAGGAGGTGGGGGCCACTCCCGGGCCCGAGGAGCAGGACT CCCTCCTACTGCAGCGCAAGTCAGCCCGACGCTGCGTCAAACAGCGGCCCTCCTATGATGTCTTCGAGGA CTCAGATGACTCAGAGCCCGGGGGTCCTCCTGCTCCAAGACGTCGCACCCCCAGAGAACATGAGCTGCCA GTACTAGAACCTGAGGAGCAGAGCCGGCCCCGCAAACCCACCCTGCAGCCTGTGTTGCAGCTCAAGGCCC GAAGGCGCCTGGACAAGGATGCTTTAGCCCCTGGCCCCTTTGCCTCTTTTCCCAATGGCTGGACTGGAAA GCAGAAGTCTCCTGACGGTGTGCACCGGGTCCGAGTAGATTTTAAGGAGGATTGCGACCTAGAGAATGTG TGGCTTATGGGTGGTCTCAGTGTGCTGACTTCTGTGCCAGGGGGGCCACCGATGGTGTGTCTGCTGTGTG CTAGCAAAGGCCTCCATGAGCTGGTTTTTTGCCAAGTCTGCTGCGATCCTTTCCACCCCTTCTGTCTGGA GGAGGCTGAGCGGCCCTCACCTCAGCATCGTGACACCTGGTGCTGCAGGCGCTGCAAGTTCTGCCATGTT TGTGGGCGCAAAGGCCGAGGGTCCAAGCACCTCCTGGAATGTGAACGCTGTCGTCATGCTTACCACCCAG CCTGCCTGGGACCCAGCTACCCAACCCGGGCCACGCGTAGACGGCGCCACTGGATCTGTTCAGCCTGTGT GCGCTGTAAGAGCTGTGGGGCAACTCCAGGCAAGAACTGGGATGTCGAGTGGTCTGGAGATTACAGCCTC TGCCCCAGGTGCACCGAGCTCTATGAAAAAGGGAACTACTGCCCAATTTGCACACGCTGCTATGAAGACA ATGACTATGAGAGCAAGATGATGCAGTGTGCACAGTGTGACCACTGGGTGCATGCCAAGTGTGAGGGACT CTCAGATGAAGACTACGAGATCCTTTCTGGACTGCCAGACTCAGTGTTGTACACGTGTGGGCCATGTGCT GGGGCGACACAGCCCCGCTGGCGAGAGGCCCTGAGTGGGGCCCTGCAGGGAGGCCTGCGTCAGGTGCTCC AGGGCCTGTTAAGCTCAAAGGTGGCAGGCCCATTGCTGCTGTGCACCCAGTGTGGGCAGGATGGGAAGCA GCTCCACCCAGGACCCTGTGATTTACAAGCTGTGGGAAAGCGCTTTGAGGAAGGTCTCTATAAGTCTGTG CACAGCTTCATGGAAGACGTAGTGGCCATCTTGATGAGGCATTCAGAAGAAGGAGAGACCCCAGAGCGCC GGGCTGGAAGCCAGATGAAAGGACTCCTACTGAAGCTGCTAGAGTCTGCGTTCTGCTGGTTTGACGCCCA TGATCCTAAGTACTGGCGACGGAGTACACGGCTGCCAAACGGAGTGCTCCCCAATGCTGTGTTGCCTCCA TCCTTGGACCATGTCTATGCTCAGTGGAGACAACAGGAATCAGAGACCCCAGAATCGGGGCAGCCTCCAG GGGATCCTTCAGCAGCTTTCCAGAGCAAGGACCCAGCTGCTTTCTCACACCTGGATGACCCTCGTCAGTG TGCACTCTGTCTCAAGTATGGGGATGCTGACTCTAAGGAGGCGGGGCGGCTCCTGTACATTGGGCAGAAT GAGTGGACACATGTCAATTGTGCCATTTGGTCAGCTGAAGTGTTTGAGGAGAATGATGGCTCCCTCAAGA ACGTCCATGCTGCTGTTGCACGTGGAAGGCAGATGCGCTGTGAGCTCTGCCTCAAGCCTGGAGCCACAGT TGGCTGCTGCCTGTCCTCATGTCTCAGCAACTTCCACTTCATGTGTGCCCGGGCCAGCTACTGCATCTTC CAGGATGACAAGAAGGTTTTCTGCCAGAAACACACAGACCTCCTAGATGGCAAGGAGATCGTGACCCCTG ATGGTTTTGATGTTCTTCGGCGAGTCTATGTGGACTTCGAGGGCATCAACTTCAAGCGAAAGTTCTTGAC TGGACTTGAACCAGATGTCATCAATGTACTCATTGGCTCCATCAGAATCAACTCCCTGGGCACCCTATCT GATCTCTCAGACTGTGAGGGTCGACTCTTCCCCATTGGCTACCAATGCTCCCGCCTGTATTGGAGCACAG TGGATGCTCGGAGGCGCTGCTGGTATCGGTGCCGAATTCTAGAGTATCGCCCTTGGGGGCCAAGGGAGGA GCCAGTGCACCTAGAAGCAGCAGAGGAGAACCAGACCATTGTGCATAGCCCAACCCCTTCCTCAGAGCCC CCCAATCATGATGATCTGCCAGATACAGATTCTCTGATCCCTGGAGATCCTGTACACCACTCCCCCATTC AGAATCTGGATCCCCCGCTTCGGACAGATTCAAGCAATGGCCCTCCTCCCACCCCTCGCTCCTTCTCAGG GGCTCGAATCAAAGTGCCCAACTACTCACCGTCTCGGAGGCCCTTAGGGGGAGTCTCTTTTGGTCCTCTG CCCTCTCCAGGAAGTCCATCTTCCCTGACCCACCACATCCCTACTGTGGGAGACTCGGACTTTCCAGCTC CCCCAAGGCGATCTCGGCGTCCTAGCCCCCTGGCCACCAGGCCACCACCATCCCGGAGGACCTCCTCTCC TCTCAGAACTTCCCCTCAGCTCAGGGTGCCCCTGTCTACCTCAGTCACAGCCCTCACACCTACCTCAGGG GAGCTGGCTCCCCCTGACCTGGCCCCATCTCCCCTTCCACCGTCTGAAGACCTGGGCCCAGACTTTGAAG ACATGGAGGTGGTGTCAGGACTAAGTGCTGCTGACCTGGACTTTGCAGCCAGCCTGCTGGGGACTGAGCC TTTCCAGGAAGAGATTGTGGCTGCTGGGGCAGTGGGGAGCAGCCAGGGAGGTCCAGGGGACAGCTCAGAG GAGGAGGCCAGCCCTACCACTCACTATGTCCACTTCCCTGTGACCGTGGTGTCTGGCCCTGCCCTGGCCC CCAGTTCCCTTGCTGGAGCTCCCCGAATCGAGCAGCTGGACGGAGTGGATGATGGCACAGACAGCGAGGC TGAGGCTGTCCAACAACCCCGGGGACAGGGGACTCCTCCTTCAGGACCAGGAGTGGGCCGAGGAGGGGTC CTTGGAGCAGCAGGCGACAGAGCCCAACCTCCTGAAGACCTGCCTTCAGAAATTGTGGATTTCGTGTTGA AGAACCTAGGGGGTCCTGGGGAGGGGGCTGCTGGCCCCAGAGAGGACTCTCTTCCTTCAGCACCTCCCTT GGCTAATGGCAGCCAACCCCCACAAAGCCTGTCCACTAGCCCAGCTGACCCCACCCGAACATTTGCTTGG CTCCCTGGAGCTCCAGGGGTCCGGGTATTGAGTCTGGGTCCTGCTCCAGAACCCCCCAAACCTGCCACAT CCAAGATCATCCTTGTCAACAAGCTGGGGCAAGTATTTGTGAAGATGGCAGGGGAGGGTGAGCCTGTAGC ACCCCCAGTAAAGCAGCCCCCTCTACCCCCCATCATTCCCCCAACAGCTCCCACCTCCTGGACTCTGCCC CCAGGACCCCTGCTCAGTGTGTTACCAGTGGTAGGGGTAGGAGTGGTCCGGCCTGCCCCACCCCCACCTC CACCCCCACTAACACTGGTGTTTAGCAGTGGGCCCCCCAGCCCACCACGCCAGGCCATTCGTGTCAAGAG GGTATCCACCTTCTCTGGCCGATCCCCTCCAGTTCCTCCCCCAAACAAAACTCCCCGGCTGGATGAAGAT GGAGAGTCCTTGGAGGATGCCCATCATGTGCCAGGGATCAGTGGCAGTGGGTTCAGTCGTGTTCGCATGA AAACGCCCACAGTCCGTGGAGTTCTGGACCTGAACAATCCTGGGGAGCAGCCTGAGGAGGAAAGCCCTGG GCGCCCCCAGGACCGGTGTCCTTTGCTGCCACTTGCAGAAGCTCCTTCCCAGGCCCTCGATGGTTCCTCA GACCTACTGTTTGAGTCCCAGTGGCATCACTATTCAGCAGGCGAGGCCTCAAGCTCTGAGGAAGAGCCTC CATCTCCAGAGGACAAAGAAAACCAGGTCCCCAAACGGGTTGGTCCACACCTGCGCTTTGAGATCAGCAG TGATGATGGCTTCAGTGTGGAGGCAGAGAGCTTGGAGGTGGCATGGAGAACTCTGATTGAGAAAGTGCAA GAAGCACGAGGGCATGCACGGCTCAGGCATCTCTCCTTTAGTGGAATGAGTGGAGCGCGGCTCCTGGGCA TCCACCACGATGCTGTCATCTTCCTGGCAGAGCAGCTCCCTGGGGCCCAGCGCTGCCAGCATTATAAGTT CCGCTACCACCAGCAGGGAGAGGGCCAGGAAGAGCCACCTCTGAATCCCCATGGGGCTGCTCGTGCTGAG GTCTATCTCCGGAAGTGTACCTTTGACATGTTCAACTTCCTGGCCTCCCAGCACCGTGTGCTCCCCGAGG GAGCCACCTGTGATGAAGAAGAGGATGAGGTGCAGCTTCGGTCCACCAGACGTGCCACCAGCCTGGAGCT TCCTATGGCCATGCGCTTTCGTCATCTCAAAAAGACATCCAAAGAGGCTGTGGGAGTCTACAGATCAGCT ATCCATGGACGAGGTCTGTTTTGTAAACGCAACATCGATGCGGGAGAGATGGTCATTGAGTACTCTGGCA TTGTCATTCGCTCTGTGCTGACTGACAAGCGAGAGAAGTTCTATGATGGGAAGGGTATTGGGTGCTACAT GTTCCGCATGGATGACTTTGATGTGGTGGACGCCACCATGCACGGCAATGCTGCCCGATTCATCAACCAC TCATGTGAGCCCAACTGCTTCTCTCGTGTCATCCATGTGGAAGGCCAGAAACACATTGTCATCTTTGCCT TGCGCCGCATCCTGCGAGGTGAGGAGCTCACCTATGACTACAAGTTTCCTATCGAGGATGCCAGCAACAA GCTGCCCTGCAACTGTGGCGCCAAGCGCTGCCGTCGCTTTCTTAACTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001290573
Insert Size	8169 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290573.1, NP_001277502.1
RefSeq Size	8484 bp
RefSeq ORF	8169 bp
Locus ID	75410
Gene Summary	Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2 (By similarity). Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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