Cacna1a (NM_001252059) Mouse Untagged Clone

CAT#: MC229730

Cacna1a (untagged) - Mouse calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (Cacna1a), transcript variant 2



  "NM_001252059" in other vectors (1)

CNY 22,330.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms alpha1A; APCA; BI; Caca1a; Cacnl1a4; Cav2.1; Ccha1a; EA2; FHM; HPCA; la; MHP; MHP1; nmf352
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229730 representing NM_001252059
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCGCTTTGGAGACGAGATGCCGGGCCGCTACGGCGCAGGCGGAGGAGGCTCAGGGCCGGCCGCCG
GGGTGGTCGTGGGCGCCGCGGGCGGCCGAGGAGCCGGGGGCAGCCGGCAGGGCGGGCAGCCCGGAGCGCA
GAGGATGTACAAGCAGTCGATGGCGCAGAGAGCGCGGACCATGGCCCTCTACAACCCCATCCCTGTCCGC
CAGAACTGCCTCACGGTCAACCGCTCCCTGTTCCTCTTCAGTGAAGACAACGTGGATGACACGGAACCAT
ACTTCATTGGAATCTTCTGTTTTGAGGCTGGAATTAAGATCGTGGCTCTTGGCTTTGCCTTCCACAAAGG
CTCCTACCTGAGGAATGGCTGGAACGTCATGGACTTTGTCGTGGTGCTAACAGGCATCTTGGCCACTGTC
GGGACGGAGTTTGACCTACGGACACTGAGGGCGGTTCGTGTGCTGCGGCCACTCAAGCTGGTGTCTGGAA
TCCCAAGTTTACAAGTCGTCCTGAAGTCAATCATGAAGGCGATGATCCCTCTGCTGCAGATCGGCCTCCT
CCTGTTTTTTGCAATCCTTATTTTTGCAATCATAGGGTTAGAATTTTATATGGGGAAATTTCATACCACC
TGCTTTGAAGAGGGGACAGACGACATCCAGGGTGAGTCGCCAGCTCCGTGTGGGACAGAGGAGCCTGCCC
GCACCTGCCCCAACGGGACCAAATGTCAGCCGTACTGGGAAGGGCCCAACAACGGCATCACTCAGTTCGA
CAACATCCTGTTTGCTGTGCTCACTGTTTTCCAGTGCATCACCATGGAAGGCTGGACTGATCTCCTCTAC
AATAGCAACGATGCCTCAGGGAACACTTGGAACTGGTTGTACTTCATCCCCCTCATCATCATCGGCTCCT
TTTTTATGCTGAACCTTGTTCTGGGTGTGCTGTCTGGGGAGTTTGCCAAAGAAAGGGAACGTGTGGAGAA
CCGGAGAGCTTTTCTGAAGCTCAGAAGACAACAGCAGATTGAACGTGAGCTCAATGGATACATGGAGTGG
ATCTCAAAAGCAGAAGAGGTGATTCTTGCGGAGGACGAGACAGACGTGGAGCAGAGGCACCCTTTTGATG
GAGCTCTTCGGAGAGCTACTCTGAAGAAAAGCAAGACAGACCTGCTCAACCCTGAGGAGGCCGAGGACCA
GCTGGCTGACATCGCGTCTGTGGGGTCTCCCTTCGCCAGAGCCAGCATCAAAAGTGCCAAGCTGGAGAAC
TCGACTTTTTTTCACAAGAAGGAGAGAAGAATGCGTTTCTACATCCGCCGCATGGTCAAAACTCAGGCCT
TCTACTGGACCGTGCTCAGTCTGGTAGCCCTCAACACGCTGTGCGTCGCCATTGTCCACTACAACCAGCC
CGAGTGGCTCTCCGACTTCCTCTACTATGCAGAATTCATTTTCTTAGGACTCTTTATGTCCGAAATGTTT
ATAAAAATGTACGGGCTCGGGACGCGGCCTTACTTCCACTCTTCCTTCAACTGCTTTGACTGTGGGGTCA
TCATTGGGAGCATCTTTGAAGTCATCTGGGCTGTCATCAAACCTGGTACATCCTTTGGAATCAGTGTGTT
ACGAGCTCTCAGGTTATTGCGAATTTTCAAAGTCACAAAGTACTGGGCATCTCTCAGAAACCTGGTTGTC
TCCCTCCTCAACTCCATGAAGTCCATCATAAGTCTGTTGTTCCTCCTTTTCCTGTTCATCGTTGTCTTTG
CCCTCCTGGGGATGCAGCTGTTCGGTGGCCAATTTAATTTTGATGAGGGGACTCCGCCCACCAACTTCGA
CACTTTTCCAGCAGCAATAATGACTGTGTTTCAGATCCTGACTGGCGAGGATTGGAATGAGGTCATGTAT
GATGGGATCAAGTCTCAGGGGGGCGTGCAGGGCGGCATGGTGTTCTCCATCTACTTCATCGTCCTCACCC
TCTTCGGGAACTACACCCTGCTGAACGTGTTCTTGGCCATCGCAGTGGACAACCTGGCCAATGCCCAGGA
GCTCACCAAGGATGAGCAAGAAGAGGAAGAGGCAGCCAATCAGAAGCTGGCTCTACAGAAAGCCAAGGAG
GTAGCAGAAGTGAGTCCCCTGTCTGCAGCCAACATGTCCATAGCCGTGAAGGAGCAGCAGAAGAACCAGA
AGCCTACCAAGTCGGTGTGGGAGCAGCGCACCAGCGAGATGCGCAAGCAGAACCTGCTGGCCAGCCGCGA
GGCGCTGTACGGGGACGCGGCTGAGCGCTGGCCCACCCCCTACGCGCGCCCGCTGCGGCCCGACGTGAAG
ACTCACTTGGACCGGCCGCTTGTGGTGGACCCGCAGGAGAACCGTAACAACAACACCAACAAGAGCCGTG
CGCCAGAAGCGCTGCGCCCGACCGCGCGGCCCCGCGAAAGCGCGCGCGACCCCGACGCGCGGCGCGCCTG
GCCCGGCAGCCCTGAGCGCGCCCCTGGACGAGAGGGCCCGTATGGGCGCGAGAGCGAGCCTCAGCAGCGC
GAGCACGCGCCGCCCCGCGAGCACGCACCCTGGGACGCGGACACCGAGCGCGCCAAAGCCGGGGACGCGC
CCCGCCGCCACACGCACCGACCTGTGGCGGAGGGCGAGCCTCGCCGCCACCGCGCCCGCCGCCGGCCCGG
GGACGAACCCGACGACAGGCCAGAGCGCAGGCCACGTCCCCGCGACGCCACTAGGCCGGCCCGCGCTGCA
GACGGCGAAGGCGACGATGGGGAGCGCAAGCGGCGACACCGACACGGGCCGCCGGCCCACGACGACAGGG
AGCGCAGACACCGACGCAGAAAAGAGAACCAGGGCTCTGGGGTCCCCGTGTCTGGTCCCAACTTGTCCAC
CACCAGGCCAATCCAGCAGGACCTGGGCCGCCAGGACCTGCCGCTGGCTGAGGATCTTGACAACATGAAG
AATAACAAGCTGGCCACCGGGGAACCTGCCAGTCCCCACGACAGCCTGGGCCACAGCGGCCTTCCCCCCA
GCCCCGCCAAAATCGGGAACAGCACCAACCCTGGTCCCGCCTTGGCCACCAATCCCCAGAACGCTGCCAG
CCGCAGGACGCCCAACAATCCGGGCAACCCGTCCAACCCCGGCCCCCCCAAGACTCCCGAGAACAGCCTT
ATCGTCACCAACCCCAGCAGCACCCAGCCCAACTCAGCGAAGACTGCCAGGAAACCCGAGCACATGGCGG
TGGAGATCCCCCCAGCCTGCCCGCCCCTCAACCACACCGTGGTCCAAGTAAACAAAAACGCCAACCCAGA
CCCACTGCCAAAGAAAGAGGAAGAGAAGAAAGAGGAAGAGGAGGCAGACCCCGGGGAGGACGGCCCAAAG
CCCATGCCGCCCTACAGCTCCATGTTCATCCTCTCCACCACCAACCCCCTTCGCCGGCTGTGCCATTACA
TCCTGAACCTGCGCTACTTCGAGATGTGCATCCTCATGGTCATTGCCATGAGCAGCATCGCTCTGGCCGC
CGAGGACCCGGTGCAGCCCAACGCACCCCGCAACAATGTGCTGCGATATTTTGACTATGTTTTTACAGGC
GTGTTTACCTTTGAGATGGTGATCAAGATGATCGACCTGGGCCTCGTCTTGCATCAGGGGGCCTATTTCC
GTGACCTATGGAACATTCTGGACTTCATAGTGGTCAGTGGGGCCCTGGTGGCCTTTGCCTTCACTGGCAA
TAGCAAAGGAAAGGACATTAACACCATTAAGTCTCTCCGAGTCCTCCGGGTGCTACGACCTCTAAAGACC
ATCAAGCGGCTGCCAAAGCTGAAGGCCGTGTTTGACTGCGTGGTGAACTCGCTCAAGAACGTCTTCAACA
TCCTCATTGTGTACATGCTGTTCATGTTCATCTTCGCTGTGGTAGCCGTGCAGCTCTTCAAGGGCAAGTT
CTTCCACTGCACGGACGAGTCCAAGGAGTTCGAGAGAGACTGTCGAGGCAAGTACCTCCTGTACGAGAAG
AACGAAGTGAAGGCCCGGGACCGCGAGTGGAAGAAGTACGAGTTCCACTACGACAACGTGCTCTGGGCTC
TGCTCACACTTTTCACAGTGTCCACAGGAGAGGGCTGGCCACAGGTCCTCAAGCACTCAGTGGATGCTAC
TTTTGAGAACCAGGGCCCCAGCCCCGGGTACCGCATGGAGATGTCCATCTTCTACGTGGTGTACTTTGTA
GTGTTTCCCTTCTTCTTTGTCAATATCTTCGTGGCCTTGATCATCATCACCTTTCAGGAGCAGGGAGACA
AGATGATGGAAGAGTACAGCCTGGAGAAAAATGAGAGGGCCTGCATCGACTTTGCCATCAGTGCCAAGCC
GCTGACCAGGCACATGCCCCAGAACAAGCAGAGCTTCCAGTATCGAATGTGGCAGTTCGTCGTGTCCCCA
CCCTTTGAGTACACCATCATGGCCATGATCGCTCTCAACACCATCGTGCTAATGATGAAGTTCTACGGAG
CCTCGGTGGCCTATGAAAACGCCCTTCGCGTGTTCAACATTGTCTTCACCTCCCTCTTCTCTCTCGAATG
TGTGCTCAAAGTCATGGCTTTTGGGATTCTGAATTATTTCCGCGATGCCTGGAACATCTTCGACTTTGTG
ACTGTTCTGGGCAGCATCACAGACATCCTCGTCACCGAGTTTGGGAATAACTTCATCAACCTGAGCTTTC
TCCGCCTCTTCCGTGCTGCCAGACTCATCAAACTCCTCCGCCAGGGTTACACCATCCGCATTCTCCTCTG
GACTTTCGTGCAGTCCTTCAAGGCCCTACCTTATGTCTGTCTGCTGATCGCCATGCTCTTCTTCATCTAC
GCCATCATCGGGATGCAGGTGTTTGGCAACATCGGCATTGACGGGGAAGACGAGGACAGCGACGAGGATG
AATTCCAAATCACGGAGCACAATAACTTCCGGACCTTCTTCCAAGCGCTCATGCTTCTCTTCCGGAGCGC
CACAGGAGAAGCGTGGCACAACATCATGCTCTCCTGCCTCAGCGGGAAGCCGTGCGATAAGAACTCTGGG
ATCCTCACGGCAGACTGCGGCAACGAGTTCGCGTACTTCTACTTTGTCTCGTTCATCTTCCTGTGCTCGT
TTCTGATGCTGAATCTCTTTGTCGCTGTCATCATGGACAACTTCGAGTACCTCACCCGAGATTCTTCCAT
CCTGGGCCCCCATCACTTGGATGAATACGTGCGTGTCTGGGCAGAGTATGACCCTGCTGCCTGCGGCCGC
ATTCACTATAAGGACATGTACAGTTTATTGCGAGTAATATCGCCCCCTCTCGGCTTAGGCAAGAAATGTC
CTCATAGGGTTGCTTGCAAGAGACTCCTGCGGATGGACCTGCCGGTAGCAGATGACAACACAGTTCACTT
CAACTCCACCCTGATGGCTCTGATCCGAACCGCCCTTGATATCAAAATTGCCAAGGGTGGAGCTGACAAG
CAGCAGATGGATGCAGAGCTTCGCAAGGAGATGATGGCCATTTGGCCCAACCTGTCTCAGAAGACCCTGG
ATCTGCTGGTCACACCTCACAAGTCCACGGACCTGACAGTGGGTAAGATCTACGCAGCCATGATGATCAT
GGAGTATTACCGGCAGAGCAAGGCCAAGAAACTGCAGGCCATGCGAGAGGAGCAGAACCGGACACCACTC
ATGTTCCAGCGCATGGAGCCTCCATCACCAACACAGGAGGGAGGACCCAGCCAGAATGCCCTTCCCTCCA
CTCAGCTGGACCCAGGAGGAGGCCTGATGGCTCACGAAGGCGGCATGAAGGAGAGCCCGTCCTGGGTGAC
CCAGCGGGCCCAAGAGATGTTCCAGAAGACTGGCACCTGGAGCCCAGAGCGAGGGCCACCCATCGACATG
CCTAACAGCCAGCCCAACTCCCAGTCTGTGGAGATGCGAGAAATGGGTACTGATGGCTACTCAGACAGCG
AACACTACCTCCCCATGGAAGGCCAGACCAGGGCCGCCTCCATGCCCCGCCTCCCAGCAGAGAACCAGAG
GAGAAGGGGCCGGCCACGTGGAAATGACCTCAGTACCATCTCTGATACCAGCCCCATGAAGCGCTCAGCC
TCTGTGCTGGGACCCAAAGCCCGGCGACTGGATGACTACTCACTAGAGCGGGTACCGCCTGAGGAGAACC
AACGGTACCACCAACGTCGCCGGGACCGTGGCCACCGCACCTCTGAGCGCTCTCTGGGCCGATACACTGA
TGTGGACACAGGTTTGGGGACAGATCTGAGCATGACCACCCAATCGGGTGACCTGCCCTCCAAAGATCGG
GACCAGGACCGGGGCCGACCCAAGGACCGGAAGCATCGGCCACACCACCACCACCACCACCATCATCATC
ATCCCCCGGCCCCCGACCGGGATCGCTACGCACAGGAGCGGCCGGACACCGGCCGGGCGCGGGCCCGGGA
GCAGCGCTGGTCCCGCTCGCCCAGCGAAGGTCGGGAGCACACGACACACAGACAGGGCAGTAGTTCCGTC
AGTGGAAGCCCAGCCCCCTCTACGTCCGGCACCAGCACGCCGCGGCGCGGCCGCCGCCAGCTCCCCCAGA
CCCCCTGCACCCCGCGGCCGCTTGTGTCCTACTCGCCCGCTCCGCGCAGGCCTGCGGCGCGCAGGATGGC
GGGTCCCGCAGCGCCCCCTGGCGGCTCGCCGCGGGGTTGCCGCCGTGCGCCGCGCTGGCCTGCGCATGCG
CCTGAGGGCCCGCGGCCGCGCGGTGCCGACTACACCGAGCCCGACAGCCCGCGCGAGCCGCCAGGGGGCG
CGCACGATCCCGCGCCGCGGTCGCCCAGGACTCCGCGCGCCGCGGGCTGCGCGTCACCGCGCCACGGCCG
CAGGCTGCCCAATGGTTACTACGCGGGGCACGGCGCGCCGCGGCCGCGCACAGCCCGCAGGGGCGCGCAC
GACGCGTACAGCGAGAGCGAGGACGACTGGTGCTAA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001252059
Insert Size 6966 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001252059.1, NP_001238988.1
RefSeq Size 7788 bp
RefSeq ORF 6966 bp
Locus ID 12286
Gene Summary Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents. P/Q-type calcium channels belong to the 'high-voltage activated' (HVA) group and are specifically blocked by the spider omega-agatoxin-IVA (AC P54282) (By similarity). They are however insensitive to dihydropyridines (DHP).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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