Cacna1c (NM_001255997) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229712 representing NM_001255997 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCGGGCCTTTGTTCAGCCATCCACACCCCCATACCAGCCACTCTCCAGTCACTCGTCTGAGGAAA CAGAGAGGAAGTTCAAGGGCAAGGTGGTACATGAAGCTCAACTCAACTGTTTCTACATTTCTCCTGGAGG TTCCAACTATGGGAGCCCACGCCCAGCTCATGCCAACATGAATGCCAATGCAGCTGCAGGACTTGCTCCC GAGCACATCCCTACTCCAGGGGCAGCACTGTCCTGGCAGGCAGCCATCGATGCCGCCCGGCAGGCCAAGC TCATGGGCAGTGCTGGCAACGCAACCATCTCTACCGTCAGTTCCACACAGCGGAAGCGGCAGCAGTATGG GAAACCCAAGAAGCAGGGGGGCACAACCGCCACACGGCCGCCCCGGGCTCTGCTGTGTCTGACCCTGAAG AACCCTATCCGGAGGGCGTGCATAAGCATTGTTGAATGGAAACCATTTGAAATCATTATTTTACTGACTA TTTTTGCCAATTGTGTGGCCTTAGCAATCTATATTCCCTTTCCGGAAGACGACTCCAACGCCACCAACTC CAACCTGGAACGAGTGGAGTATCTCTTCCTCATCATTTTTACCGTGGAAGCATTTTTAAAAGTAATTGCC TACGGACTTCTCTTCCACCCCAACGCTTACCTCCGCAATGGTTGGAATTTACTGGATTTTATAATCGTGG TTGTAGGGCTTTTTAGTGCAATTTTAGAACAAGCAACCAAAGCTGATGGGGCCAATGCTCTAGGAGGGAA AGGAGCTGGATTCGACGTGAAGGCACTGAGAGCGTTCCGCGTGCTCCGTCCACTGCGGCTAGTGTCCGGA GTCCCAAGTCTCCAGGTGGTCCTGAACTCCATCATCAAGGCCATGGTGCCTCTGCTGCACATTGCCCTTC TTGTGCTCTTCGTCATCATCATTTATGCTATTATCGGCCTGGAGCTCTTCATGGGAAAGATGCACAAGAC CTGCTACAACCAGGAGGGCATAATAGATGTTCCGGCAGAAGAGGATCCTTCCCCTTGTGCTTTGGAGACA GGCCATGGGCGACAGTGTCAGAACGGGACCGTGTGCAAACCCGGGTGGGATGGGCCCAAGCACGGCATCA CCAACTTCGACAACTTCGCCTTCGCCATGCTGACGGTGTTCCAGTGTATCACCATGGAGGGCTGGACAGA CGTGCTGTACTGGATGCAAGACGCTATGGGCTATGAGTTGCCCTGGGTGTATTTTGTCAGTCTGGTCATC TTTGGATCCTTTTTCGTTCTAAATCTGGTTCTCGGTGTTTTGAGCGGGGAGTTTTCCAAAGAGAGGGAGA AAGCCAAAGCCCGAGGAGATTTCCAGAAGCTTCGAGAGAAGCAGCAACTAGAAGAAGATCTCAAAGGCTA CCTGGACTGGATCACCCAGGCAGAAGACATTGACCCCGAGAATGAGGACGAGGGCATGGATGAAGACAAG CCTCGAAACATGAGCATGCCCACAAGTGAGACTGAGTCTGTCAACACTGAAAACGTGGCTGGAGGTGACA TCGAGGGAGAAAACTGTGGAGCCAGGCTTGCCCATCGGATCTCCAAATCCAAATTCAGCCGCTACTGGCG CAGGTGGAATCGATTCTGCAGAAGAAAATGCCGTGCAGCAGTTAAGTCCAACGTCTTCTACTGGCTCGTG ATCTTCCTGGTGTTCCTCAACACCCTCACCATTGCCTCCGAACATTACAACCAGCCTCACTGGCTCACAG AAGTGCAAGACACAGCCAATAAAGCCCTCCTGGCCCTTTTCACTGCAGAAATGCTCCTGAAGATGTACAG CCTGGGTCTTCAGGCCTATTTTGTGTCCCTCTTCAACCGCTTTGACTGTTTCATTGTGTGTGGGGGCATC CTGGAGACCATCCTGGTGGAGACGAAGATCATGTCTCCCCTGGGCATCTCTGTGCTGAGATGTGTGCGGT TGCTCAGGATCTTCAAGATCACCAGGTACTGGAATTCCTTGAGCAACCTTGTGGCATCCTTGCTGAACTC AGTGCGCTCCATTGCCTCCCTGCTGCTGCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATG CAGCTCTTTGGAGGGAAGTTCAATTTCGATGAGATGCAGACCCGTAGGAGCACGTTCGATAACTTCCCGC AGTCTCTCCTCACTGTGTTTCAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCAT GGCTTATGGCGGCCCCTCTTTTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGA AATTATATCCTACTGAATGTGTTCTTGGCCATTGCGGTGGACAACCTGGCTGATGCGGAGAGCCTGACCT CAGCCCAAAAGGAGGAGGAAGAAGAGAAGGAGAGGAAGAAGCTGGCCAGGACTGCCAGCCCAGAAAAGAA ACAGGAGGTGATGGAGAAGCCAGCCGTGGAGGAGAGCAAAGAGGAGAAAATTGAACTGAAATCCATTACA GCCGATGGAGAATCCCCACCCACTACCAAGATCAACATGGATGACCTCCAGCCCAGTGAAAACGAGGATA AGAGTCCCCACTCCAACCCAGACACTGCAGGGGAAGAGGATGAAGAGGAGCCAGAGATGCCTGTGGGGCC ACGCCCCCGGCCCCTGTCTGAGCTGCACCTTAAGGAAAAGGCAGTTCCCATGCCGGAAGCCAGTGCATTT TTCATCTTCAGCCCAAACAACAGGTTCCGCCTGCAGTGCCACCGTATTGTCAATGACACGATCTTCACCA ACCTCATCCTCTTCTTCATTCTGCTCAGCAGCATCTCTCTGGCTGCTGAGGACCCCGTCCAGCACACCTC CTTCAGGAACCATATTCTGTTTTATTTTGACATTGTTTTTACCACCATTTTCACCATTGAAATTGCTCTA AAGATGACTGCTTACGGGGCTTTCCTGCACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCTGGACC TGCTGGTGGTTAGCGTGTCCCTCATCTCCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAGATTTT ACGAGTGCTGCGAGTCCTCAGACCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAG TGCGTGTTTGTGGCCATCCGGACCATCGGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCATGTTCG CCTGCATTGGGGTCCAGCTCTTCAAGGGAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACGGAGGC AGAATGCAAGGGTAACTATATCACATACAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTCGAAGC TGGGAGAACAGCAAGTTTGACTTTGACAATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTCCACCT TCGAAGGGTGGCCAGAGCTGCTGTACCGCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATCTACAA CTACCGTGTGGAGATCTCCATCTTCTTCATCATCTATATCATCATCATTGCCTTCTTCATGATGAACATC TTCGTGGGTTTCGTCATTGTCACCTTCCAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCTGGACA AGAACCAGAGACAATGTGTGGAATATGCCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCA GCACCAGTACAAAGTGTGGTACGTGGTCAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCATCCTG CTCAACACCATCTGCCTGGCCATGCAGCACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATAC TCAACATGCTTTTCACCGGCCTCTTCACAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGCA CTATTTCTGTGATGCATGGAATACATTTGACGCCTTGATTGTTGTGGGTAGCATTGTTGATATAGCAATC ACCGAGGTACACCCAGCTGAACATACCCAATGCTCTCCCTCTATGAGTGCAGAGGAGAACTCCCGCATCT CCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGGTGAAGCTGCTGAGCCGCGGGGAAGGCATCCG AACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCTCTGCCCTATGTGGCTCTTTTGATTGTGATGCTG TTCTTTATCTATGCAGTGATTGGGATGCAGGTGTTTGGGAAGATTGCCCTGAATGACACCACAGAGATCA ATCGGAACAACAACTTCCAGACGTTCCCCCAGGCTGTGTTACTGCTGTTCAGGTGTGCCACCGGAGAGGC CTGGCAGGACATCATGCTGGCCTGCATGCCAGGCAAGAAGTGTGCCCCAGAGTCTGAGCCCAGCAACAGC ACGGAAGGGGAGACCCCCTGTGGCAGCAGCTTTGCTGTCTTCTACTTCATCAGCTTCTACATGCTCTGTG CCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGGACAACTTTGACTACCTGACTAGGGATTGGTC TATCCTCGGTCCCCATCACCTGGATGAATTCAAGAGAATCTGGGCCGAGTATGACCCTGAAGCCAAGGGT CGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGAATTCAGCCCCCACTGGGTTTTGGGAAATTGT GTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGAACATGCCTCTGAACAGCGATGGCACAGTCAT GTTCAATGCTACCCTGTTTGCCCTCGTCAGGACAGCCCTGAGGATCAAAACAGAAGGGAACCTAGAGCAA GCCAATGAGGAGCTTCGGGCCATCATCAAGAAAATCTGGAAGAGGACTAGCATGAAGCTGTTGGACCAGG TGGTGCCCCCTGCAGGCGATGACGAGGTCACAGTGGGCAAGTTCTATGCCACCTTCCTGATCCAAGAGTA CTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGTGGGCAAGCCCTCACAAAGGAATGCACTGTCC CTCCAGGCTGGCTTGCGCACCTTGCATGACATTGGGCCTGAGATCCGGCGGGCCATCTCTGGGGATCTGA CTGCTGAGGAGGAGTTGGACAAGGCTATGAAGGAGGCGGTGTCTGCTGCCTCCGAAGATGACATCTTCAG GAGGGCTGGAGGCCTGTTCGGCAACCATGTCACCTACTATCAGAGTGACAGCAGGGGCAACTTTCCTCAG ACGTTCGCCACCCAGCGCCCACTGCACATCAACAAGACAGGGAACAACCAAGCTGACACTGAGTCACCGT CCCATGAGAAGCTGGTGGACTCCACGTTCACCCCCAGCAGCTACTCATCCACGGGCTCCAATGCCAACAT CAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCATCCCGCTGGCTACTCCAGCACGGTCAGCACT GTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGAGTACAGGAGGCAGCATGGAAGCTCAGCTCTA AGAGGTGCCACTCCCGAGAGAGCCAGGGAGCCACGGTGAATCAGGAGATATTTCCAGATGAGACCCGCAG CGTAAGGATGAGTGAAGAAGCCGAGTACTGCAGTGAGCCCAGCCTGCTCTCCACAGATATGTTCTCCTAC CAGGAAGATGAACACCGACAACTGACCTGCCCAGAGGAGGACAAGAGGGAGATCCAGCCATCTCCAAAGA GGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCTCCTTCCATCTGGAATGTCTAAAGCGACAAAA GGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCCCTTGCATCTGGTTCATCATCAGGCATTGGCA GTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCTCCTACCACATTCCCCAGGCCGTGCCCCACAC CCCCTGTCACTCCAGGCAGCCGGGGCAGACCCCTACGGCCCATCCCTACCCTACGGCTGGAGGGGGCAGA GTCCAGCGAGAAACTCAACAGCAGCTTCCCATCCATCCACTGCAGCTCCTGGTCTGAGGAGACGACAGCC TGTAGTGGGAGCAGCAGCATGGCCCGGAGAGCCCGGCCCGTCTCCCTCACCGTGCCCAGCCAGGCTGGAG CTCCAGGGAGACAGTTCCATGGCAGTGCCAGCAGCCTGGTGGAAGCGGTCTTGATTTCAGAAGGACTGGG ACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACCCAGGAGCTGGCTGACGCCTGCGACATGACA ATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGTGGGGGCGCCCAGCAGAGCCCCAACGGCACCC TCTTACCTTTTGTGAACTGCAGGGACCCGGGGCAGGACAGGGCTGTGGCCCCAGAGGACGAGAGCTGCGC ATATGCCCTGGGGCGAGGCCGGAGCGAGGAGGCGCTCGCGGACAGCAGGTCCTACGTCAGCAACCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001255997
Insert Size	6510 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001255997.2, NP_001242926.1
RefSeq Size	13340 bp
RefSeq ORF	6510 bp
Locus ID	12288
Gene Summary	Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 5, which is the same length as isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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