Scn9a (NM_001290674) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mKIAA4197; Nav1.7; PN1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229689 representing NM_001290674 Red=Cloning site Blue=ORF Orange=Stop codon CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGCGATGTTGCCTCCGCCAGGACCTCAGAGCTTCGTTCACTTCACAAAACAGTCCCTTGCCCTCATTG AACAACGCATTTCTGAAGAAAAAGCCAAGGGACACAAAGATGAAAAGAAAGATGATGAGGAAGAAGGTCC CAAGCCCAGTAGTGACTTGGAAGCAGGTAAACAGCTACCCTTCATCTACGGAGACATTCCCCCGGGAATG GTGTCAGAGCCCCTGGAGGACCTGGACCCATACTATGCAGACAAAAAAACTTTTATAGTATTGAACAAAG GGAAAGCAATCTTCCGTTTCAACGCCACCCCTGCTTTGTACATGCTGTCTCCCTTCAGTCCTCTCAGAAG AATATCTATTAAGATTTTAGTGCACTCCTTATTCAGCATGCTAATCATGTGCACAATTCTGACAAACTGC ATATTCATGACCATGAGCAACCCTCCAGATTGGACCAAAAACGTAGAGTACACTTTTACTGGGATATATA CTTTTGAATCACTCATAAAAATCCTTGCAAGAGGCTTTTGCGTGGGCGAATTCACCTTCCTCCGTGACCC TTGGAACTGGCTGGACTTTGTTGTCATTGTTTTTGCGTATTTAACAGAATTTGTAAACCTAGGCAATGTT TCAGCTCTTCGAACTTTCAGAGTCTTGAGAGCTTTGAAAACTATTTCTGTAATTCCAGGACTAAAAACCA TCGTGGGGGCCCTGATCCAATCAGTGAAGAAGCTCTCTGACGTCATGATCCTCACTGTGTTCTGTCTCAG TGTGTTCGCACTAATTGGACTACAACTGTTTATGGGCAACTTGAAGCATAAATGTTTCCGGAAGGACCTT GAGCAGAATGAAACATTAGAAAGCATCATGAGTACTGCTGAGAGTGAAGAAGAATTGAAAAGATATTTTT ATTACTTGGAGGGATCCAAAGATGCTCTTCTTTGCGGTTTCAGCACAGATTCAGGGCAGTGTCCTGAAGG GTACGAGTGTGTGACAGCTGGCAGAAACCCAGATTATGGCTACACAAGCTTTGACACGTTCGGCTGGGCC TTCTTGGCCTTGTTTCGGCTAATGACTCAGGACTACTGGGAGAACCTTTATCAACAGACACTGCGTGCTG CTGGCAAAACCTACATGATTTTCTTTGTCGTGGTGATATTTCTGGGATCCTTTTACCTGATAAACTTGAT CCTGGCTGTGGTAGCCATGGCGTACGAGGAACAGAACCAGGCCAACATCGAAGAAGCTAAACAGAAAGAG TTAGAATTTCAGCAGATGTTAGACCGACTCAAAAAAGAGCAGGAAGAAGCCGAGGCGATCGCTGCAGCCG CTGCTGAGTACACGAGTTTAGGGCGGAGCAGGATTATGGGACTCTCTGAGAGCTCTTCAGAAACCTCCAG GCTGAGCTCAAAGAGTGCCAAGGAGAGAAGAAACCGAAGAAAGAAAAAAAAACAGAAGCTGTCCAGTGGC GAGGAAAAGGGTGATGATGAGAAGCTGTCCAAGTCAGGGTCAGAGGAAAGCATCCGAAAGAAAAGCTTCC ATCTCGGCGTGGAAGGGCACCACCGGGCCAGGGAAAAGAGGCTGTCCACCCCCAACCAGTCACCACTCAG CATTCGTGGGTCCTTGTTTTCTGCCAGGCGCAGCAGCAGAACAAGTCTCTTCAGTTTTAAGGGGCGAGGA AGAGATCTGGGATCTGAAACGGAATTTGCTGATGATGAGCATAGCATTTTTGGAGACAACGAGAGCAGAA GGGGTTCACTATTTGTACCCCATAGACCCCGGGAGCGGCGCAGCAGTAACATCAGCCAGGCCAGTAGGTC CCCACCAGTGCTGCCGGTGAACGGGAAGATGCACAGTGCAGTGGACTGCAATGGCGTGGTGTCGCTTGTT GATGGACCCTCAGCCCTCATGCTCCCCAATGGACAGCTTCTTCCAGAGGTGATAATAGATAAGGCAACTT CCGACGACAGCGGCACAACTAATCAGATGCGTAAAAAAAGGCTCTCTAGTTCTTACTTTTTGTCTGAGGA CATGCTGAATGACCCACATCTCAGGCAAAGGGCCATGAGCAGAGCAAGCATTCTAACCAACACAGTAGAA GAACTTGAAGAATCTAGACAAAAATGTCCACCATGGTGGTACAGATTTGCTCACACATTTTTAATCTGGA ATTGTTCTCCATATTGGATAAAATTCAAAAAGTTCATCTATTTTATTGTAATGGATCCTTTTGTAGATCT TGCAATTACCATTTGCATAGTTTTAAACACCTTGTTTATGGCTATGGAGCACCATCCAATGACGGATGAA TTCAAAAATGTACTTGCAGTCGGGAACCTGGTCTTCACAGGGATCTTCGCAGCTGAAATGGTACTGAAGT TAATAGCCATGGATCCCTATGAATATTTCCAAGTAGGGTGGAATATTTTTGACAGCCTGATTGTGACGTT GAGTTTGGTGGAGCTTTTCCTAGCAGATGTGGAAGGATTATCAGTCCTGCGGTCCTTTAGATTGCTGCGA GTCTTCAAGTTGGCAAAATCCTGGCCCACACTGAATATGCTCATTAAGATCATCGGCAACTCGGTGGGCG CACTGGGCAACCTGACCCTGGTGCTGGCCATCATCGTCTTCATTTTTGCCGTGGTCGGCATGCAGCTGTT TGGAAAGAGCTACAAGGAGTGTGTTTGCAAGATCAACGAGAACTGCAAGCTCCCACGCTGGCACATGAAC GACTTCTTCCACTCCTTCCTGATCGTGTTCCGTGTGCTGTGTGGGGAGTGGATAGAGACCATGTGGGACT GCATGGAGGTTGCGGGCCAGACCATGTGCCTTATTGTTTACATGATGGTCATGGTGATTGGGAACCTTGT GGTCCTGAACCTGTTTCTGGCTTTATTACTGAGTTCCTTTAGTTCTGACAATCTTACAGCAATTGAAGAA GACACCGACGCAAACAACCTCCAGATTGCAGTGGCCAGAATTAAAAGAGGGATCAATTATGTGAAACAGA CCCTGCGTGAATTCATTCTAAAGTCATTTTCCAAAAAGCCAAAGGGCTCCAAGGACACAAAACGAACAGC GGATCCCAACAACAAAAGAGAAAACTATATCTCAAACCGTACCCTTGCGGAGATAAGCAAAGATCACAAT TTCCTCAAAGAAAAGGATAAGATCAGTGGTTTTAGCAGCAGTCTAGACAAAAGCTTTATGGATGAAAACG ATTACCAGTCCTTTATTCATAATCCCAGCCTCACAGTGACAGTGCCCATTGCACCTGGGGAGTCTGATTT GGAGAATATGAACACAGAAGAGCTTAGCAGTGACTCAGATAGTGACTACAGCAAAGAGAGACGGAACCGA TCAAGTTCTTCAGAGTGCAGCACAGTTGATAACCCTCTGCCAGGAGAAGAGGAGGCAGAAGCTGAGCCTA TCAATGCAGATGAGCCCGAAGCCTGTTTTACAGATGGCTGTGTGAGGAGATTTCCATGCTGCCAAGTTAA CATAGACTCCGGGAAAGGGAAAGTTTGGTGGACCATCCGGAAGACCTGCTACAGGATAGTGGAGCACAGC TGGTTTGAAAGCTTCATTGTTCTCATGATCCTGCTCAGCAGTGGAGCTCTGGCTTTTGAGGATATATATA TTGAAAAGAAAAAGACCATTAAGATTATCCTGGAGTATGCTGACAAGATATTCACCTACATCTTCATTCT GGAAATGCTTCTAAAATGGGTGGCATACGGGTATAAAACATATTTCACTAATGCCTGGTGTTGGCTGGAC TTCTTAATTGTTGATGTGTCTCTAGTTACTTTAGTAGCCAACACTCTTGGCTACTCAGACCTTGGCCCCA TTAAATCTCTACGGACACTGAGGGCCCTAAGACCCCTAAGAGCTTTGTCTAGATTTGAAGGAATGAGGGT AGTGGTCAACGCACTCATAGGAGCAATCCCTTCCATCATGAATGTGCTTCTTGTGTGCCTTATATTCTGG CTAATATTTAGCATCATGGGAGTCAATCTGTTTGCTGGCAAGTTCTATGAGTGTGTTAACACCACAGATG GCTCACGATTTTCTGTATCTCAAGTTGCAAACCGTTCTGAGTGTTTTGCCCTGATGAATGTTAGTGGAAA TGTGCGATGGAAAAACCTGAAAGTAAACTTCGATAACGTTGGACTTGGTTACCTGTCGCTGCTTCAAGTT GCAACGTTCAAGGGCTGGATGGATATTATGTATGCAGCAGTTGACTCTGTTAATGTAAATGCACAACCAA TATATGAATACAACCTCTACATGTACATTTATTTTGTCATCTTCATCATCTTTGGCTCATTCTTCACTTT GAACTTGTTCATTGGTGTCATCATCGATAATTTCAACCAACAGAAGAAGAAGCTTGGAGGTCAAGATATC TTTATGACAGAAGAACAGAAGAAATACTATAATGCAATGAAGAAGCTGGGGTCCAAGAAACCACAAAAAC CAATTCCGAGGCCAGGGAACAAATTCCAAGGATGCATATTTGACTTAGTGACAAACCAAGCTTTTGATAT CACCATCATGGTTCTTATCTGCCTCAATATGGTAACCATGATGGTAGAAAAAGAGGGGCAAACTGACTAC ATGAGTTTTGTGCTATACTGGATCAACGTGGTCTTCATCATCCTGTTCACTGGGGAGTGTGTGCTGAAGC TGATCTCTCTCAGGCATTACTACTTCACTGTGGGATGGAACATTTTTGATTTTGTGGTAGTGATCCTCTC CATTGTAGGAATGTTTCTCGCTGAGATGATAGAGAAGTATTTCGTGTCTCCTACCCTGTTCCGAGTCATT CGCCTGGCCAGGATTGGACGAATCCTACGCCTGATCAAAGGCGCCAAGGGGATCCGCACGCTGCTCTTTG CTCTGATGATGTCCCTTCCTGCGCTGTTCAACATCGGCCTCCTGCTTTTCCTCGTCATGTTCATCTACGC CATCTTTGGGATGTCCAACTTTGCCTACGTTAAAAAGGAAGCTGGAATTAATGACATGTTCAACTTTGAG ACCTTCGGCAACAGCATGATCTGCCTGTTCCAAATCACCACCTCTGCGGGCTGGGATGGACTGCTGGCCC CCATCCTCAACAGTGCACCTCCTGACTGTGACCCAAAAAAGGTTCACCCAGGAAGTTCAGTGGAAGGGGA CTGTGGAAATCCATCTGTGGGAATTTTCTACTTTGTCAGCTACATCATCATATCCTTCCTGGTTGTGGTG AACATGTACATTGCTGTCATCCTGGAGAACTTCAGCGTTGCCACAGAAGAAAGTACTGAGCCCCTGAGTG AGGACGACTTTGAGATGTTCTACGAAGTCTGGGAGAAGTTCGACCCTGACGCCACCCAGTTCATAGAGTT CTGCAAGCTCTCTGACTTTGCAGCAGCCCTGGATCCTCCCCTCCTCATCGCAAAGCCAAACAAAGTCCAG CTCATTGCCATGGACCTGCCCATGGTGAGTGGAGACCGCATCCACTGCCTGGACATCTTATTTGCTTTTA CAAAGCGGGTCCTGGGCGAGAGCGGAGAGATGGATTCCCTTCGTTCACAGATGGAAGAAAGGTTTATGTC AGCCAATCCTTCTAAAGTGTCCTATGAGCCCATCACAACCACACTGAAGCGAAAACAAGAGGATGTATCT GCGACTATCATTCAGCGTGCTTACAGACGGTACCGCCTTAGGCAAAACGTCAAGAATATATCAAGTATAT ATATAAAAGATGGAGACAGAGATGACGATTTGCCCAATAAAGAAGATATAGTTTTTGATAATGTTAACGA GAACTCAAGTCCAGAAAAGACAGATGCAACAGCCTCTACCATCTCTCCACCTTCCTATGACAGTGTCACA AAGCCAGATCAAGAGAAATATGAAACAGACAAAACGGAGAAGGAAGACAAAGAGAAAGACGAAAGCAGGA AATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-MluI
ACCN	NM_001290674
Insert Size	5955 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290674.1, NP_001277603.1
RefSeq Size	9865 bp
RefSeq ORF	5955 bp
Locus ID	20274
UniProt ID	Q62205
Gene Summary	Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:15123669). It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:15314237).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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