Scn9a (NM_001290675) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mKIAA4197; Nav1.7; PN1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229687 representing NM_001290675 Red=Cloning site Blue=ORF Orange=Stop codon CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGCGATGTTGCCTCCGCCAGGACCTCAGAGCTTCGTTCACTTCACAAAACAGTCCCTTGCCCTCATTG AACAACGCATTTCTGAAGAAAAAGCCAAGGGACACAAAGATGAAAAGAAAGATGATGAGGAAGAAGGTCC CAAGCCCAGTAGTGACTTGGAAGCAGGTAAACAGCTACCCTTCATCTACGGAGACATTCCCCCGGGAATG GTGTCAGAGCCCCTGGAGGACCTGGACCCATACTATGCAGACAAAAAAACTTTTATAGTATTGAACAAAG GGAAAGCAATCTTCCGTTTCAACGCCACCCCTGCTTTGTACATGCTGTCTCCCTTCAGTCCTCTCAGAAG AATATCTATTAAGATTTTAGTGCACTCCTTATTCAGCATGCTAATCATGTGCACAATTCTGACAAACTGC ATATTCATGACCATGAGCAACCCTCCAGATTGGACCAAAAACGTAGAGTACACTTTTACTGGGATATATA CTTTTGAATCACTCATAAAAATCCTTGCAAGAGGCTTTTGCGTGGGCGAATTCACCTTCCTCCGTGACCC TTGGAACTGGCTGGACTTTGTTGTCATTGTTTTTGCATATGTGACAGAGTTTGTGGACCTGGGCAATGTC TCAGCGTTGAGAACATTCAGAGTTCTCCGAGCATTGAAAACAATATCAGTCATTCCAGGACTAAAAACCA TCGTGGGGGCCCTGATCCAATCAGTGAAGAAGCTCTCTGACGTCATGATCCTCACTGTGTTCTGTCTCAG TGTGTTCGCACTAATTGGACTACAACTGTTTATGGGCAACTTGAAGCATAAATGTTTCCGGAAGGACCTT GAGCAGAATGAAACATTAGAAAGCATCATGAGTACTGCTGAGAGTGAAGAAGAATTGAAAAGATATTTTT ATTACTTGGAGGGATCCAAAGATGCTCTTCTTTGCGGTTTCAGCACAGATTCAGGGCAGTGTCCTGAAGG GTACGAGTGTGTGACAGCTGGCAGAAACCCAGATTATGGCTACACAAGCTTTGACACGTTCGGCTGGGCC TTCTTGGCCTTGTTTCGGCTAATGACTCAGGACTACTGGGAGAACCTTTATCAACAGACACTGCGTGCTG CTGGCAAAACCTACATGATTTTCTTTGTCGTGGTGATATTTCTGGGATCCTTTTACCTGATAAACTTGAT CCTGGCTGTGGTAGCCATGGCGTACGAGGAACAGAACCAGGCCAACATCGAAGAAGCTAAACAGAAAGAG TTAGAATTTCAGCAGATGTTAGACCGACTCAAAAAAGAGCAGGAAGAAGCCGAGGCGATCGCTGCAGCCG CTGCTGAGTACACGAGTTTAGGGCGGAGCAGGATTATGGGACTCTCTGAGAGCTCTTCAGAAACCTCCAG GCTGAGCTCAAAGAGTGCCAAGGAGAGAAGAAACCGAAGAAAGAAAAAAAAACAGAAGCTGTCCAGTGGC GAGGAAAAGGGTGATGATGAGAAGCTGTCCAAGTCAGGGTCAGAGGAAAGCATCCGAAAGAAAAGCTTCC ATCTCGGCGTGGAAGGGCACCACCGGGCCAGGGAAAAGAGGCTGTCCACCCCCAACCAGTCACCACTCAG CATTCGTGGGTCCTTGTTTTCTGCCAGGCGCAGCAGCAGAACAAGTCTCTTCAGTTTTAAGGGGCGAGGA AGAGATCTGGGATCTGAAACGGAATTTGCTGATGATGAGCATAGCATTTTTGGAGACAACGAGAGCAGAA GGGGTTCACTATTTGTACCCCATAGACCCCGGGAGCGGCGCAGCAGTAACATCAGCCAGGCCAGTAGGTC CCCACCAGTGCTGCCGGTGAACGGGAAGATGCACAGTGCAGTGGACTGCAATGGCGTGGTGTCGCTTGTT GATGGACCCTCAGCCCTCATGCTCCCCAATGGACAGCTTCTTCCAGAGGGCACAACTAATCAGATGCGTA AAAAAAGGCTCTCTAGTTCTTACTTTTTGTCTGAGGACATGCTGAATGACCCACATCTCAGGCAAAGGGC CATGAGCAGAGCAAGCATTCTAACCAACACAGTAGAAGAACTTGAAGAATCTAGACAAAAATGTCCACCA TGGTGGTACAGATTTGCTCACACATTTTTAATCTGGAATTGTTCTCCATATTGGATAAAATTCAAAAAGT TCATCTATTTTATTGTAATGGATCCTTTTGTAGATCTTGCAATTACCATTTGCATAGTTTTAAACACCTT GTTTATGGCTATGGAGCACCATCCAATGACGGATGAATTCAAAAATGTACTTGCAGTCGGGAACCTGGTC TTCACAGGGATCTTCGCAGCTGAAATGGTACTGAAGTTAATAGCCATGGATCCCTATGAATATTTCCAAG TAGGGTGGAATATTTTTGACAGCCTGATTGTGACGTTGAGTTTGGTGGAGCTTTTCCTAGCAGATGTGGA AGGATTATCAGTCCTGCGGTCCTTTAGATTGCTGCGAGTCTTCAAGTTGGCAAAATCCTGGCCCACACTG AATATGCTCATTAAGATCATCGGCAACTCGGTGGGCGCACTGGGCAACCTGACCCTGGTGCTGGCCATCA TCGTCTTCATTTTTGCCGTGGTCGGCATGCAGCTGTTTGGAAAGAGCTACAAGGAGTGTGTTTGCAAGAT CAACGAGAACTGCAAGCTCCCACGCTGGCACATGAACGACTTCTTCCACTCCTTCCTGATCGTGTTCCGT GTGCTGTGTGGGGAGTGGATAGAGACCATGTGGGACTGCATGGAGGTTGCGGGCCAGACCATGTGCCTTA TTGTTTACATGATGGTCATGGTGATTGGGAACCTTGTGGTCCTGAACCTGTTTCTGGCTTTATTACTGAG TTCCTTTAGTTCTGACAATCTTACAGCAATTGAAGAAGACACCGACGCAAACAACCTCCAGATTGCAGTG GCCAGAATTAAAAGAGGGATCAATTATGTGAAACAGACCCTGCGTGAATTCATTCTAAAGTCATTTTCCA AAAAGCCAAAGGGCTCCAAGGACACAAAACGAACAGCGGATCCCAACAACAAAAGAGAAAACTATATCTC AAACCGTACCCTTGCGGAGATAAGCAAAGATCACAATTTCCTCAAAGAAAAGGATAAGATCAGTGGTTTT AGCAGCAGTCTAGACAAAAGCTTTATGGATGAAAACGATTACCAGTCCTTTATTCATAATCCCAGCCTCA CAGTGACAGTGCCCATTGCACCTGGGGAGTCTGATTTGGAGAATATGAACACAGAAGAGCTTAGCAGTGA CTCAGATAGTGACTACAGCAAAGAGAGACGGAACCGATCAAGTTCTTCAGAGTGCAGCACAGTTGATAAC CCTCTGCCAGGAGAAGAGGAGGCAGAAGCTGAGCCTATCAATGCAGATGAGCCCGAAGCCTGTTTTACAG ATGGCTGTGTGAGGAGATTTCCATGCTGCCAAGTTAACATAGACTCCGGGAAAGGGAAAGTTTGGTGGAC CATCCGGAAGACCTGCTACAGGATAGTGGAGCACAGCTGGTTTGAAAGCTTCATTGTTCTCATGATCCTG CTCAGCAGTGGAGCTCTGGCTTTTGAGGATATATATATTGAAAAGAAAAAGACCATTAAGATTATCCTGG AGTATGCTGACAAGATATTCACCTACATCTTCATTCTGGAAATGCTTCTAAAATGGGTGGCATACGGGTA TAAAACATATTTCACTAATGCCTGGTGTTGGCTGGACTTCTTAATTGTTGATGTGTCTCTAGTTACTTTA GTAGCCAACACTCTTGGCTACTCAGACCTTGGCCCCATTAAATCTCTACGGACACTGAGGGCCCTAAGAC CCCTAAGAGCTTTGTCTAGATTTGAAGGAATGAGGGTAGTGGTCAACGCACTCATAGGAGCAATCCCTTC CATCATGAATGTGCTTCTTGTGTGCCTTATATTCTGGCTAATATTTAGCATCATGGGAGTCAATCTGTTT GCTGGCAAGTTCTATGAGTGTGTTAACACCACAGATGGCTCACGATTTTCTGTATCTCAAGTTGCAAACC GTTCTGAGTGTTTTGCCCTGATGAATGTTAGTGGAAATGTGCGATGGAAAAACCTGAAAGTAAACTTCGA TAACGTTGGACTTGGTTACCTGTCGCTGCTTCAAGTTGCAACGTTCAAGGGCTGGATGGATATTATGTAT GCAGCAGTTGACTCTGTTAATGTAAATGCACAACCAATATATGAATACAACCTCTACATGTACATTTATT TTGTCATCTTCATCATCTTTGGCTCATTCTTCACTTTGAACTTGTTCATTGGTGTCATCATCGATAATTT CAACCAACAGAAGAAGAAGCTTGGAGGTCAAGATATCTTTATGACAGAAGAACAGAAGAAATACTATAAT GCAATGAAGAAGCTGGGGTCCAAGAAACCACAAAAACCAATTCCGAGGCCAGGGAACAAATTCCAAGGAT GCATATTTGACTTAGTGACAAACCAAGCTTTTGATATCACCATCATGGTTCTTATCTGCCTCAATATGGT AACCATGATGGTAGAAAAAGAGGGGCAAACTGACTACATGAGTTTTGTGCTATACTGGATCAACGTGGTC TTCATCATCCTGTTCACTGGGGAGTGTGTGCTGAAGCTGATCTCTCTCAGGCATTACTACTTCACTGTGG GATGGAACATTTTTGATTTTGTGGTAGTGATCCTCTCCATTGTAGGAATGTTTCTCGCTGAGATGATAGA GAAGTATTTCGTGTCTCCTACCCTGTTCCGAGTCATTCGCCTGGCCAGGATTGGACGAATCCTACGCCTG ATCAAAGGCGCCAAGGGGATCCGCACGCTGCTCTTTGCTCTGATGATGTCCCTTCCTGCGCTGTTCAACA TCGGCCTCCTGCTTTTCCTCGTCATGTTCATCTACGCCATCTTTGGGATGTCCAACTTTGCCTACGTTAA AAAGGAAGCTGGAATTAATGACATGTTCAACTTTGAGACCTTCGGCAACAGCATGATCTGCCTGTTCCAA ATCACCACCTCTGCGGGCTGGGATGGACTGCTGGCCCCCATCCTCAACAGTGCACCTCCTGACTGTGACC CAAAAAAGGTTCACCCAGGAAGTTCAGTGGAAGGGGACTGTGGAAATCCATCTGTGGGAATTTTCTACTT TGTCAGCTACATCATCATATCCTTCCTGGTTGTGGTGAACATGTACATTGCTGTCATCCTGGAGAACTTC AGCGTTGCCACAGAAGAAAGTACTGAGCCCCTGAGTGAGGACGACTTTGAGATGTTCTACGAAGTCTGGG AGAAGTTCGACCCTGACGCCACCCAGTTCATAGAGTTCTGCAAGCTCTCTGACTTTGCAGCAGCCCTGGA TCCTCCCCTCCTCATCGCAAAGCCAAACAAAGTCCAGCTCATTGCCATGGACCTGCCCATGGTGAGTGGA GACCGCATCCACTGCCTGGACATCTTATTTGCTTTTACAAAGCGGGTCCTGGGCGAGAGCGGAGAGATGG ATTCCCTTCGTTCACAGATGGAAGAAAGGTTTATGTCAGCCAATCCTTCTAAAGTGTCCTATGAGCCCAT CACAACCACACTGAAGCGAAAACAAGAGGATGTATCTGCGACTATCATTCAGCGTGCTTACAGACGGTAC CGCCTTAGGCAAAACGTCAAGAATATATCAAGTATATATATAAAAGATGGAGACAGAGATGACGATTTGC CCAATAAAGAAGATATAGTTTTTGATAATGTTAACGAGAACTCAAGTCCAGAAAAGACAGATGCAACAGC CTCTACCATCTCTCCACCTTCCTATGACAGTGTCACAAAGCCAGATCAAGAGAAATATGAAACAGACAAA ACGGAGAAGGAAGACAAAGAGAAAGACGAAAGCAGGAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-MluI
ACCN	NM_001290675
Insert Size	5922 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290675.1, NP_001277604.1
RefSeq Size	9832 bp
RefSeq ORF	5922 bp
Locus ID	20274
Gene Summary	Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:15123669). It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:15314237).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon but contains an alternate in-frame exon in the 5' coding region, and also contains an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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