Scn10a (NM_001205321) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Nav1.8; PN3; SNS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229685 representing NM_001205321 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCCCTTTGGGTCCGTGGGAACTACCAACTTCAGACGGTTCACTCCAGAGTCGCTGGCAGAGA TCGAGAAGCAGATCGCTGCCCACCGCGCCGCCAAGAAGGGCAGACCTAAGCAAAGAGGACAGAAGGACAA GAGTGAGAAGCCCAGGCCTCAGTTGGACTTGAAGGCCTGTAACCAGCTGCCCAGGTTCTATGGCGAGCTC CCAGCAGAGCTGGTCGGGGAGCCCCTGGAGGACCTGGATCCTTTCTACAGCACACACCGGACATTCATAG TGTTGGATAAAAGCAGGACCATTTCCAGATTCAGTGCCACTTGGGCTCTGTGGCTCTTCAGTCCCTTCAA CCTGATCAGAAGAACAGCCATCAAAGTGTCCGTCCACTCCTGGTTCTCCATATTTATCACTGTCACTATT TTGGTCAACTGTGTGTGCATGACCCGAACTGATCTTCCAGAGAAACTCGAGTATGCCTTCACTGTTGTTT ACACCTTCGAGGCTCTGATAAAGATACTGGCAAGAGGGTTTTGTCTAAATGAATTCACTTATCTTCGAGA TCCCTGGAACTGGCTGGACTTCAGTGTCATTACCCTGGCGTATGTGGGTGCAGCGATAGACCTCCGAGGA ATCTCAGGCCTGCGGACATTCCGAGTTCTCAGGGCCCTGAAGACTGTTTCTGTGATCCCAGGACTGAAGG TCATCGTGGGAGCCCTGATCCACTCAGTGAGGAAGCTGGCCGACGTGACCATCCTCACAGTCTTCTGCCT GAGTGTCTTTGCCTTGGTGGGCCTGCAGCTCTTCAAGGGGAACCTCAAGAATAAATGCATTAAGAACGGC ACAGATCCGCACAAGGCTGACAATCTCTCATCTGAAATGGCAGGAGACATCTTCATCAAGCCCGGTACTA CGGATCCCTTGTTGTGTGGCAATGGATCTGATGCTGGCCACTGCCCTAATGATTATGTCTGCCGGAAAAC TTCTGACAACCCGGATTTTAACTACACCAGCTTTGATTCCTTCGCGTGGGCGTTCCTCTCACTGTTCCGT CTCATGACGCAGGACTCCTGGGAACGGCTGTACCAGCAGACACTCCGGGCTTCCGGGAAAATGTACATGG TCTTTTTTGTGCTGGTCATCTTCCTTGGATCATTCTACCTGGTCAATTTGATCTTGGCTGTGGTCACCAT GGCATATGAGGAACAGAGCCAGGCAACAATTGCAGAAATCGAAGCCAAGGAGAAGAAGTTCAAGGAAGCC CTCGAGGTGCTGCAGAAAGAACAGGAGGTGCTGGCAGCGCTGGGCATTGACACAACCTCGCTCTATTCCC ACAACGGATCACCCTTAGCCCCCAAAAACGCCAATGAGAGAAGACCCAGGGTGAAATCAAGGATGTCAGA AGGCTCGACAGATGACAACAGATCACTACAATCCGACCCTTACAACCAGCGCAGGATGTCTTTCCTAGGC CTTTCTTCTGGAAGACGCAGGGCTAGCCACAGCAGTGTGTTCCACTTCCGAGCACCCAGCCAAGACGTCT CATTTCCTGATGGGATCTTGGATGACGGGGTCTTTCATGGAGATCAGGAAAGCCGTCGAAGTTCCATATT GCTGGGCAGGGGTGCCGGGCAGGCAGGTCCTCTCCCCAGGAGTCCACTGCCTCAGTCCCCCAACCCTGGC CCTAGACGTGGAGAAGAGGGACAGCGTGGAGTGCCCACTGGTGAGCTTGCCACTGGAGCGCCTGAAGGCC CGGCACTCGATGCTGCAGGACAGAAGAACTTCCTGTCTGCAGACTACTTGAATGAACCTTTCCGAGCACA GAGGGCAATGAGTGTTGTCAGTATTATGACTTCTGTCATTGAGGAGCTGGAAGAGTCTAAGCTGAAGTGC CCACCCTGCTTGATCAGCTTAGCCCAGAAGTACCTGATATGGGAGTGCTGCCCCAAGTGGAAGAAATTCA AGATGGTGCTCTTCGAGCTGGTGACTGACCCCTTCGCAGAGCTCACCATCACCCTGTGCATTGTGGTGAA TACCGTCTTCATGGCCATGGAACACTACCCCATGACTGATGCCTTCGATGCCATGCTCCAAGCCGGCAAC ATTGTCTTTACTGTGTTTTTTACAATGGAGATGGCCTTCAAGATCATTGCCTTCGACCCCTACTACTACT TCCAGAAGAAGTGGAACATCTTCGACTGTGTCATCGTCACCGTGAGCCTGCTGGAGCTGAGCACATCCAA GAAGGGCAGCTTGTCTGTGCTCCGCACCTTCCGCTTGCTTCGGGTCTTCAAGCTGGCCAAGTCCTGGCCC ACCCTGAACATGCTCATCAAGATCATCGGGAACTCTGTGGGGGCCCTGGGCAACCTGACCTTCATCCTGG CCATCATCGTCTTTATCTTCGCCCTGGTGGGAAAGCAGCTCCTCTCAGAGAACTATGGGTGCCGCAGGGA TGGCATCTCCGTGTGGAATGGTGAGAGGCTGCGCTGGCACATGTGTGACTTCTTCCATTCCTTCCTCGTC GTCTTCCGGATCCTCTGCGGGGAGTGGATCGAGAACATGTGGGTCTGCATGGAGGTCAGCCAGGACTACA TCTGCCTCACCCTCTTCTTGACAGTGATGGTGCTAGGCAACCTGGTGGTGCTCAACCTATTCATCGCTTT ACTGCTGAACTCCTTCAGTGCGGACAACCTCACAGCCCCAGAGGATGACGGGGAGGTGAACAACTTGCAG GTAGCACTGGCCCGGATTCAGGTATTTGGCCATCGGGCCAGTCGGGCCATTACCAGTTACATCAGAAGCC ACTGCCGGCTCCGCTGGCCCAAGGTGGAGACCCAGCTGGGGATGAAACCCCCACTCACCAGCTGCAAAGC TGAGAACCACATTGCTACTGATGCTGTCAATGCTGCAGTGGGGAACCTGGCAAAGCCAGCTCTTGGTGGC CCCAAGGAGAACCACGGGGACTTCATCACTGATCCTAACGTGTGGGTCTCTGTGCCCATTGCTGAGGGGG AGTCCGACCTTGATGAGCTCGAGGAAGATGTGGAGCATGCTTCTCAGAGCTCCTGGCAGGAAGAGAGCCC CAAAGGGCAGCAGGAGCTGCTGCAGCAAGTCCAAAAGTGTGAAGATCACCAGGCAGCCCGAAGCCCACCC TCCGGGATGTCCTCTGAGGACCTGGCTCCATACCTGGGGGAGAGATGGCAGAGGGAGGAGAGCCCTCGGG TCCCTGCCGAGGGAGTGGATGACACAAGCTCCTCCGAGGGCAGCACGGTGGACTGCCCGGACCCAGAGGA GATCCTGAGGAAGATCCCTGAGCTGGCGGAGGAGCTGGACGAGCCCGATGACTGTTTCCCAGAAGGCTGC ACTCGCCGCTGTCCCTGCTGCAAAGTGAACACCAGTAAGTTTCCTTGGGCCACGGGCTGGCAGGTGCGCA AAACCTGTTACCGCATTGTGGAGCACAGCTGGTTTGAGAGTTTCATTATCTTCATGATCCTGCTCAGCAG TGGAGCGCTGGCCTTTGAGGATAACTACCTGGAAGAAAAGCCCCGAGTGAAGTCTGTGCTGGAGTACACT GACAGAGTGTTCACCTTCATCTTTGTATTCGAGATGTTGCTCAAGTGGGTAGCTTATGGCTTCAAAAAAT ATTTCACCAATGCCTGGTGCTGGCTGGACTTCCTCATCGTGAATATCTCCCTCACAAGCCTCATAGCCAA GATCCTCGAGTATTCAGACGTGGCGTCCATCAAAGCCCTTCGGACTCTCCGTGCCCTCCGGCCGCTGCGG GCTCTGTCTCGATTCGAAGGCATGAGGGTAGTGGTGGATGCCTTGGTGGGCGCCATTCCCTCCATCATGA ACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGGGTGTGAACCTCTTCGCCGGGAA ATTTTCGAGATGTGTCGACACCAGAAGCAACCCATTTTCCGTCGTGAATTCGACATTCGTGACTAACAAG TCTGACTGTTACAATCAAAACAATACTGGCCACTTCTTCTGGGTTAACGTCAAAGTCAACTTCGACAACG TTGCTATGGGCTACCTCGCGCTTCTCCAGGTGGCAACCTTCAAAGGCTGGATGGACATTATGTATGCAGC TGTCGATTCTCGAGATATCAACAGTCAGCCCAATTGGGAGGAGAGCCTGTACATGTACCTATACTTCGTC GTCTTCATCATTTTCGGTGGCTTCTTCACGCTGAATCTCTTTGTCGGGGTCATCATTGACAACTTCAATC AACAGAAAAAAAAGATAAGGGGCCAGGACATCTTCATGACAGAGGAGCAGAAGAAGTACTACAATGCCAT GAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCTTTGAATAAGTACCAGGGCTTCGTG TTTGACATTGTGACCAGGCAAGCATTTGACATCATCATCATGGCTCTCATCTGCCTCAACATGATCACCA TGATGGTGGAGACCGACAATCAGAGCGAGGAGAAGACGAAGGTCCTGGGCAGAATCAACCAGTTCTTCGT GGCCGTCTTCACGGGCGAGTGTGTGATGAAGATGTTCGCCCTTCGGCAGTATTACTTCACCAACGGCTGG AATGTGTTCGACTTCATTGTGGTGATTCTGTCCATTTCTAGTCTGTTGTTTTCTGCGATCCTTAGCTCAC TAGAAAGTTACTTCTCCCCCACGCTCTTACGCGTCATCCGTCTGGCCAGGATCGGCCGCATCCTCAGGCT GATTCGAGCAGCCAAGGGGATTCGCACGCTGCTCTTCGCCCTCATGATGTCCCTGCCCGCCCTCTTCAAC ATCGGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCCAGCTTCGCTAATGTCA TAGATGAGGCTGGCATCGACGACATGTTCAACTTCAAGACCTTTGGCAACAGCATGCTGTGCCTTTTCCA GATCACCACGTCGGCTGGCTGGGATGGCCTCCTCAGCCCCATCCTCAACACAGGACCCCCCTACTGCGAC CCCAACCGGCCCAACAGCAATGGCTCCAAGGGGAATTGTGGAAGCCCAGCGGTGGGCATCCTCTTCTTCA CCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATTGCAGTGATTCTGGAGAACTTCAA TGTGGCCACAGAAGAGAGCACGGAGCCCCTGAGCGAGGACGACTTTGACATGTTCTATGAGACCTGGGAG AAGTTTGACCCGGAGGCCACCCAGTTCATTGCCTTTTCTGCCCTCTCAGACTTTGCAGACACACTCTCTG GCCCTCTTAGAATCCCAAAACCTAATCAGAATATATTAATCCAGATGGACCTGCCGTTGGTCCCCGGAGA TAAGATCCACTGTTTGGACATCCTCTTTGCCTTCACAAAGAATGTCTTGGGAGAATCTGGGGAGTTGGAT TCTCTGAAGACTAATATGGAAGAGAAGTTTATGGCAACTAATCTTTCCAAAGCATCCTATGAACCAATAG CAACCACCCTCCGGTGCAAGCAGGAAGACATCTCAGCCACCATTATTCAAAAGGCCTATCGGAACTACAT GTTGCAACGCTCCTTGATGCTCTCCAACCCCCTGCATGTGCCCAGGGCTGAGGAGGATGGCGTGTCACTC CCCAGGGAAGGCTATGTTACATTCATGGCAAATGACAACGGTGGGCTCCCAGACAAATCGGAAACTGCTT CTGCTACGTCTTTCCCACCATCCTATGACAGCGTCACCAGGGGCCTGAGTGACAGGGCCAACATTAGCAC ATCTAGCTCAATGCAAAATGAAGATGAAGTCACTGCTAAGGAAGGGAAGAGCCCTGGACCTCAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001205321
Insert Size	5877 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001205321.1, NP_001192250.1
RefSeq Size	6416 bp
RefSeq ORF	5877 bp
Locus ID	20264
Gene Summary	Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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