Wnk3 (NM_001271679) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Prkwnk3; Wnk3-ps
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229655 representing NM_001271679 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTGATTCTGGGGAGCCAGCTAGCACAGAAGATTCTGAGAAGCCTGATGGAGTTTCCTTTGAAA ACAGGGCTGCCCGGGCTGTTGCCCCTCTGACTGTAGAAGCGAGAATAAAGGAAAAATACAGTACCTTTTC TGCTTCCGGGGAAAACATCGAAAGAAAGAGGTTTTTCCGAAAGAGTGTTGAGATGACAGAAGATGACAAA GTTGCCGAATCTTCCCGCAGAGATGAAAGAAAAGCTGCAACAAATATTTCAAGAGTAGATAAAGTTCCTA CAAATGTGCTAAGAGGTGGGCAAGAAGTTAAATATGAGCAATGTTCAAAGGCAACCTCAGAATCCTCAAA AGATTGCTTCAAGGAGAAAACTGAAAAGGAAATGGAAGAAGAAGCAGAAATGAAGGCTGTTGCAACTTCC CCTAGTGGCAGGTTCCTGAAATTCGATATAGAGCTGGGAAGAGGAGCATTTAAAACGGTATATAAAGGAT TGGACACTGAGACATGGGTTGAAGTTGCTTGGTGTGAGCTACAGGACCGCAAATTAACTAAAGCTGAGCA GCAACGGTTCAAAGAAGAAGCAGAGATGTTGAAAGGCCTCCAGCATCCCAATATAGTTCGATTCTATGAT TCATGGGAGTCCACTTTGAAAGGAAAGAAATGTATTGTGTTAGTGACTGAACTAATGACATCAGGAACCT TAAAGACGTACTTAAAACGATTTAAAGTCATGAAACCAAAGGTCTTAAGAAGCTGGTGCAGGCAAATCTT AAAAGGATTGCAGTTCTTACACACAAGGACGCCTCCTATTATTCACCGGGATCTGAAATGTGACAACATT TTCATCACAGGACCCACTGGATCTGTGAAGATTGGTGATCTGGGACTTGCAACCTTAATGCGCACATCGT TTGCTAAGAGTGTCATTGGCACTCCTGAATTTATGGCTCCGGAGATGTATGAAGAACACTATGATGAATC TGTAGATGTTTATGCTTTTGGAATGTGTATGTTGGAAATGGCTACTTCGGAGTATCCATATTCTGAGTGC CAGAATGCAGCACAAATATACCGGAAAGTAACCAGTGGCATAAAACCAGCCAGCTTCAATAAAGTCACAG ACCCTGAAGTGAAAGAAATAATTGAAGGATGTATTAGACAAAACAAATCTGAAAGGTTATCTATCAAGGA CTTACTAAACCATGCATTCTTTGCTGAGGATACTGGACTGAGGGTGGAGTTAGCAGAGGAAGATGATTGC TCAAATTCATCCTTGGCTCTAAGACTCTGGGTTGAAGATCCTAAGAAATTAAAAGGCAAACATAAAGACA ATGAAGCTATTGAATTCAGTTTCAACTTAGAAGCAGATACACCTGAGGAAGTAGCGTATGAAATGGTCAA GTCTGGATTCTTCCATGAAAGTGATTCCAAAGCTGTTGCTAAGTCCATTAGGGACCGAGTGACATTGATT AAAAAGATAAGGGAAAAAAAGCCTGCTGGATGCTTGGAGGAACGCAGGGATTCCCAGTGCAAGTATGTGA GGAATGTACTTCCTCAACAGCAGACTGCAACTTTACAACCTACTCCTGGTCCACACACTGCAGCTGAGTA TGAAGAAACAGAAGTTGATCAACATGTTCGACAGCAGTTTCTACAGGGCAAACCACAGCAGCAGTCCTCC TCTGTTAGGGGTGACACTTCATCTGAGCCAACAGCTGGACCAGTTCTACATTCAGATACTTCAAGTCATC CCACTGTAGCCTATTCTTCAAACCAGACAACTAGCTCTCAGGAGCAGCCAAAGCTGACTCAGTCCCCAGT TTTGCCTGTGGTTCAAGGTCAGTCCTCTGTTATGCCCATATATGCTGCTGGAGTTGGAGTTGTTTCACAG TCCCAAATCTCTCCCTTAACTATCCAGAAGGTCTCACAGATAAAGCCTGTATCCCAACCAATTGGAGCTG AACAACAAGCAACTCTTCAAAATCCAGATTTTGTTCGAAGCTTGAATCAAGATGTGACATCTGTGAAAGA AAACACCAATAACCCTGATACCCCAAGTGGAAATGGCAAACAGGATCGGAACAAACAACGAAGAGCCTCC TGTCCCCGACCAGAGAAGGGGACTAAATTCCAGCTTACTGTCCTTCAGGTCTCAGTCTCTGGAGACAACA TGGTGGAGTGTCAGCTGGAAACACATAACAACAAGATGGTGACATTCAAGTTTGATGTTGATGGTGACGC ACCTGAGGATATCGCTGACTATATGGTTGAAGATAACTTTGTGCTGGAAAATGAGAAAGAAAAATTTGTC GAGGAATTGAGGGCTATCGTAGGTCAAGCCCAGGAGATCCTTCATGTCCACTCAGCTGTAGAAAAATCTA TTGGTGTTGACTCTGTTGCCTTGGAATCTAACAGTAACCAAACAGGATCATCTGAACAAGTACTGATAAA TTCTGCATCCACTCAAACTAGCAATGAATCTGCTCCTCAGTCATCCCCAGTTGGTCGCTGGCGGTTTTGT ATCAACCAAACAATAAAAAACCGTGAGGCACAGTCTCCTCCTTCCCTTCAGCCTTCCATGGCCATGGTGC CTGGGTTACATCCATTTCCTAGTTCAAGAAACACAAGTAATCAGGCAATATCACAGAACACAGTGTTCAC TATAGAGAATAATCCAGGCCATCGTGAACTTTTCACCTCCAAATTAGACCACAAGGATGTGGTTGATGGT AAGATTGGTGAACATGCTAGTATAGAAACTGAGCAGTCAAGTATTTCCTATCAAGTGGAAGATGACAGAC AGATCATGACACCAGCTACTGATAATTCCAATTATTCTGCTGCTTTGGTGTGTCCAGTTCCAGGTGAATG TGAGGCACTCACCAGCCAAGCAGGCATGTTCATGCCTACATATCCAAATCAGCAAGCTGCTGTTCTGGCT GATGTGCATATAGCCTATCCCGGTGAATCAGTTCCAATTGGTGGTAATGCTGCTCTTACATCAGTACTTG TATCTTCTGATCAAAAGCCTCAATCCTTGTCAGTACAGCAGCCAACTATAGATGCAGAGTTTATTTCCAA AGAAGGAGAAACCACAGTAAACACCGAAACAAGTTCTCCTAAGGCAGTCATTGCCACTCAGACTCCTGGC TTTGAACCAGCTGTGATTCTACCTGCTACTATCCTGGAATCAGATGGGGAACGACCTCCAAAAATGGAGT TTGCAGACAACCGAATTAAAACACTGGATGAAAAATTAAGAAACCTGCTCTATCAGGAGCACAGCATTTC TAGCATCTGTCCTGAGAGTCAGAAGGATACTCAAAGCATAGACTCTCCATTTTCTTCCTCTGCTGAAGAT ATACTATCCTATTCAATGCCAGAAGTCATAGCCATCAGTCACTGTGGGATTCAAGATAGCCCTGCACAAT CCCCAAATTTCCAACAAACAGGCTCTAAGATTCTGTCCAATGTGGCTGCAAGTCAGCCTGCTCATATATC AGTGTTCAAAAAAGACCTGAATGTGATAACTTCTGTACCCAGCGAGTTATGTTTACATGAGATGTCCCCA GATGCTTCACTTCCAGGCGATCCAGAGGCCTATCCTGCTGCTGTGTCAAGCGATGGAACCATTCATCTGC AGACAGGAGTGGAAACAGAAGATAAAAGATCAGCAATTGCTTCTGATCCCATTCCTCTGACACGGGAGTT CTCATCTGATACTAGGGCTTTGAGTAGATGTAAAGCGATGAGTGGATCATTTCAGCGGGGTCGGTTCCAG GTGATTACTGTTCCTCAGCAGCAGCCAGTAAAAATGATGTCTTTTGGGAAAGATCACAGACCGCCATTCA ACAAAACAACAGTCCAGTCTAGTGAGCAAGCACTAACCTTTGCTGAAGCTGCAGTGTCGCAGTTGATCGA AGTGGAGCCTGCCATGCCCACTCATAAAGCTTCAGTTTCTTCACGCAAGTTGCGAACTCTTTATGAAACC TTTAAAGAAGATAAAGGAGATCCTGAACAAGGTGACATCGTATCTTACAGCACAGCTTGTGAGACCAGTG TCTCTTCAGTCGCTACAGAAAAGAATGTAACTTCAACCACAGAAGTTAGTGTGCAGTCTGGGTCTGAACC GTTAGATAAAGAAAAAAATGAGTCAACTCCTGGGAAACAGACATGTACAAATGAATTTTCAGCCACTCTT GCTGGTAATAGAAAGTCCGTGACAAAAACTCGTCCAGAGGGTGATCAGTATTTACCACTCCGTGAAGAGC AAGCCTATGCTCAAACACAGAATTCACTCTTCTATTCTCCATCTTCCCCAATGAGCAGTGACAATGAATC AGAAATTGAGGACGAGGACTTGAAAGTGGAGCTTCAAAGATTACGAGAAAAACACATTCAAGAGGTGGTC AGTCTTCAAACACAACAGAATAAAGAGCTACAAGAGCTCTATGAACGCCTTCGAGCAACTAAAGACAACA AAGCGCAATCTTCAGAGGTTCCTTTGTCACCTGCATCGCCACGCAGGCCAAGATCTTTCAAAAGCAAACT TCGAAGCCGCCCCCAGTCCATGACACATTCAGACAATCTCGTTGTAAAAGATGCACTGGGTGTGGAGAGT AATACCGTGTCATGCCAGCAATCTCCAGCCAGTAAGAAGGGGATGTTCACAGATGATTTACACAAGCTGG TGGATGACTGGACAAGAGAAACAGTAGGGCATTTTCCTAGTAAGCCAAGTTTAAACCAACTCAAACAGAG CCAACAAAAATCCGAAGCAGAAAACTGGAACAAGTCATGTGAAAGTACTCCATCTACTATGGGATACACG TCAAACTGGATTTCTTCTCTCTCCCAAATTCGTGGAGCTGCCCCAACTTCCTTGCCACAAGGACTCCCAC TCCCTTCATTTCATGGGCCATTAGCATCATATGGAATGCCTCACGTTTGTCAGTATAATGCTGTGGGTGC CGCAGGTTATCCAGTACAGTGGGTAGGAATTTCTGGACCAGCACAACAGTCTGTAGTGCTTCCTACCCAA TCTGGGGGACTCTTCCAGCCCGGGATGAATTTGCAGTCATTTCCAGCTCCACCAGTGCAGAATCCAGCTT CAATCCCTCCTGGTCCTAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271679
Insert Size	5133 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271679.1, NP_001258608.1
RefSeq Size	10259 bp
RefSeq ORF	5133 bp
Locus ID	279561
UniProt ID	Q80XP9
Gene Summary	Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A1, SLC12A2, SLC12A3, SCNN1A, SCNN1B, SCNN1D and SGK1. Inhibits SLC12A4, SLC12A5, SLC12A6 and SLC12A7. Phosphorylates WNK4. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternated splice site in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.

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