Itsn2 (NM_001198968) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI327390; Ese2; mKIAA1256; Sh3d1B; Sh3p18
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229649 representing NM_001198968 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCTCAGTTTCCCACAGCGATGAATGGAGGGCCAAATATGTGGGCTATTACATCTGAAGAACGTA CTAAGCATGATAAACAGTTTGATAACCTCAAACCTTCAGGAGGTTACATAACAGGTGATCAAGCCCGTAC TTTTTTCCTACAGTCAGGTCTGCCGGCCCCGGTTTTAGCTGAAATATGGGCCTTATCAGATCTGAACAAG GATGGGAAGATGGACCAGCAAGAGTTCTCTATAGCTATGAAACTCATCAAGTTAAAGTTGCAGGGCCAAC AGCTGCCTGTAGTCCTCCCTCCTATCATGAAACAACCCCCTATGTTCTCTCCACTAATCTCTGCTCGTTT TGGGATGGGAAGCATGCCCAATCTGTCCATTCATCAGCCATTGCCTCCAGTTGCACCTATAGCAACACCC TTGTCTTCTGCTACTTCAGGGACCAGTATTCCTCCCCTAATGATGCCTGCTCCCCTAGTGCCTTCTGTTA GTACATCCTCATTACCAAATGGAACTGCCAGTCTCATTCAGCCTTTATCCATTCCTTATTCTTCTTCAAC ATTGCCTCATGCATCATCTTACAGCCTGATGATGGGAGGATTTGGTGGTGCTAGTATCCAGAAGGCCCAG TCTCTGATTGATTTAGGATCTAGTAGCTCAACTTCCTCAACTGCTTCCCTCTCAGGGAACTCACCTAAGA CAGGGACCTCAGAGTGGGCAGTTCCTCAGCCTTCAAGATTAAAGTATCGGCAAAAATTTAATAGTCTAGA CAAAGGCATGAGCGGATACCTCTCAGGTTTTCAAGCTAGAAATGCCCTTCTTCAGTCAAATCTCTCTCAA ACTCAGCTAGCTACTATTTGGACTCTGGCTGACATCGATGGTGACGGACAGTTGAAAGCTGAAGAATTTA TTCTGGCGATGCACCTCACTGACATGGCCAAAGCTGGACAGCCACTACCACTGACGTTGCCTCCCGAGCT TGTCCCTCCATCTTTCAGAGGGGGAAAGCAAGTTGATTCTGTTAATGGAACTCTGCCTTCATATCAGAAA ACACAAGAAGAAGAGCCTCAGAAGAAACTGCCAGTTACTTTTGAGGACAAACGGAAAGCCAACTATGAAC GAGGAAACATGGAGCTGGAGAAGCGACGCCAAGTGTTGATGGAGCAGCAGCAGAGGGAGGCTGAACGCAA AGCCCAGAAAGAGAAGGAAGAGTGGGAGCGGAAACAGAGAGAACTGCAAGAGCAAGAATGGAAGAAGCAG CTGGAGTTGGAGAAACGCTTGGAGAAACAGAGAGAGCTGGAGAGACAGCGGGAGGAAGAGAGGAGAAAGG AGATAGAAAGACGAGAGGCAGCAAAACAGGAGCTTGAGAGACAACGCCGTTTAGAATGGGAAAGACTCCG TCGGCAGGAGCTGCTCAGTCAGAAGACCAGGGAACAAGAAGACATTGTCAGGCTGAGCTCCAGAAAGAAA AGTCTCCACCTGGAACTGGAAGCAGTGAATGGAAAACATCAGCAGATCTCAGGCAGACTACAAGATGTCC AAATCAGAAAGCAAACACAAAAGACTGAGCTAGAAGTTTTGGATAAACAGTGTGACCTGGAAATTATGGA AATCAAACAACTTCAACAAGAGCTTAAGGAATATCAAAATAAGCTTATCTATCTGGTCCCTGAGAAGCAG CTATTAAACGAAAGAATTAAAAACATGCAGCTCAGTAACACACCTGATTCAGGGATCAGTTTACTTCATA AAAAGTCATCAGAAAAGGAAGAATTATGCCAAAGACTTAAAGAACAATTAGATGCTCTTGAAAAAGAAAC TGCATCTAAGCTCTCAGAAATGGATTCATTTAACAATCAGCTGAAGTGTGGGAGTATGGATGACTGTGTC CTTCAGTGCCTTTTGTCTCTGCTGAGCTGTCTCAACAACCTCTTCCTCTTACTTAAGGAACTCAGAGAAA GCTATAATACACAGCAGTTAGCCCTTGAACAACTTCATAAAATCAAACGTGACAAATTGAAGGAAATCGA AAGAAAAAGATTAGAGCAAATTCAAAAAAAGAAACTAGAAGATGAGGCTGCAAGGAAAGCAAAGCAAGGA AAAGAAAACTTGTGGAGAGAAAGTATTAGAAAGGAAGAAGAGGAAAAGCAAAAACGACTCCAGGAAGAAA AGTCACAGGACAAAACTCAAGAAGAGGAACGAAAAGCTGAGGCAAAACAAAGTGAGACAGCCAGTGCTTT GGTGAATTACAGAGCACTGTACCCTTTTGAAGCAAGAAACCATGATGAGATGAGTTTTAGTTCTGGGGAT ATAATTCAGGTTGATGAAAAAACTGTAGGAGAGCCTGGTTGGCTTTATGGTAGTTTTCAGGGAAAGTTTG GCTGGTTCCCGTGCAACTATGTAGAAAAAGTGCTGTCAAGTGAAAAAGCTCTGTCTCCTAAGAAGGCCTT ACTTCCTCCTACAGTGTCTCTCTCTGCTACCTCAACTTCTTCCCAGCCACCAGCATCAGTGACTGATTAT CACAATGTATCCTTCTCAAACCTTACTGTTAATACAACATGGCAGCAGAAGTCAGCTTTTACCCGCACTG TGTCCCCTGGATCTGTGTCCCCCATTCACGGACAGGGGCAGGCTGTAGAAAACCTGAAAGCCCAGGCCCT TTGTTCCTGGACGGCAAAGAAGGAGAACCACCTGAACTTCTCAAAGCACGACGTCATCACTGTCCTGGAG CAGCAGGAAAACTGGTGGTTTGGGGAGGTGCACGGAGGAAGAGGATGGTTCCCCAAGTCTTATGTCAAGC TCATTCCTGGGAATGAAGTACAGCGAGGAGAGCCAGAAGCTTTGTATGCAGCTGTGACTAAGAAACCTAC CTCCACAGCCTATCCAGTTACCTCCACAGCCTATCCAGTTGGAGAAGACTACATTGCACTTTATTCATAC TCAAGTGTAGAGCCCGGGGATTTGACTTTCACTGAAGGTGAAGAAATTCTAGTGACCCAGAAAGATGGAG AGTGGTGGACAGGAAGTATTGGAGAGAGAACTGGAATCTTCCCGTCCAACTACGTCAGACCAAAGGATCA AGAGAATTTTGGGAATGCTAGCAAATCTGGAGCATCAAACAAAAAACCCGAGATCGCTCAAGTAACTTCA GCATATGCTGCTTCAGGGACTGAGCAGCTCAGCCTTGCGCCAGGACAGTTAATATTAATCTTAAAGAAAA ACACAAGCGGGTGGTGGCAAGGAGAGCTACAGGCCAGAGGGAAGAAACGACAGAAGGGATGGTTTCCTGC CAGCCATGTAAAGCTGCTAGGTCCAAGCAGTGAAAGAACCATGCCTACTTTTCACGCTGTATGTCAAGTG ATTGCTATGTATGACTACATGGCGAATAACGAAGATGAGCTCAATTTCTCCAAAGGACAGCTGATTAATG TTATGAACAAAGATGACCCTGACTGGTGGCAAGGAGAAACCAATGGTCTGACTGGTCTCTTTCCTTCAAA CTATGTTAAGATGACAACAGACTCAGATCCAAGTCAACAGTGGTGTGCTGACCTCCAAGCCCTGGACACA ATGCAGCCTACGGAGAGGAAGCGACAGGGCTACATTCACGAGCTCATTCAGACAGAGGAGCGGTACATGG ACGACCTGCAGCTGGTCATCGAGGTCTTCCAGAAACGGATGGCTGAGTCAGGCTTCCTCACTGAAGCAGA CATGGCTCTGATCTTTGTGAACTGGAAAGAGCTCATCATGTCCAACACGAAGCTGCTGAGGGCCTTGCGG GTGAGGAAGAAGACTGGGGGTGAGAAGATGCCAGTTCAGATGATTGGAGACATCCTGGCGGCAGAGCTGT CCCACATGCAGGCCTACATCCGCTTCTGCAGCTGTCAGCTTAATGGGGCAACCCTGTTACAGCAGAAGAC AGACGAGGACACGGACTTCAAGGAATTTCTAAAGAAGTTGGCATCAGACCCACGATGCAAAGGGATGCCC CTCTCCAGCTTCCTGCTGAAGCCCATGCAGAGGATCACTCGCTACCCGCTGCTCATCCGAAGTATCCTGG AGAACACTCCACAGAGTCATGTTGACCACTCCTCCCTGAAGCTGGCCCTAGAACGTGCTGAGGAGCTGTG CTCTCAGGTGAACGAGGGAGTCCGGGAGAAGGAAAATTCAGACCGGCTGGAGTGGATCCAGGCACACGTG CAGTGCGAAGGCTTGGCAGAGCAACTTATTTTCAACTCCCTCACCAACTGCCTGGGCCCCCGGAAGCTTC TGCACAGCGGGAAGCTGTACAAGACCAAGAGCAATAAGGAGCTGCACGCCTTCCTCTTCAACGACTTCCT GCTGCTCACCTACCTGGTCAGGCAGTTTGCCGCCGCCTCTGGCCACGAGAAGCTCTTCAACTCCAAGTCC AGTGCTCAGTTCCGGATGTACAAAACGCCCATTTTCCTGAATGAAGTGTTGGTGAAACTTCCCACAGACC CTTCCAGCGATGAGCCCGTCTTCCACATTTCCCACATTGATCGTGTGTACACACTCCGAACAGACAACAT CAACGAGAGGACGGCCTGGGTCCAGAAGATCAAGGGTGCCTCAGAGCAGTACATCGACACTGAGAAGAAG AAACGGGAAAAGGCTTACCAAGCCCGTTCTCAAAAGACTTCAGGTATTGGGCGTCTGATGGTGCATGTCA TTGAAGCTACAGAATTAAAAGCCTGCAAACCAAACGGGAAAAGTAATCCATACTGTGAAGTCAGCATGGG CTCCCAAAGCTATACCACCAGGACCCTGCAGGACACACTAAACCCCAAGTGGAACTTCAACTGCCAGTTC TTCATCAAGGATCTTTACCAGGACGTTCTGTGTCTCACTATGTTTGACAGAGACCAGTTTTCTCCAGATG ACTTCTTGGGTCGTACTGAAGTTCCAGTGGCAAAAATCCGAACAGAACAGGAAAGCAAAGGCCCCACCAC CCGCCGACTACTACTGCACGAAGTCCCCACTGGAGAAGTCTGGGTCCGCTTTGACCTGCAACTTTTTGAA CAAAAAACTCTCCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198968
Insert Size	5058 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198968.2, NP_001185897.1
RefSeq Size	6082 bp
RefSeq ORF	5058 bp
Locus ID	20403

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