Clasp1 (NM_001293300) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700030C23Rik; 5730583A19Rik; B130045P17Rik; CLASP1alpha
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229628 representing NM_001293300 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCGAGAATGGAGTCCTGTCTGGCCCAGGTGCTGCAGAAGGATGTGGGAAAGCGGCTGCAGGTTG GCCAGGAACTCATAGACTATTTCTCAGACAGACAGAAGTCTGCTGACCTTGAGCACGACCAGACCCTGTT GGATAAGCTTGTGGATGGACTCGCTACCTCTTGGGTGAACTCTAGCAATTACAAGGTGGTTCTCTTGGGC ATGGACATCCTGTCGGCACTGGTGACTAGGCTGCAGGACCGGTTCAAGGCGCAAATCGGCACAGTGTTGC CAAGTCTAATAGACAGACTGGGAGATGCTAAAGACTCCGTGAGGGAGCAAGACCAAACCCTGCTGCTAAA GATCATGGATCAAGCTGCTAATCCCCAGTATGTGTGGGACAGAATGCTCGGAGGCTTCAAACACAAGAAC TTCCGCACAAGAGAGGGCATCTGCCTCTGCCTTATTGCAACACTCAATGCCTCTGGGGCCCAGACTCTAA CACTAAGCAAGATTGTGCCACATATATGTAACCTACTGGGAGATCCCAACAGCCAGGTTCGAGACGCAGC AATAAACAGTCTGGTGGAGATTTATAGACATGTAGGTGAACGTGTGAGGGCAGACCTCAGTAAGAAAGGA CTGCCACAGTCCCGGTTGAATGTCATTTTTACAAAATTTGATGAAGTCCAAAAGTCTGGAAATATGATAC AGTCTGCAAATGAAAAAAATTTTGATGATGAAGATTCTGTGGATGGCAACAGGCCTTCTTCTGCCAGCTC CTCATCATCCAAGGCCCCATCAAGTTCCCGGAGGAATGTTAACCTGGGGACCACCCGTAGGCTCATGTCA TCCAGTCTTGGATCTAAGTCTTCAGCTGCAAAAGAAGGCGCTGGTGCTGTGGATGAAGAGGATTTTATTA AAGCCTTTGATGATGTACCTGTAGTGCAGATTTACTCCAGCCGAGACCTCGAGGAATCCATAAACAAAAT CAGAGAAATCCTGTCAGATGACAAGCATGACTGGGAGCAGAGAGTAAATGCTCTAAAAAAGATTAGATCG TTACTCTTGGCTGGGGCTGCTGAGTACGATAACTTCTTTCAACACTTGCGTCTTCTGGACGGGGCCTTTA AACTCTCTGCTAAGGACCTGCGGTCTCAGGTAGTGCGGGAAGCTTGTATCACATTGGGGCATCTGTCATC AGTTCTGGGAAATAAGTTTGATCATGGAGCTGAAGCCATTATGCCAACTATCTTTAATTTAATCCCGAAC AGTGCCAAAATTATGGCTACTTCTGGTGTTGTAGCTGTTAGGCTAATCATTCGGCACACTCACATCCCTC GGCTGATCCCTGTCATAACCAGCAACTGTACCTCTAAGTCTGTCGCCGTCAGAAGGCGCTGTTTTGAATT TTTAGATTTGCTTTTACAAGAATGGCAGACACATTCACTAGAAAGACATATATCAGTATTAGCTGAAACA ATAAAGAAAGGAATACACGACGCTGATTCTGAAGCGAGAATAGAAGCCAGGAAGTGCTACTGGGGTTTCC ACAGTCACTTCAGCCGAGAAGCAGAACACCTGTACCACACTTTGGAGTCCTCGTATCAGAAGGCCCTACA GTCCCACTTGAAGAACTCGGACAGCATCGTGTCTCTGCCCCAGTCAGACCGATCCTCTTCCAGCTCTCAA GAGAGTCTCAACCGGCCACTTTCAGCCAAAAGAAGTCCCACTGGCAGCACTGCATCCAGAGGCTCTACAG TTAGTACCAAATCTGTGTCGACGACAGGATCTCTCCAGCGATCTCGAAGTGATATTGATGTGAACGCAGC AGCCAGTGCCAAATCCAAAGTCTCCTCATCCTCAGGATCCCCCGCCTTCAGCTCTGCAGCAGCACTGCCC CCAGGATCCTATGCGTCCCTAGACGGTACTACCACTAAAGCGGAGGGTCGGATCCGCACGAGACGGCAGA GCTCGGGGAGCACCACCAATGTCGCCTCCACACCCTCCGACAGTCGGGGCCGCAGTCGCGCCAAAGTGGT TTCACAGTCTCAGCGATCCAGATCTGCTAACCCTGCTGGTGCTGGCAGCCGGTCAAGTTCCCCTGGGAAG CTTTTGGGAAGTGGACTTGCTGGGGGTTCCTCCAGAGGCCCACCGGTAACACCATCCTCAGAAAAACGGA GCAAGATCCCCAGGAGTCAGGGATGTAGCCGAGAAACAAGTCCTAACCGGATTGGATTAGCACGGAGCAG CCGTATCCCCCGACCCAGCATGAGTCAGGGGTGCAGCCGCGATACCAGCCGTGAGAGCAGCCGCGATACA AGCCCTGCTCGGGGCTTCACACCACTCGATCGGTTTGGGCTGGGCCAGTCAGGACGAATCCCTGGTTCTG TGAACGCCATGAGAGTCTTGAGTACCAGCACTGACCTGGAAGCAGCAGTGGCTGACGCTCTGCTGTTAGG AGACGCCAGGAGCAAGAAGAAGCCTGTGAGAAGGAGATACGAGCCCTATGGAATGTACTCTGATGATGAT GCCAACAGTGATGCCTCCAGCGTGTGCTCTGAGCGCTCATATGGCTCCAGGAATGGTGGCATTCCCCATT ATCTGCGGCAGACTGAAGATGTAGCAGAGGTTCTCAACCATTGTGCCAGTTCCAACTGGTCAGAACGGAA AGAGGGGCTCCTGGGCCTGCAGAACTTACTGAAGAGCCAAAGAACACTGAGTCGAGTAGAATTGAAGAGA CTGTGTGAGATTTTCACCCGAATGTTTGCCGACCCTCACAGCAAGAGAGTTTTCAGTATGTTTTTGGAGA CCCTCGTGGATTTTATAATCATTCATAAGGATGACTTGCAAGACTGGCTCTTCGTCCTTCTCACACAACT ACTTAAGAAAATGGGAGCAGACTTACTTGGATCTGTGCAAGCAAAAGTTCAGAAGGCTCTCGATGTCACC AGGGATTCCTTTCCATTTGATCAACAATTTAACATTTTGATGAGATTTATTGTGGATCAGACTCAAACTC CAAACCTCAAGGTCAAAGTAGCAATCCTGAAATACATCGAATCTCTAGCCAGACAGATGGACCCCACAGA TTTTGTAAATTCCAGCGAAACAAGACTAGCTGTTTCTAGGATCATAACATGGACTACAGAACCAAAGAGT TCAGACGTGAGAAAGGCAGCACAAATTGTGCTCATCTCTCTGTTTGAACTGAACACGCCTGAATTTACCA TGTTACTTGGTGCCTTGCCAAAAACATTCCAGGATGGTGCCACCAAACTCCTGCATAACCACCTCAAGAA CTCCAGTAACACCGGTGTGGGATCTCCAAGCAATACAATTGGCCGGACACCTTCCCGCCACCCCAGCAGC AGGACCAGCCCCCTGACCTCACCCACCAACTGTTCCCATGGGGGACTATCTCCAAGTCGGTTGTGGGGTT GGAGTGCCGACGGGCTATCGAAGCCCCCACCTCCCTTTTCTCAGCCTAACTCCATTCCCACCGCTCCCTC CCACAAGACTCTCAGGCGCTCTTACTCTCCCAGCATGCTGGACTATGATACAGAGAACCTGAACTCTGAA GAAATCTACAGCTCTTTGCGTGGAGTTACAGAAGCCATTGAAAAGTTCAGCTTCCGAAGCCAGGAGGATC TAAATGAGCCAATCAAACGAGATGGCAAGAAGGATTGTGATATCGTGTCCCGAGATGGGGGAGCAGCCTC ACCTGCCACCGAGGGCCGGGGAGGTAGTGAGATAGAAGGAGGCAGGATGGCTTTGGACAACAAGACCTCC CTGCTCAACACGCAGCCTCCACGTGCCTTTCCGGGGCCAAGAGCACGGGAATATAACCCGTATCCCTACT CCGACACCATCAACACCTATGACAAGACGGCTCTGAAGGAAGCAGTGTTTGACGATGACATGGAGCAGCT CCGAGATGTGCCCATTGACCACTCAGACCTGGTGGCTGACTTGCTGAAAGAGCTATCTAACCACAACGAG CGTGTGGAGGAGCGGAAGGGCGCACTGCTGGAGTTGCTCAAGATCACCAGGGAGGACAGCCTGGGCGTGT GGGAGGAGCACTTCAAGACCATCCTGCTGCTGCTGCTGGAAACTCTCGGAGACAAAGACCATTCCATTCG AGCTCTGGCACTGAGAGTTTTACGGGAAATTCTGAGAAACCAGCCAGCAAGATTCAAAAACTATGCAGAA CTGACGATCATGAAGACTCTGGAAGCCCACAAAGACTCCCACAAGGAGGTGGTGAGAGCGGCCGAGGAAG CTGCATCCACGCTAGCCAGCTCAATCCACCCAGAGCAGTGCATCAAAGTGCTGTGTCCAATCATCCAGAC AGCCGACTACCCCATCAACCTGGCTGCTATCAAGATGCAGACAAAGGTGGTGGAGAGGATCACCAAGGAG TCCTTGCTGCAGCTCCTCGTCGACATCATCCCCGGCCTGCTGCAGGGTTACGACAACACCGAGAGCAGTG TACGGAAAGCCAGTGTGTTTTGCTTAGTGGCAATCTATTCCGTAATCGGAGAAGATCTGAAACCTCACCT CGCACAGCTCACGGGGAGCAAGATGAAGCTGCTGAACTTATATATAAAGAGGGCCCAGACTACCAACAGC AACAGCAGCTCTTCCTCTGATGTGTCCACACACAGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293300
Insert Size	4659 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293300.1, NP_001280229.1
RefSeq Size	7951 bp
RefSeq ORF	4659 bp
Locus ID	76707
UniProt ID	Q80TV8
Gene Summary	Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) encodes isoform (4). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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