Rapgef6 (NM_001252496) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A530068K01; AI844632; BB085664; C030018K18Rik; mKIAA4052; PDZ-GEF2; Pdzgef2; RA-GEF-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229615 representing NM_001252496 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCGCCCGTGGACCCCGGCGCTCGGCAGGCACTGAGGAAGAAGCCGCCCGAGCGGACTCCTGAGG ACTTAAATATTATTTATTCTTATCTGCATGGGATGGAAATACTATCAAACCTCAGGGAGCATCAGCTTAG GTTAATGTCTACAAGAGCACGCTATGAGAGATACAGTGGCAATCAAATGCTTTTTTGTTCAGAAACTATT GCCAGATGTTGGTATATCCTGCTTTCTGGATCTGTACTTGTAAAAGATTCTATGGTCCTTCCTCCTTGCA GTTTTGGTAAGCAGTTTGGAGGAAAAAGAGGATGTGATTGTCTTGTGTTAGAACCTTCAGAAATGATTGT GGTAGAGAATTCCAAAGATAATGAAGACAGTATTTTACAAAGAGAAATTCCTGCCAGACAGTCTCGACGA AGGTTTCGGAAAATTAACTATAAAGGAGAACGCCAAACCATAATAGATGGAGTGGATATTAATAACTACC TTTCGCTTCCAGCTGATCTTACCAAGATGCACCTCACAGACAACCCTCATCCACAGGTGACGCATGTGTC TTCTAGTCAGTCTGGCTGTAGCATTGCCAGTGACTCTGGGAGCAGCAGTTTATCCGATATCTATCAGGCT ACAGAGAGTGAAGTAGGAGATGTAGATTTAACACGCCTTCCAGAAGGACCTGTTGATTCTGAGGATGAAG AGGAGGAGGAAGAAGAGATTGATAGAACAGATCCACTGCAGGGTCGAGATCTTGTTCGGGAGTGTCTTGA AAAGGAACCCGCAGACAAAACTGATGATGATGTTGAACAGTTACTTGAGTTCATGCACCAGCTGCCTGCC TTTGCGAACATGACCATGTCTGTAAGGAGAGAACTCTGCTCCGTCATGGTTTTTGAAGTAGTAGAGCAGG CTGGGGCTGTTATTCTTGAAGATGGGCAAGAGCTTGACTCATGGTATGTCATTTTAAATGGCACTGTAGA AATTAGTCATCCAGATGGGAAAATAGAAAATCTCTTCATGGGAAATAGTTTTGGGATTGTCCCCACGCTG GATAAGCAGCACATGCATGGAGCTGTCAGGACCAAAGTCGATGATTGTCAGTTTGTCTGCATAGCTCAAC AAGACTATTGGAGAATTTTAAATCATGTGGAAAAAAATACTCACAAAGTTGAGGAAGAGGGAGAGATTGT CATGGTACATGAGCACCGTGAGCTAGACCGGAGCGGAACCAGGAAAGGACACATTGTAATCAAGGCAACA CCTGAGCGTCTCATCATGCATTTGATAGAAGAACATTCTATTGTGGACCCAACATACATAGAAGATTTCC TCTTAACTTACAGGACATTTCTTGAAACTCCTTTGGATGTTGGGATCAAGCTTTTGGAATGGTTTAAGAT TGACAACCTAAGGGATAAGGTGACACGGATTGTACTCTTATGGGTAAATAATCATTTTAATGATTTTGAA GGTGATCCTGCTATGACTCAATTTCTAGAAGAATTTGAAAGAAATTTGGAAGATACAAAAATGAATGGTC ACCTCAGGTTATTGAATATTGCCTGTGCTGCAAAGGCTAAGTGGAGGCAAGTTGTACTGCAGAAGGCGTC CCGTGAGTCCCCGCTGCACTTCTGCCTTACTGGGGGCAGTGAGAAGGGATTTGGAGTCTTTGTTGAAGAA GTAGAATCTGGTAGCAAAGCTGCTGACGCAGGACTGAAAAGGGGTGATCAGGTAATGGAAGTCAATGGAC AAAACTTTGAGAATATTACACTTGCTAAAGCCCTTGAAATTTTGAGAAATAATACTCATCTTGCACTTAC TGTGAAGACCAACATTTTTGTGTTCAAAGAATTGCTCAGTAGGACTGAACAAGAGAAATCTGGTGTTCCT CATATTCCCAAAATTGCTGAAAAAAAAAGTAATCGCCATTCAATTCAAGATGTCCCAGGGGATATGGAGC AGGCACCACAGGAGAAAGGAAATAAGAAAATTAAAGCAAATACCGTTTCAGGTGGAAGAAACAAAATCAG GAAAATTTTGGATAAAACACGATTTAGTATCTTGCCTCCAAAACTATTTAGTGACGGAGGCCTGAGTCAA TCACAAGATGACAGCATTGTGGGAACCAGGCACTGTAGGCACAGCCTGGCTATAATGCCAATTCCCGGAA CACTTTCATCCAGCAGCCCAGATCTCTTACAGCCTACCACTAGCATGTTGGACTTTTCCAATCCTTCAGC TGTTGGTTTTTACTATATTCCTGATCAAGTTATAAGAGTTTTCAAAGCAGATCAACAAAGTTGCTACATT ATCATCAGTAAGGATACCACAGCTAAAGAAGTGGTCTGCCAAGCTGTACAGGAATTCGGTTTGACTGGTG CATCTGACACATACTCCCTCTGTGAAGTGTCTGTTACTCCTGAGGGTGTTATAAAGCAGAGAAGACTTCC GGACCAGTTCTCCAAATTAGCTGATAGAATTCAACTCAATGGAAGGTATTACCTAAAAAACAACATGGAA ACAGAAACATTGTGTTCTGATGAAGATGCTCAGGAACTACTTAAGGAAAGCCAGCTCTCCATGCTGCAGC TCAGTACCATCGAGGTCGCCACCCAGCTCTCCATGAGAGACTTTGATTTATTTCGTAACATTGAGCCTAC TGAATACATTGATGATCTTTTTAAATTAGATTCCAAAACAGGAAATACTCACTTGAAACAATTTGAGGAT ATTGTAAACCAAGAGACATTCTGGGTTGCCTCAGAGATTTTAAGTGAGTCAAATCAGCTGAAAAGAATGA AGATTATTAAACATTTTATCAAAATTGCTCTTCATTGCCGAGAATGCAAGAATTTCAATTCCATGTTTGC AATAATAAGTGGCTTGAACCTGGCACCGGTAGCACGACTCAGAGGCACTTGGGAAAAGTTGCCAAGCAAA TATGAGAAACATCTTCAAGATCTACAAGACCTTTTTGATCCATCTAGAAATATGGCAAAATACAGAAATA TTCTTAGTAGTCAAAGCATGCAGCCTCCAATTATTCCACTCTTCCCTGTTGTCAAGAAAGATATGACATT TCTCCATGAAGGAAATGACTCCAAAGTAGATGGCTTAGTAAACTTCGAGAAGCTAAGAATGATTGCCAAG GAAATCCGTCATATTATTCGAATGACTTCTGCCAACATGGACCCTGCCATGATGTTCCGACAGAGGAAGA AGAGGTGGCGGAGTCTGGGGTCATTGAGTCAAGGCAGCACAAATTCTAACATGCTAGATGTCCAGGGAGG TGCTCACAAAAAAAGAGCACGCCGCAGCTCACTGCTGAATGCCAAGAAACTGTATGAAGATGCCCAAATG GCAAGGAAAGTGAAACAGTATCTTTCTAGTCTGGACATAGACACAGATGAGGAGAAGTTCCAGATGATGT CACTGCAGTGGGAACCTGCATATGGTACCTTGACAAAGAACTTAACTGAAAAAAGGTCAGCCAAATCATC TGAGATGTCTCCTGTACCATTGAGGTCAGTTGGCCAAACAGCTAAAGTCCACTTGCATCAGCCACACAGA GTGAGCCAGGTGCTTCAGGTGCCAGCAGTTAACTTGCACCCCATCCGGAAGAAAGGACAAGCCAAAGACC ATGTGCTGAGTACAAGTTTACCTCAGAAAGGTTTAGGACCAACTGAGGAAGTGAGTGTTAAGAAACACAC AGAAGACACTATTTCTGTAGCGTCCTCTCTGCACTCTAGCCCTCCTGCATCTCCTCAGAACTCCCCACGC AAAGGTTATACACTCACACCATCAAGTAAATGTGACAACTTGTCTGACTCCAGCCACAGTGAAATCTCCT CTCGGTCCAGCATTGTGAGCAATGGTTCTGTTGACTCCATGTCTGCAGCTGGGCAGGATGAACGCTGTTC CTCCCACAGTCTGGCAGTTCCTGAGCCCACAGGGGCATTGGAAAAGACAGATCACCCTTCCGGGATCTCA GACCACAGTCAGCTTGCTCACGGGTGGATGCTGTCAAAGCCGTGTCTTATCAAGGGTGTAGCTGTCTCAT CTTCTCTGAGCAGCGAAGAGATGTCTCATGAGCATGTTGTGCTGGAAGCAGCTGACAGTGGGCGGGGCAG TTGGACTTCCTGTTCCAGCAGCTCCCATGACAACTTCCAAAGCCTTCAAAATCAAAAAAGCTGGGACTTC CTGAATTCTTACAGACACATGCATTTAGATGACCCTATTGCAGAAGTTGAGCCCACTGACTGTGAGCCCT GTGCCTGTCCTAAAGGCTGCTCAAGAACTTGTGGCCAGTGCAAAGGCAGCCTAGAGACGAACCAGCTGAG GCAGAGTTGGGCCTCCTCCAGCTCACTCTCGGACACATGTGAACCAAACTACGGGACAGTTAAGCGGAGG GTGTTGGAAAGTGCACCAGCAGAGGCACCTGATGGCTTGGAGCCCAGGGATACCACTGACCCTGTTTATA AAACTGTCACTTCCAGTACAGACAAGGGCTTGATTGAAAACGAGCAGGTGTCAGCAGTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252496
Insert Size	4542 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252496.1, NP_001239425.1
RefSeq Size	7945 bp
RefSeq ORF	4542 bp
Locus ID	192786

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