Ptprs (NM_001252456) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AL022616; PTP; PTP-NU3; PTPNU-3; PTPsigma; Ptpt9; R-PTP-S; RPTPsigma
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229608 representing NM_001252456 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCCACCTGGAGTCCCAGCGTGGTGTCTGTGGTGGGTCCTGTGGGGCTCTTCCTCGTACTGCTGG CCAGAGGATGCTTGGCTGAAGAACCACCCAGGTTTATCAGAGAGCCCAAGGATCAGATTGGAGTGTCGGG AGGCGTGGCCTCCTTCGTGTGCCAGGCCACGGGTGATCCTAAGCCACGGGTGACCTGGAACAAGAAGGGC AAGAAAGTGAACTCACAGCGCTTCGAGACCATTGACTTTGACGAGAGCTCTGGGGCGGTCCTGAGGATCC AGCCACTTCGGACGCCTCGGGATGAGAACGTGTACGAGTGTGTGGCCCAGAACTCGGTGGGCGAAATCAC AATTCATGCAAAGCTCACCGTCCTTCGAGAGGACCAGCTGCCTCCTGGCTTCCCCAACATTGACATGGGC CCCCAGTTGAAGGTTGTAGAGCGCACACGCACAGCCACCATGCTCTGTGCTGCCAGCGGGAACCCGGACC CTGAGATCACCTGGTTTAAGGACTTCCTGCCTGTGGACCCCAGTGCCAGCAACGGGCGGATCAAGCAGCT TCGATCAGGTGCCCTGCAGATTGAGAGCAGCGAGGAGACAGACCAGGGCAAGTACGAGTGTGTGGCCACC AACAGCGCTGGGGTGCGCTACTCATCACCTGCCAACCTCTACGTGCGAGTCCGCCGTGTGGCCCCACGCT TCTCCATCCTGCCCATGAGCCACGAGATCATGCCCGGTGGGAATGTGAATATCACTTGTGTGGCCGTGGG CTCACCCATGCCCTACGTGAAATGGATGCAGGGGGCCGAGGACCTGACGCCTGAGGATGACATGCCCGTG GGTCGGAATGTTCTAGAACTCACGGATGTCAAGGACTCAGCTAACTACACTTGTGTGGCCATGTCCAGCC TGGGTGTGATCGAGGCCGTGGCCCAGATCACTGTAAAATCTCTCCCCAAAGCCCCTGGGACTCCTGTGGT GACGGAGAACACTGCCACCAGTATCACTGTCACATGGGACTCGGGCAACCCTGACCCCGTGTCCTACTAC GTAATTGAGTATAAGTCCAAAAGCCAGGATGGGCCGTATCAGATCAAAGAAGACATCACCACCACGCGCT ACAGCATCGGAGGCCTGAGCCCCAATTCTGAGTATGAGATCTGGGTGTCAGCTGTCAACTCCATTGGCCA GGGCCCTCCCAGTGAATCGGTGGTGACCCGCACAGGTGAGCAGGCACCAGCCAGCGCTCCCAGGAATGTT CAGGCCCGCATGCTCAGCGCCACCACCATGATCGTGCAGTGGGAGGAGCCTGTGGAGCCCAATGGCCTGA TCCGTGGCTACCGTGTCTACTATACCATGGAGCCGGAACACCCAGTGGGCAACTGGCAGAAACACAATGT GGACGACAGTCTCCTGACCACTGTGGGCAGCCTGCTGGAAGACGAGACCTACACCGTGCGCGTGCTCGCC TTCACGTCGGTGGGCGACGGACCACTGTCAGACCCCATCCAGGTCAAGACCCAGCAGGGAGTTCCTGGCC AGCCCATGAACTTGCGGGCTGAGGCCAAGTCAGAGACCAGCATTGGGCTCTCGTGGAGTGCACCACGACA GGAGAGTGTCATTAAGTATGAACTGCTCTTCCGGGAGGGCGACCGAGGCCGAGAGGTGGGGCGAACCTTC GACCCAACCACAGCCTTTGTGGTGGAGGACCTCAAGCCCAATACGGAGTATGCGTTCCGGCTGGCGGCGC GCTCGCCGCAGGGCCTGGGCGCCTTCACCGCGGTCGTGCGCCAGCGCACGCTGCAGGCCATCTCCCCAAA GAACTTCAAGGTGAAGATGATCATGAAGACTTCAGTGCTGCTGAGCTGGGAGTTCCCCGACAACTATAAC TCACCCACACCCTACAAGATTCAGTACAATGGGCTCACCCTGGATGTGGACGGCCGCACGACCAAGAAGC TGATCACACACCTCAAGCCACACACCTTCTATAATTTCGTGCTCACCAACCGTGGCAGCAGCCTGGGGGG CCTGCAGCAGACGGTCACTGCCAGGACCGCCTTTAACATGCTCAGTGGCAAGCCTAGCGTCGCCCCGAAG CCCGACAATGACGGTTTCATCGTGGTCTACCTGCCTGATGGCCAGAGTCCTGTGACCGTGCAGAACTACT TCATTGTGATGGTCCCACTTCGGAAGTCTCGAGGTGGCCAGTTCCCTGTCCTACTAGGTAGTCCAGAGGA CATGGATCTGGAGGAGCTCATCCAGGACATCTCCCGGCTGCAGAGGCGCAGCCTGCGCCACTCCAGACAG CTGGAGGTGCCTCGGCCCTACATCGCCGCTCGATTCTCCATCCTGCCAGCTGTCTTCCATCCTGGGAACC AGAAGCAATATGGTGGCTTTGACAACAGGGGCTTGGAGCCAGGCCACCGCTATGTCCTCTTTGTGCTTGC TGTGTTGCAGAAGAATGAGCCTACATTTGCAGCCAGTCCCTTCTCAGACCCCTTCCAGCTGGACAACCCG GACCCTCAGCCCATTGTGGACGGCGAGGAGGGCCTCATCTGGGTGATTGGGCCTGTGCTGGCCGTGGTCT TCATCATCTGCATCGTGATTGCCATCCTGCTGTACAAGAACAAACCTGACAGCAAACGCAAGGACTCAGA GCCCCGCACCAAATGCTTACTGAACAATGCCGACCTTGCCCCCCATCACCCCAAGGACCCTGTGGAAATG CGACGCATCAACTTCCAGACACCAGGTATGCTCAGCCACCCACCCATCCCCATCACAGACATGGCGGAGC ACATGGAGAGACTCAAAGCCAACGACAGCCTGAAGCTCTCCCAGGAGTACGAGTCCATTGACCCCGGGCA GCAATTCACGTGGGAACATTCGAACCTGGAGGCCAACAAGCCCAAGAACCGCTATGCCAACGTCATCGCC TATGACCACTCACGAGTCATCCTGCAGCCCCTAGAAGGCATCATGGGTAGTGATTACATCAATGCCAACT ATGTGGACGGCTACCGGCGGCAGAATGCATACATTGCCACGCAGGGGCCCCTGCCTGAGACCTTTGGGGA CTTCTGGCGGATGGTGTGGGAGCAGCGATCGGCCACTGTGGTCATGATGACGCGACTGGAGGAGAAATCA CGGATCAAATGTGACCAATACTGGCCTAACCGAGGCACCGAGACATACGGCTTCATCCAGGTCACCCTAC TAGATACCATGGAGCTGGCTACCTTCTGCGTCAGGACTTTTTCTCTACACAAGAATGGCTCTAGCGAGAA GCGTGAGGTGCGACATTTCCAGTTCACGGCATGGCCCGACCACGGGGTACCTGAGTACCCCACGCCCTTC CTGGCATTCCTGCGAAGAGTCAAGACCTGCAACCCGCCTGATGCTGGCCCCATTGTGGTCCACTGCAGCG CGGGTGTGGGGCGCACTGGCTGCTTCATCGTAATTGACGCCATGCTAGAGCGCATCAAGACAGAGAAGAC CGTGGATGTGTATGGACATGTGACACTCATGCGGTCGCAGCGCAACTACATGGTGCAGACAGAGGATCAG TATGGCTTCATCCACGAGGCGCTGCTGGAGGCTGTGGGCTGCGGCAATACCGAGGTCCCTGCTCGCAGCC TCTACACCTACATCCAGAAGCTGGCCCAGGTGGAGCCTGGCGAGCACGTCACGGGCATGGAGCTTGAGTT CAAGAGGCTCGCCAGTTCCAAGGCACACACTTCGCGCTTCATCACCGCCAGCCTGCCTTGCAACAAGTTT AAGAACCGACTGGTGAACATCCTGCCGTACGAGAGCTCGCGTGTCTGCCTGCAGCCCATCCGCGGTGTGG AGGGCTCTGACTACATCAATGCCAGCTTTATCGACGGCTATAGACAGCAGAAAGCCTACATTGCAACACA GGGGCCACTGGCAGAGACCACAGAGGACTTCTGGCGAGCTCTGTGGGAGAACAACTCTACTATTGTCGTA ATGCTCACCAAGCTCCGAGAAATGGGCCGGGAAAAGTGCCACCAGTACTGGCCAGCCGAGCGCTCTGCCC GCTACCAGTACTTTGTGGTTGACCCGATGGCAGAGTATAACATGCCACAGTACATTCTGCGTGAGTTTAA GGTCACAGATGCCCGGGATGGCCAGTCCCGGACCGTCCGACAGTTCCAGTTCACGGACTGGCCAGAGCAG GGTGCACCCAAGTCAGGGGAAGGCTTCATTGACTTCATCGGCCAAGTGCATAAGACCAAGGAGCAGTTTG GCCAGGACGGACCCATCTCAGTGCACTGCAGCGCCGGAGTGGGCAGGACCGGAGTGTTCATCACCCTGAG CATCGTGCTTGAGCGGATGCGCTACGAGGGCGTGGTGGACATTTTCCAGACAGTGAAGGTGCTTCGGACC CAGAGGCCTGCCATGGTGCAGACAGAGGACGAGTACCAGTTCTGCTTCCAGGCGGCTTTGGAATACCTGG GCAGTTTTGATCATTATGCAACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252456
Insert Size	4506 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252456.1, NP_001239385.1
RefSeq Size	5618 bp
RefSeq ORF	4506 bp
Locus ID	19280
UniProt ID	B0V2N1
Gene Summary	Cell surface receptor that binds to glycosaminoglycans, including chondroitin sulfate proteoglycans and heparan sulfate proteoglycans (PubMed:19833921, PubMed:21454754, PubMed:22406547). Binding to chondroitin sulfate and heparan sulfate proteoglycans has opposite effects on PTPRS oligomerization and regulation of neurite outgrowth (PubMed:21454754). Contributes to the inhibition of neurite and axonal outgrowth by chondroitin sulfate proteoglycans, also after nerve transection (PubMed:15797710, PubMed:19833921, PubMed:19780196, PubMed:21454754, PubMed:22519304, PubMed:22406547). Plays a role in stimulating neurite outgrowth in response to the heparan sulfate proteoglycan GPC2 (PubMed:21454754). Required for normal brain development, especially for normal development of the pituitary gland and the olfactory bulb (PubMed:10080191). Functions as tyrosine phosphatase (PubMed:7529177). Mediates dephosphorylation of NTRK1, NTRK2 and NTRK3 (By similarity). Plays a role in down-regulation of signaling cascades that lead to the activation of Akt and MAP kinases (PubMed:15797710). Down-regulates TLR9-mediated activation of NF-kappa-B, as well as production of TNF, interferon alpha and interferon beta (PubMed:26231120).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and lacks four consecutive exons in the coding region, but maintains the reading frame, compared to variant 1. Variants 2 and 4 encode the same isoform (2), which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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