Shroom2 (NM_001290684) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4832440C16; Apxl; C630003H05Rik; Shrm2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229602 representing NM_001290684 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCGCCGAGCCCCGTGCACGGCCCGAGCGCCTGGCCGAAGCTGAGGCGCCGGCGACCGACGGCG TCCGCCTGGTGGAGGTGCAGCTGAGCGGCGGCGCCCCTTGGGGCTTCACCTTGAAGGGCGGCCGCGAGCA TGGCGAGCCTCTGGTCATCACCAAGATTGAAGAAGGAAGCAAAGCTGCAGCAGTGGACAAGCTACTTGCT GGAGATGAGATAGTGGCCATTAATGATGTGAGTCTCTCAGGGTTCAGACAAGAAGCAATTTGCCTGGTGA AAGGCTCCCACAAGACCCTGAAGCTTGTGGTCAAAAGGAAAAGTGATCCAAGTTGGAGGCCCCACTCCTG GCACGCTACCAAGTACTTTGATGTCCACCCTGAGCCAGCAGCCTCACTGTTTCTGAACACCAGCGGTTCC CCTTCCTGGAAGAGCCAGCACCAGGCTAGTTCTTCCTCTCATGATTTATCTGGCTCATGGGAGCATACAA GCCTACAGCGCACTTCTGACCACTTCAGCTCCATGGGCAGCATAGACAGCCTCGACCACAGCTCCCAACT CTACCCATCTGGACACCTCTCATCTGCCAAGTCCAATAGCAGCATTGACCACCTGGGAGGCCACAGCAAG CGAGACTCAGCTTACGGCTCCTTTTCCACATGCTCCAGCACCCCTGACCACACCTTGCCCAAGGCTGATG CCTCTTCCACTGAGAACATTCTCTATAAAGTTGGCCTTTGGGAGGCCTCCAGACCAGGCAGCAGCCGACA AAGCCAGTCTACAGGTGATCCTCAGGGACTGCAGGACAGGCCATCATGTTTCATACCTAGGGTTCCTGGT AACAGTAGTAAAAGTCCAAGGCCGGAGGATAATGTTGAACCCAAAATAGCCACTCATGGGAGATCCAATT TTGGGCCTGTGTGGTATGTTCCTGACAAGAAAAAAGCCCCTTCTCCCCCACCTCTTGGACTTCCTTTGCG CAGTGATAGCTTTTCTGTGGCAGCCAGGGGCCATGAAAAAGCCCGGGGCCCTCCATTCTCAGACTTGGCC AGTATGCAGCACTTTATCACCCTGCCCCATGTGCAACCCCGGGGGGACCATAGAATGGAAACTACAGATC GTCAGTGGAAGCTCACACACCTAAGCAGTGGAAAAGAAATAGGAAATGTGGGTTACCAGTCAGAAGGCCA CCTGGATTGCCGTTGGCTATGCTCTGATGATAGAGCAGGTAGGCCCTCAGGGCCTCCGGGCAGGCTCCAG TTCTCCGATGTGCACTTTCTGAAGTCTTACCACGGGAGCCAGCACCAACAGCAGTGCAGTGACGAGAGCC CAAGGGCCCCCTCATCACCAAGGGAACTGCTTCATATAACTCCTGGTGGCGGTCTGCAAGAGCCTCCTGA ACCATCTCAGGATGACAACCCTACTCAGGTGAGGTGGCCTGGTTCTGCTCACCAGAAGCTAGATGACAGA GGGCGGAGCCACTATTTTCCTGGGTCCCTCAGGCAGCCTGTACAGGGGAGTGCCCAGGTTGTGATACCCC GAGGTGACTATTGGCATTCAGATACAACTCCTGTGGACCTTGAATATCCTCTCTTACGCCCAGTAGGACA GAGGACCTACCTACAGCAGCATGAGGAGACTCCGGCCTCTCATGAAAAAGAGGGGTATCACCAGCTAAAT GCAGGGATTGAAGGCTGCTGCTCTGGAATCCAAGAGCCTCCTAGAGCCAGCCGCACTGTGAGAACTGGTC TGCAGTGTCCTAGCAATGACTTCAAATTAGTGGATGGAGAGAGTGGGAGAATTTCTCGTCAGAGGACACC CATGCTGCATTCTCTGACCCAAGATGGTACATGGAGACCCGGGAACAGCAAGGATTGTGGAAATGATAAG CCACCACTGTTTGATGCCCAGGTGGGTAAACCCACACGGAGGAGTGACCGTTTTGCCACAACACTGAGGA ATGAGATCCAAATGCGTAGAGCAAAGCTGCAGAAGAGCAAAAGTACAGTGACACTAGCTGGAGACAGTGA AGCTGAAGATTGTGCTGGAGACTGGAGAGCTGATGTGGGGGCTGTCCCAGAAGGTTCCTTCCCCAGCACC TATAAAGAGCACCTGAAGGAGGCCCAGACACGTGTTCTGAAGGCCACCTCTTTCCAACGCCGAGATTTAG ATCCCACCCCAGCAGATCAGTATTCAGGACCATCAGAACACAGGACTTTTGACCACAGTGCCTCATCTTC TTTATCTTCTTTCCCTGGGGAGCCAGACTCTGCCCCACGCTTCTGTGAGACAGGTCTGGCCAAGGCACCC TCTTCTGGAGTTGGTGTACCCCACGTTCTTCGAATTGGAGGCCGGAAACGGTTCACAGCAGAGCAGAAAC TAAAGTCCTATTCTGAGCCAGAGAAAATAAATGAAGTGGGGCTCTCAGGGGACCACCGTCCTCATCCTAC TGTCAGGACACCTGAGGATACAGTGGGTACATTTGCTGACAGGTGGAAGTTTTTTGAGGAAACAAGCAAA TCTCTTCTCCAGAAGGCAGGCCATAGGCAAGTTCACTGTGGGCTCCCTAGAGAGAAGGCTGAGAGGCCAC AGACAGGGCACCATGAATGTGAGAGTACAGAGCCCTGGTTCCAGAAGAGGTCACTGGCCACCTCCTGCGG AGAGATCCTCAGTGATAGAAAAGTAGAAAAGGCCTCAGAGAAATTGAACCCACCCAGAAGGCTTGGAACC TTCGCAGAATATCAAGCATCTTGGAAGGAACAGAAGAAACCTCTGGAAGCCAGGAGTTCTGGACGATACC ATTCAGCAGATGATATTCTGGATGCTGGTCTAGATCAGCAGCAGAGGCCACAGTACATTCATGAGCGGTC TCGTTCATCACCGTCCACAGATCACTACTCACAGGAAGTGCCTGTTGAACCAAACAGGCAGGCAGAGGAC TCTGGTGACCACAAAGAAGCAATTCTCTGTACACTACAAGCTGAGGAGGGATGCTCTGCTCCAAGTGCCC AGCCGCAAGACAGCCAGCATGTGAATGAAGACACAACTTTCCCTCAACCAGAAACCCAGCTCTCTTCCAA GTGTCAACACCTACAGACGTCAGCCATGGAAACTTCTCGCTCCCCTTCGCCTCAGTTTGCCCCACAGAAG CTGACAGATAAACCTCCCCTGCTTATCCATGAAGACAACTCAGCAAGAATCGAGCGGGTGATGGACAACA ACACCACTGTGAAGATGGTGCCCATAAAAATTGTGCACTCAGAAAGCCAGCCCGAGAAGGAGAGTCGTCA GAGTCTCGCGTGCCCAGCTGAGCTGCCCCCACTGCCCAGTGGGCTGGAGAGGGACCAGATCAAGACACTG AGTACATCAGAGCAATGCTACTCCCGCTTCTGTGTGTACACACGACAGGAGGTGGAAGCTCCTCATAGAG CCCGCCCTCCAGAGCCCCGGCCACCCAGCACGCCTGCACCTCCTGTCAGAGATAGCTGTTCCTCCCCTCC CTCACTCAACTATGGGAAGGCCAAGGAGAAAACCATGGATGACTTGAAGTCTGAAGAATTAGCCAGGGAG ATTGTGGGAAAGGACAAGTCTTTGGCTGACATCCTGGACCCCAGTGTGAAGATCAAAACTACCATGGATC TGATGGAGGGAATTTTTCCCAAAGATGAGTACCTCCTAGAGGAAGCTCAGCAGCGGAGAAAGCTGCTCCC CAAAGTCCCCTTACCCAGAGTCACAGAGGACAAGAAACAGGACCCAGGTATGCCAGGGGTTGTGTCCTTG GCCACCAATTCTACCTATTACAGCACATCAGCCCCCAAAGCAGAGCTTCTTATCAAGATGAAGGACCTGC AGGAGCCTGAAGAGTATTCAGCAGGTGACTTGGATCATGACCTTTCTGTTAAGAAGCAAGAGCTCATTGA CAGTATCAGCCGCAAGCTGCAGGTGCTCCGGGAAGCACGTGAGAGCCTGCTGGAAGACATCCAAGCCAAC AATGCTCTTGGGGATGAGGTGGAAGCCATTGTGAAAGATGTCTGCAAGCCCAATGAGTTTGACAAGTTCC GGATGTTCATTGGAGACCTGGACAAAGTGGTGAACCTCCTGCTGTCACTGTCAGGACGCCTGGCCCGTGT GGAAAATGCCCTTAATAATTTAGATGACAATCCTTCTCCTGGAGATCGGCAGTCACTGTTGGAGAAACAG AGAGTCCTAACTCAGCAGCACGAGGATGCCAAGGAGCTTAAAGAGAACCTGGACCGCCGTGAGCGCATTG TGTTCGACATCCTGGCTACCTACCTCAGCGAGGAGAACCTGGCTGACTATGAGCACTTCGTGAAGATGAA GTCAGCTCTCATCATTGAGCAGCGAGAGCTGGAAGATAAAATCCACCTGGGTGAAGAGCAGCTCAAGTGT TTGTTTGACAGCCTACAGCCTGAGAGAAGCAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290684
Insert Size	4446 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290684.1, NP_001277613.1
RefSeq Size	7501 bp
RefSeq ORF	4446 bp
Locus ID	110380
UniProt ID	A2ALU4
Gene Summary	May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.