Syngap1 (NM_001281491) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gm1963; Syngap
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229566 representing NM_001281491 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGGTCTCGAGCCTCCATCCATCGGGGGAGCATCCCCGCGATGTCCTATGCCCCCTTCAGAGATG TACGGGGACCCCCTATGCACCGAACCCAATACGTTCATTCCCCGTACGATCGTCCTGGCTGGAACCCTCG GTTCTGCATCATCTCGGGGAACCAGCTGCTCATGCTGGATGAGGATGAGATACACCCCCTTCTGATCCGC GACCGGAGGAGCGAGTCCAGCCGAAACAAACTGCTGAGACGCACCGTCTCTGTGCCAGTGGAGGGGCGGC CCCACGGCGAGCATGAATACCACTTGGGTCGCTCGAGGAGGAAGAGTGTCCCAGGGGGGAAACAGTACAG CATGGAGGCCGCCCCCGCTGCGCCCTTCCGGCCCTCGCAAGGCTTCCTGAGCCGGAGGCTAAAAAGCTCT ATCAAACGTACAAAGTCACAACCCAAACTTGACCGGACCAGCAGCTTTCGACAGATCCTGCCTCGCTTCC GAAGTGCTGACCATGACCGGGCCCGGCTGATGCAGAGCTTCAAGGAGTCACATTCCCACGAGTCCCTGCT GAGTCCCAGTAGTGCTGCTGAGGCCTTGGAGCTCAACCTGGATGAAGACTCCATTATCAAGCCAGTACAC AGCTCCATCCTGGGTCAGGAGTTCTGCTTTGAGGTAACAACATCATCTGGAACAAAATGCTTTGCCTGTC GGTCTGCAGCTGAAAGGGACAAATGGATTGAGAATCTGCAGAGGGCTGTAAAACCCAACAAGGACAACAG CCGCCGAGTAGATAACGTGCTGAAGCTATGGATCATAGAGGCTCGAGAGCTGCCCCCCAAGAAGAGATAT TACTGTGAGCTGTGCCTGGACGACATGCTGTATGCACGAACCACCTCCAAGCCCCGCTCGGCTTCAGGAG ACACCGTCTTTTGGGGCGAGCACTTTGAGTTTAACAACCTGCCTGCCGTCCGGGCCCTTCGGCTGCATCT GTACCGTGACTCAGACAAAAAGCGGAAGAAGGACAAGGCTGGCTACGTTGGCCTGGTGACTGTTCCAGTG GCCACCCTAGCTGGGCGCCACTTCACAGAGCAGTGGTACCCCGTGACCTTGCCGACAGGCAGTGGGGGCT CTGGGGGCATGGGCTCGGGGGGAGGAGGAGGGTCAGGGGGTGGCTCAGGGGGCAAAGGGAAAGGAGGTTG TCCTGCTGTGAGACTGAAAGCCCGTTACCAGACGATGAGCATCCTGCCCATGGAGCTGTATAAGGAGTTT GCAGAGTATGTGACCAACCATTACCGGATGCTATGTGCAGTGCTGGAGCCTGCCCTGAATGTCAAAGGCA AGGAAGAGGTGGCCAGTGCACTGGTTCACATCCTGCAGAGCACAGGCAAGGCCAAGGACTTCCTTTCAGA CATGGCCATGTCAGAGGTAGACCGATTCATGGAGCGGGAACACCTCATATTCCGAGAGAACACGCTCGCC ACTAAAGCCATAGAAGAGTATATGAGACTGATTGGCCAGAAATACCTCAAGGATGCCATTGGGGAGTTCA TTCGTGCTCTGTATGAATCTGAGGAGAACTGTGAAGTAGACCCCATCAAGTGCACAGCGTCCAGTCTGGC AGAGCACCAGGCCAACCTGCGGATGTGCTGTGAGTTGGCCCTGTGCAAGGTGGTCAACTCCCATTGCGTG TTCCCGAGGGAGCTGAAGGAGGTGTTTGCATCTTGGCGGCTGCGCTGCGCAGAGCGGGGCCGAGAGGACA TTGCTGACAGGCTGATCAGCGCCTCGCTCTTCCTGCGCTTCCTCTGCCCGGCCATTATGTCGCCCAGTCT ATTTGGGCTTATGCAGGAGTACCCAGATGAGCAGACCTCACGAACCCTCACCCTCATCGCCAAGGTCATC CAGAACCTGGCCAACTTTTCCAAGTTTACCTCAAAGGAGGACTTCCTGGGCTTCATGAATGAGTTTCTGG AGCTGGAGTGGGGCTCTATGCAGCAGTTCTTGTATGAGATATCCAACCTGGACACCTTGACCAACAGCAG CAGTTTTGAGGGCTATATAGACTTGGGCCGCGAGCTCTCCACACTTCACGCCCTGCTCTGGGAGGTGCTG CCCCAGCTCAGCAAGGAAGCCCTCCTGAAGCTGGGCCCACTGCCCCGGCTCCTCAATGACATCAGCACAG CCCTGAGGAACCCTAACATCCAAAGGCAGCCAAGCCGCCAGAGTGAACGGACTCGGTCTCAGCCCATGGT GCTGCGAGGGCCATCAGCCGAGATGCAGGGCTACATGATGCGGGACCTCAATAGCTCCATCGACCTTCAA TCCTTCATGGCTCGAGGCCTCAACAGCTCTATGGACATGGCTCGCCTCCCCTCCCCAACCAAGGAAAAAC CACCACCACCACCACCCGGTGGGGGTAAAGACCTTTTCTATGTGAGCCGGCCACCACTGGCCCGGTCCTC CCCAGCATACTGCACGAGCAGCTCGGACATCACAGAGCCAGAGCAGAAGATGCTGAGTGTCAACAAGAGT GTGTCCATGCTGGACCTTCAGGGCGACGGGCCTGGAGGTCGCCTTAACAGTAGCAGTGTTTCCAACCTGG CAGCTGTTGGGGACTTGTTGCATTCCAGCCAGGCCTCGCTGACAGCAGCTTTGGGGTTGCGGCCTGCACC TGCCGGGCGCCTCTCCCAGGGGAGTGGCTCTTCCATCACAGCAGCTGGCATGCGCCTCAGCCAGATGGGG GTCACTACAGATGGTGTCCCCGCCCAGCAACTGCGCATCCCCCTTTCCTTCCAGAACCCTCTCTTCCATA TGGCTGCTGATGGGCCAGGGCCCCCAGCAGGCCATGGAGGGAGCAGTGGTCATGGTCCACCTTCCTCCCA TCACCACCACCACCACCATCACCACCACCGAGGGGGAGAACCCCCAGGGGACACTTTTGCCCCATTCCAT GGCTATAGCAAGAGCGAGGACCTCTCTTCAGGGGTCCCTAAGCCCCCCGCCGCCTCCATCCTTCACAGCC ACAGCTACAGCGACGAGTTTGGACCCTCTGGCACTGATTTTACCCGCCGGCAGCTCTCGCTTCAGGACAG TCTACAGCACATGCTCTCCCCTCCCCAGATTACCATCGGTCCCCAGAGGCCAGCTCCCTCAGGGCCGGGA GGGGGCAGCGGCGGGGGCAGCGGTGGGGGCCAGCCACCCCCCTTGCAGAGGGGCAAGTCTCAACAGTTGA CAGTGAGTGCCGCCCAGAAACCTCGGCCGTCTAGCGGGAACCTGTTGCAGTCCCCGGAGCCAAGCTACGG ACCTGCCCGTCCTCGGCAGCAGAGCCTCAGCAAAGAGGGCAGCATTGGGGGCAGCGGGGGCAGCGGGGGC GGAGGGGGTGGGGGGCTCAAGCCCTCCATCACCAAGCAGCATTCCCAGACTCCATCCACGCTTAACCCCA CAATGCCAGCCTCGGAGCGGACCGTAGCCTGGGTCTCCAACATGCCTCACCTGTCGGCTGACATCGAGAG TGCACACATCGAGCGAGAAGAGTACAAGCTCAAGGAGTACTCCAAGTCCATGGACGAAAGCCGGCTGGAC AGGGTGAAGGAGTATGAGGAGGAGATACATTCGCTGAAGGAGAGGCTACACATGTCCAACCGGAAGCTGG AAGAGTATGAGCGGAGGTTGCTGTCCCAGGAAGAACAGACCAGCAAGATCCTGATGCAGTACCAAGCCCG CCTGGAGCAGAGTGAGAAGCGCTTGAGACAGCAGCAGGTGGAGAAGGACTCCCAGATCAAGAGCATCATT GGCAGGCTGATGCTGGTGGAGGAGGAGCTGCGTCGGGACCACCCCGCCATGGCTGAGCCGCTGCCTGAGC CCAAGAAGAGGCTGCTCGACGCTCAGGAGAGGCAGCTTCCCCCCTTGGGTCCAACAAACCCGCGTGTGAC GCTGGCCCCACCTTGGAACGGCCTGGCCCCCCCAGCCCCACCCCCCCCACCCCGGCTGCAGATCACAGAG AACGGCGAGTTCCGGAACACCGCAGACCACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281491
Insert Size	4023 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281491.1, NP_001268420.1
RefSeq Size	4023 bp
RefSeq ORF	4023 bp
Locus ID	240057
UniProt ID	F6SEU4
Gene Summary	This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. The N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. The encoded protein negatively regulates Ras, Rap and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking to the postsynaptic membrane to regulate synaptic plasticity and neuronal homeostasis. Homozygous null mutations result in early post-embryonic lethality, while heterozygous mutant mice display a variety of phenotypes that include learning and memory defects, hyperactivity, and audiogenic seizures. [provided by RefSeq, Nov 2016]

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