Ttc21b (NM_001290669) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2410066K11Rik; aln; mKIAA1992; Thm1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229533 representing NM_001290669 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCCCAGGGGCTGAAGACGCTGATTAACTACTATTGTCAAGAGCGCTATTATCACCACGTGCTCC TTGTTGCCAGTGAAGGGATGAAGAAGTATAGCAGCGACCCAGTCTTCCGATTTTACCACGCCTACGGCAC ACTGATGGAGGGTAAAGCGCAAGAAGCCCTTCGGGAGTTTGAGGCCATTAAAAACAAACAAGACGTGTCA CTGTGTTCTCTGATGGCGCTGATGTATGTCCATAAAATGAGCCCCAATCCAGACAGAGAAGCTATCCTGG AATTAGATACCAAAATGAAGGAGCAACGCAAGGAGGCTGGACGCAAAGCCCTGTACCATGCGGGCCTGTT TTTATGGCACATTGGTCGTCATGACAAGGCGAGAGAATATATTGACAGAATGTCAAAAATGCCACATGAT AGCAATGAGGGGCCGATTTTGAAAGCGTGGCTTGATATTACAAGAGGGAAGGAACCTTATGCTAAAAAAG CCCTGCGGTATTTTGAGGAGGGATTACAAGATGGAAATGATATTTTCGCTCTTCTGGGTAAGGTTCTGTG CCTTGAGATTCGACAGAATTATTCCGGAGCTCTGGAGACCGTGAGCCAGATAATTGTAAACTTTCCAAGC TTCCTTCCTGCCTTTGAGAAGAAAATGAAATTACAACTGGCTTTACAGGATTGGGATCAGACAGTGGAGA CAGCACAAAGGCTGCTGCTTCAAGACAATCACAACGTGGAGGCGCTGAGGATGCTGGCTCTGTATTATCT GTGTAGGGAAGGGGACGTAGAGAAGGCTGCTACCAAGCTGGAAAATTTAGGAAATGCATTGGATGTCATG GAACCACAGAATGCTCAACTTTTTTATAAGATTACACTAGCCTTCAGCAGAACGTGTGGACGTAATCAAC TCATTCTCCAGAAAGTTCAAAGTTTCCTAGAAAAAGCATTTAGTTTAACTCCCCAGCAAGCAGAAATTGC TACAGAGCTCGGCTACCAAATGATTCTCCAAGGCAAGGTCAAGGAGGCCTGGAAGTGGTACAGGACCGCC ATGACGCTGAATGAGAGCAACATCTCTGCTGTCACCGGACTTATCCGATGTCAGTTAATAGAGGGGCAGT TGCAAGATGCAGACCAGCAGTTGGAGTTCTTCAGTGAATTCCAGCAGTCAATGGGGAAATCTGCGGAATT AATGTATTTGCATGCGGTTCTTGCTACGAAAAAAAATAATCGTCAGGATGAAGTTATAAATTTGTTGAAT GATGTTGTGAATACTCACTTTTCACACTTGGAAGACCTCCCGCTCGGCATACAGTATTTTGAGAAGCTCA ACCCTGACTTCTTACTAGAAGTTGTTACCGAGTATCTGAATCTCTGTCCAATTCAGCCTGCAGGTCCTGG ACAACCTCTTTCTCCAGTTCTCAGACGTTGTTCTTCAGTCCTGGAGACGATTATAAGAAGTGTACCAGGT CTTCCACAAGCTGTCTTCCTAATGGCCAAAGTGAAGTATTTGTCAGGTGACACTGAAGCGGCATACAACA ATCTGCAGCACTGCCTCGAGCACAGCCCCTCCTATGCGGAGGCTCACCTCCTGATGGCACAGGTGTACCT GTCCCAAGACAAAGTCAAGCTGTGTTCGCAGTCGCTTGAACTTTGTCTGAGCTACAATTTTAATGTGAGA GAGTACCCATTATATCATTTAATCAAAGCACAGTCACAAAAGAAAATGGGGGAAGTAGCAGAAGCAATTA AAACTCTGCACATGGCAATGAACCTGCCAGGAATGAGGAGAAGCAGAGCCTCCTCGAAATCAAAGCACAG AACTGAGGTGGACGCGAGCCATCGGTTATCCATCTTCCTGGAGTTGGTGGAGGTCCACCGCTTAAATGGA GAACAGCATGAGGCAGCAAAGGTTTTACAGGATGCCATCCATGAGTTTTCGGGAACTTGTGAAGAATTAC GTGTCACTATTGCTAATGCAGACCTGGCTCTGGCCCAAGGAGATACGGATCGTGCATTAAGCATGCTTCG AAATGTCACAACCGAACAGCCTTATTTTATAGAGGCCAAAGAAAAGATGGCAGATATTTATCTGAAGCAT AGGAAAGAGAAAATGTTGTATATCACTTGTTACAGAGAAATTGCTGAGAGAATGCCCAGTCCCCGGTCTT TCCTGCTCCTCGGCGATGCGTACATGAACATTCAGGAGCCAGAAGAAGCCATAGTGGCCTATGAGCAAGC ACTCAATCAGAACCCCAAAGATGGAACACTGGCAAGAAAAATTGGAAAAGCACTTGTCAAAACTCACAAT TACTCAAAGGCAATCACCTATTATGAAGCGGCTCTGAAAAGTGGACAGCAGAATTGCCTTTGCTATGACC TGGCCGAGCTGCTACTGAGACTGAAGCTGTACGAGAAAGCAGAGAAAGTGCTCCAGCACTCTCTAGCTCA TGAGCCTGTAAGTGAGTTGTCAGCTCTCATGGTGGATGGGCGGTCCCAAGTCCTTCTTGCAAAAGTTTAC TCTAAAATGGAGAGACCCAGCGACGCGATTGCCGCATTACAGCAGGCTCGAGAGCTGCAGGCTCGGATAT TGAAGCGAGTTCAGATGGAGCAGCCAGATGCAGTTCCTTCACAGAAACACTTCGCAGCTGAAATTTGTGC AGAGATTGCGAAACACTCCGCCGCTCAGCGAGACTATGAGAAAGCAATTACATTTTATAGAGAAGCCCTG GTTCATTGTGAAACAGACAGTAAGATAATGTTGGAACTGGCGCAGTTATACCTGGCCCAAGAAGACCTTG ACGCCTCCCTGCGACACTGTGCGCTGCTTCTCCAGAGGGACCAGGACAATGAACCTGCCACCATGTTGAT GGCCGACCTCATGTTCAGAAAGCAAGACTATGAACAAGCAGTGTATCATCTGCAGCAGCTTTTAGACCGG AAACCAGATAATTTTATGACTTTGTCCCGTTTGATTGATCTCCTGAGAAGATGTGGGAAACTTGAGGATG TTCCGAGATTTTTCTTGATGGCTGAGAAACACAACTCCAGAACAAAATTGGAGCCAGGATTTCAGTACTG TAAAGGATTACATTTTTGGTATACTGGAGAACCAAATGATGCCCTTCGACATTTTAATAAAGCTCGGAAA GATAGTGACTGGGGTCAGAATGCACTTTATAATATGATAGAAATCTGCCTAAATCCAGACAATGAAACTA TTGGAGGTGAAGTGTTTGAAAACCTGAATGGAGACCTGGGCACTTCCCCTGAGAAGCAGGAGTCTGTGCA GTTAGCAGTGAGGACAGCAGAAAAGCTCCTTAAGGAACTGAAGCCTCAGACCGTCCAGGGCCGCCTGCAA CTCCGCATAATGGAAAACTGCTGCTTGATGGCCACCAAGCAGAAGTCCAGTGTGGAGCAGGCACTGAACA CCTTCACCGAAATCGCAGCCTCCGAGAAGGATCATATCCCAGCTCTCTTGGGAATGGCAACGGCTTATAT GATCTTGAAACAGACTCCAAAAGCCAGAAACCAGCTGAAGCGGATTGCAAAAATGCCTTGGAATCCTATT GAAGCCGAGGATCTGGAGAAGAGCTGGCTGCTGCTTGCTGATATTTATATCCAGTCAGCAAAATATGACA TGGCAGAGGAATTACTAAAGCGGTGTCTCTGCCATAACCGGTCCTGCTGCAAAGCTTATGAATATATGGG GTACATTATGGAGAAAGAGCAAGCATACACAGATGCGGCCTTCAACTATGAGATGGCATGGAAACATAGC AACCAGACAAATCCTGCAGTGGGTTCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290669
Insert Size	3810 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290669.1, NP_001277598.1
RefSeq Size	4402 bp
RefSeq ORF	3810 bp
Locus ID	73668
Gene Summary	Component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs). Essential for retrograde trafficking of IFT-1, IFT-B and GPCRs (By similarity). Negatively modulates the SHH signal transduction (PubMed:18327258).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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