Apaf1 (NM_009684) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6230400I06Rik; Apaf-1; Apaf1l; fog; mKIAA0413
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229517 representing NM_009684 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCAAAGGCCCGCAATTGTTTGCTTCAACATAGAGAAGCTTTGGAAAAGGACATCAAAACATCCT ACATCATGGATCACATGATCAGTAATGGCGTCTTGTCAGTGATAGAGGAGGAGAAGGTCAAAAGTCAGGC CACTCAATATCAACGAGCAGCCGCTTTAATTAAAATGATACTTAATAAAGACAACTGTGCCTACATTTCA TTCTACAACGCTCTGCTACACGAGGGCTATAAGGACCTTGCTGCGCTTCTGCAGAGTGGCCTTCCTCTTG TGTCGTCTTCCAGTGGCAAGGACACAGATGGTGGAATAACTTCATTTGTAAGGACAGTGCTGTGTGAAGG TGGAGTACCCCAGAGGCCGGTTATTTTCGTTACTAGAAAGAAGCTGGTTCATGCGATTCAGCAGAAGCTC TGGAAACTGAATGGAGAACCAGGGTGGGTCACCATCTATGGGATGGCAGGCTGCGGCAAGTCTGTGTTAG CTGCGGAAGCCGTTCGAGATCACTCCCTCTTAGAAGGTTGCTTTTCAGGGGGTGTACACTGGGTTTCCAT TGGAAAACAAGACAAATCTGGGCTTCTCATGAAACTGCAGAATCTGTGCATGCGCTTGGACCAAGAAGAG AGTTTCTCTCAGAGGCTTCCACTTAATATTGAGGAGGCCAAAGACCGCCTCCGTGTTCTGATGCTGCGCA AACACCCAAGGTCTCTGTTGATCTTGGATGATGTTTGGGATCCTTGGGTGTTAAAAGCTTTTGACAATCA GTGTCAGATTCTTCTTACAACCAGAGATAAGAGTGTTACAGATTCAGTAATGGGTCCTAAGCATGTTGTC CCTGTGGAGAGTGGTCTAGGGAGAGAGAAAGGACTTGAGATCTTGTCACTTTTTGTTAACATGAAGAAAG AAGATCTGCCAGCGGAGGCTCACAGTATTATAAAGGAATGCAAAGGTTCTCCTCTTGTAGTGTCTTTAAT TGGTGCGCTTTTACGTGATTTTCCCAATCGCTGGGCATACTACCTCAGACAGCTTCAGAATAAGCAGTTT AAGAGAATAAGGAAGTCTTCATCTTATGATTATGAGGCTCTAGATGAAGCCATGTCGATAAGTGTTGAAA TGCTCAGAGAAGACATCAAAGACTATTACACAGACCTTTCCATCCTTCAGAAGGACGTCAAGGTACCTAC AAAGGTGTTGTGCGTTCTCTGGGACTTGGAAACGGAAGAAGTTGAAGACATCCTGCAGGAGTTCGTTAAT AAGTCTCTCTTATTCTGTAATCGGAATGGAAAGTCATTTTGTTATTATTTACATGATCTTCAAGTAGATT TTCTTACAGAGAAGAATCGCAGTCAGCTTCAGGATCTGCACAGGAAGATGGTCACTCAGTTTCAGAGGTA TTACCAGCCCCACACGCTGTCTCCAGACCAGGAGGACTGCATGTATTGGTACAACTTCCTAGCCTATCAC ATGGCTAGTGCCAATATGCACAAAGAACTTTGTGCTTTAATGTTTTCCCTGGACTGGATTAAAGCAAAAA CAGAACTTGTCGGCCCTGCCCATCTGATTCACGAGTTCGTGGCATATAGGCATATATTGGATGAAAAGGA TTGTGCAGTCTGTGAGAATTTTCAAGAGTTTTTATCTTTAAATGGACACCTCCTTGGACGACAGCCATTT CCTAATATTGTACAGCTGGGCCTCTGTGAACCAGAAACTTCCGAAGTTTATCGACAAGCAAAGCTGCAGG CCAAGCAGGAGGGGGATACTGGGCGCCTTTACCTGGAATGGATAAACAAAAAAACTATCAAGAATCTGTC CCGCTTAGTCGTCCGCCCCCACACAGATGCTGTTTACCACGCGTGTTTTTCTCAGGATGGTCAGAGAATA GCTTCTTGTGGGGCTGATAAAACCTTACAGGTGTTCAAAGCCGAGACAGGAGAGAAACTTCTTGACATCA AAGCTCACGAAGATGAGGTGCTTTGCTGCGCGTTCTCCTCAGACGACAGTTACATAGCGACCTGCTCAGC GGATAAGAAGGTTAAGATTTGGGATTCTGCGACTGGGAAGCTTGTGCACACCTACGACGAGCACTCGGAG CAAGTCAATTGCTGCCATTTCACCAACAAGAGTAACCACCTTCTCTTGGCCACTGGGTCAAATGATTTCT TCCTCAAGCTCTGGGATTTGAATCAAAAAGAATGTCGAAATACCATGTTTGGTCACACGAACTCAGTCAA CCACTGCAGGTTCTCACCAGACGATGAGCTCTTGGCTAGCTGCTCAGCTGACGGGACTTTAAGGCTTTGG GATGTGAGATCAGCAAACGAGAGGAAAAGCATTAATGTGAAGCGCTTCTTCCTGAGTTCAGAAGACCCTC CAGAGGATGTGGAGGTGATCGTGAAGTGTTGTTCCTGGTCTGCAGATGGTGACAAAATAATAGTGGCAGC AAAAAACAAAGTCCTCCTTTTTGATATTCATACTAGTGGCCTATTGGCAGAGATCCACACAGGCCATCAC AGCACCATCCAGTACTGTGACTTCTCCCCCTATGACCATTTGGCTGTGATTGCCCTGTCTCAGTACTGTG TGGAGTTGTGGAACATAGACTCCCGCCTAAAGGTGGCCGACTGCAGAGGACATTTGAGTTGGGTTCACGG TGTGATGTTTTCTCCCGATGGCTCCTCATTTTTGACAGCTTCTGATGACCAAACAATAAGGGTCTGGGAG ACAAAAAAGGTATGCAAGAACTCTGCCATCGTGCTAAAGCAGGAAATAGACGTCGTGTTTCAAGAGAACG AAACGATGGTCCTTGCAGTTGACAACATAAGAGGCCTGCAACTCATTGCTGGAAAAACAGGCCAGATTGA TTACCTGCCTGAAGCCCAAGTGAGTTGCTGCTGCCTCAGTCCACACCTTGAGTACGTGGCATTCGGAGAT GAAGATGGAGCCATTAAGATTATAGAACTTCCAAACAACAGAGTCTTCAGTTCTGGGGTTGGGCACAAGA AAGCTGTGCGGCACATCCAGTTCACAGCTGATGGGAAGACACTGATTTCAAGTTCTGAAGATTCTGTGAT TCAGGTATGGAATTGGCAGACAGGGGACTATGTATTTTTGCAAGCCCACCAGGAAACGGTAAAGGACTTC AGGCTCCTCCAAGATTCAAGATTGCTTTCTTGGTCATTTGATGGAACGGTGAAGGTGTGGAATGTCATTA CCGGAAGAATAGAAAGAGACTTTACTTGTCATCAGGGCACAGTGCTTTCCTGTGCTATCTCTTCTGATGC GACCAAGTTTTCATCTACCTCTGCTGATAAGACTGCCAAGATCTGGAGTTTTGACCTCCTTTCCCCTCTT CATGAGCTGAAGGGCCATAATGGCTGTGTCCGCTGCTCTGCCTTCTCGCTGGATGGCATCCTGTTGGCAA CTGGAGATGACAATGGAGAAATCCGGATATGGAATGTCTCAGATGGCCAGCTTCTTCATTCGTGTGCTCC GATCTCGGTAGAGGAAGGAACTGCTACCCACGGCGGCTGGGTAACTGATGTGTGCTTCTCTCCCGACAGT AAAACGCTTGTCTCTGCTGGAGGATATCTCAAGTGGTGGAATGTTGCCACTGGGGACTCCTCACAGACCT TCTACACAAATGGAACAAACCTCAAGAAAATCCACGTGTCCCCTGACTTCAGAACCTATGTGACTGTCGA TAATCTCGGTATTTTATATATTTTACAGGTTTTAGAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_009684
Insert Size	3750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009684.2, NP_033814.2
RefSeq Size	6514 bp
RefSeq ORF	3750 bp
Locus ID	11783
UniProt ID	O88879
Gene Summary	Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) encodes the longer isoform (1). Variants 1 and 2 encode the same isoform 1.

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